Nitric Oxide Donor NOC-18-Induced Changes of Mitochondrial Phosphoproteome in Rat Cardiac Ischemia Model
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Experimental Animals and Induction of Heart Ischemia
2.3. Mitochondrial Preparation
2.4. Mitochondrial Incubation with PKG
2.5. Phosphoprotein Enrichment
2.6. In-Gel Protein Digestion for Mass Spectrometry Analysis
2.7. Protein Digestion and Sample Preparation for LC–MS/MS Analysis
2.8. Liquid Chromatography and Mass Spectrometry
2.9. Data Processing, Searching, and Analysis
2.10. Statistical Data Analysis
3. Results
3.1. Mitochondrial Phosphoproteins in Response to NOC-18 versus Ischemia
3.2. Mitochondrial Proteins after Incubation with PKG
4. Discussion
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Mitochondrial Membrane-Bound Proteins | UniProt/SwissProt Number | Fold Change NOC-18/Ischemia | P-Value |
---|---|---|---|
Mitochondrial pyruvate carrier 1 (MPC1) | P63031 | 1.64 | 0.003 |
NADH dehydrogenase ubiquinone flavoprotein 2 (NDUFV2) | P19234 | −1.53 | 0.009 |
Very long chain specific acyl CoA dehydrogenase mitochondrial (ACADVL) | P45953 | 1.58 | 0.013 |
Mitochondrial 2 oxoglutarate malatecarrier protein (SLC25A11) | P97700 | 1.66 | 0.013 |
ADP/ATP translocase 1 (SLC25A4) | Q05962 | 1.57 | 0.022 |
ATP synthase subunit alpha mitochondrial (ATP5A1) | P15999 | 1.55 | 0.028 |
Trifunctional enzyme subunit beta mitochondrial (HADHB) | Q60587 | 1.61 | 0.032 |
Electron transfer flavoprotein ubiquinone oxidoreductase mitochondrial (ETFD) | Q6UPE1 | 1.63 | 0.041 |
Cytochrome c oxidase subunit 4isoform 1 mitochondrial (COX4I1) | P10888 | 1.54 | 0.042 |
Cytochrome b c1 complex subunit Rieske (UQCRFS1) | P20788 | 1.54 | 0.043 |
Mitochondrial Matrix Proteins | |||
Pyruvate dehydrogenase E1 component subunit alpha somatic form mitochondrial (PDHA1) | P26284 | 1.55 | 0.024 |
Short chain specific acyl CoAdehydrogenase mitochondrial (ACADS) | P15651 | 1.58 | 0.027 |
Long chain specific acyl CoAdehydrogenase mitochondrial (ACADVL) | P15650 | 1.59 | 0.027 |
Isovaleryl CoA dehydrogenase mitochondrial (IVD) | P12007 | 1.59 | 0.030 |
Succinyl CoA ligase ADP GDP forming subunit alpha mitochondrial (SUCLG1) | P13086 | 1.62 | 0.030 |
Lon protease homolog mitochondrial (LONP1) | Q924S5 | 1.75 | 0.032 |
Hexaprenyldihydroxybenzoatemethyltransferase mitochondrial (COQ3) | Q63159 | 1.67 | 0.032 |
Citrate synthase mitochondrial (CS) | Q8VHF5 | 1.57 | 0.034 |
3-ketoacyl CoA thiolase mitochondrial (ACAA2) | P13437 | 1.57 | 0.044 |
Malate dehydrogenase mitochondrial (MDH2) | P04636 | 1.54 | 0.049 |
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Umbrasas, D.; Jokubka, R.; Kaupinis, A.; Valius, M.; Arandarčikaitė, O.; Borutaitė, V. Nitric Oxide Donor NOC-18-Induced Changes of Mitochondrial Phosphoproteome in Rat Cardiac Ischemia Model. Medicina 2019, 55, 631. https://doi.org/10.3390/medicina55100631
Umbrasas D, Jokubka R, Kaupinis A, Valius M, Arandarčikaitė O, Borutaitė V. Nitric Oxide Donor NOC-18-Induced Changes of Mitochondrial Phosphoproteome in Rat Cardiac Ischemia Model. Medicina. 2019; 55(10):631. https://doi.org/10.3390/medicina55100631
Chicago/Turabian StyleUmbrasas, Danielius, Ramūnas Jokubka, Algirdas Kaupinis, Mindaugas Valius, Odeta Arandarčikaitė, and Vilmantė Borutaitė. 2019. "Nitric Oxide Donor NOC-18-Induced Changes of Mitochondrial Phosphoproteome in Rat Cardiac Ischemia Model" Medicina 55, no. 10: 631. https://doi.org/10.3390/medicina55100631