Controlled Release of Metformin Hydrochloride from Core-Shell Nanofibers with Fish Sarcoplasmic Protein
Abstract
:1. Introduction
2. Materials and Methods
2.1. Sarcoplasmic Protein (SP) Preparation
2.2. Preparation of Spinning Solutions
2.3. Coaxial Electrospinning
2.4. Protein Composition of FSP
2.5. Scanning Electron Microscopy (SEM)
2.6. Fourier-Transform Infrared Spectroscopy
2.7. MTT Assay
2.8. In Vitro Release Studies
2.9. In Vitro Release Kinetics
2.10. Thermal Analysis
3. Results
3.1. SDS-PAGE Assay
3.2. Morphological Characterization of Nanofibers
3.3. Fourier-Transform Infrared Spectroscopy (FTIR)
3.4. MTT Assay
3.5. Drug Release Studies and Kinetic Investigation
3.6. Thermal Behaviour
4. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Samples | Core | Shell |
---|---|---|
MH | 5% PVA + MH | 8% PLA |
MHFSP1 | 5% PVA + MH + 1% FSP | 8% PLA |
MHFSP3 | 5% PVA + MH + 3% FSP | 8% PLA |
MHFSP5 | 5% PVA + MH + 5% FSP | 8% PLA |
Sample | Zero Order | First Order | Higuchi | Korsmeyer-Peppas | ||||
---|---|---|---|---|---|---|---|---|
R2 | Ko | R2 | K1 | R2 | Kh | R2 | n | |
MH | 0.6243 | 0.001 | 0.9162 | −0.0053 | 0.79 | 2.9301 | 0.7929 | 0.3327 |
MHFSP1 | 0.6535 | 0.133 | 0.9542 | −0.0025 | 0.8407 | 3.4182 | 0.7968 | 0.4764 |
MHFSP3 | 0,7339 | 0.149 | 0.9679 | −0.0025 | 0.8944 | 3.6765 | 0.8809 | 0.3911 |
MHFSP5 | 0.7775 | 0.128 | 0.9557 | −0.0016 | 0.9191 | 3.1012 | 0.9314 | 0.2751 |
Samples | Tm (°C) | ΔHm (j/g) |
---|---|---|
Neat PLA/PVA | 165.5 | 23.48 |
MH | 165.1 | 22.96 |
MHFSP1 | 150.2 | 16.3 |
MHFSP3 | 150.5 | 11.1 |
MHFSP5 | 150.8 | 10.8 |
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Sena, S.; Sumeyra, K.N.; Ulkugul, G.; Sema, A.; Betul, K.; Muge, S.B.; Sayip, E.M.; Muhammet, U.; Cevriye, K.; Mahir, M.; et al. Controlled Release of Metformin Hydrochloride from Core-Shell Nanofibers with Fish Sarcoplasmic Protein. Medicina 2019, 55, 682. https://doi.org/10.3390/medicina55100682
Sena S, Sumeyra KN, Ulkugul G, Sema A, Betul K, Muge SB, Sayip EM, Muhammet U, Cevriye K, Mahir M, et al. Controlled Release of Metformin Hydrochloride from Core-Shell Nanofibers with Fish Sarcoplasmic Protein. Medicina. 2019; 55(10):682. https://doi.org/10.3390/medicina55100682
Chicago/Turabian StyleSena, Su, Korkmaz Nalan Sumeyra, Guven Ulkugul, Arslan Sema, Karademir Betul, Sennaroglu Bostan Muge, Eroglu Mehmet Sayip, Uzun Muhammet, Kalkandelen Cevriye, Mahirogullari Mahir, and et al. 2019. "Controlled Release of Metformin Hydrochloride from Core-Shell Nanofibers with Fish Sarcoplasmic Protein" Medicina 55, no. 10: 682. https://doi.org/10.3390/medicina55100682