Oxidant/Antioxidant Status of Breast Cancer Patients in Pre- and Post-Operative Periods
Abstract
:1. Introduction
2. Materials and Methods
2.1. Analysis of MDA, Total Glutathione (GSH + GSSG) and GST
2.2. Reagents
2.3. Statistical Analysis
3. Results
4. Discussion
5. Conclusions
Author Contributions
Conflicts of Interest
References
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Parameters | Number of Cases (n) |
---|---|
Age | |
<50 | 34 |
>50 | 18 |
Histologic subtype | |
Ductal carcinoma | 46 |
Invasive lobular carcinoma | 3 |
Other | 3 |
TNM stages | |
0 | 3 |
I | 19 |
II | 22 |
III | 8 |
Lymph node metastasis | |
Yes | 18 |
No | 34 |
Tumor grade | |
Gx | 6 |
G1 | 8 |
G2 | 23 |
G3 | 15 |
Type of surgery | |
Breast conserving surgery | 52 |
Body mass index under 50 years of age | |
<18.5 18.5–24.9 25–29.9 30–34.9 35–40 Body mass index over 50 years of age | – 15 12 5 2 |
<18.5 18.5–24.9 25–29.9 30–34.9 35–40 | – 4 9 4 1 |
Parameters | Before Surgery | Two Hours after Surgery | Five Days after Surgery | Control |
---|---|---|---|---|
MDA, nmol/mL | 11.29 ± 7.69 * | 13.93 ± 8.35 * | 9.83 ± 3.15 ● | 6.73 ± 2.66 ● |
GST, µmol/mL/min | 0.36 ± 0.16 | 0.37 ± 0.16 | 0.37 ± 0.16 ● | 0.48 ± 0.13 ● |
GSH + GSSG, µmol/mL | 0.47 ± 0.19 | 0.46 ± 0.3 | 0.48 ± 0.21 ● | 0.79 ± 0.28 ● |
Under 50 Years of Age | ||||||
Parameters | n | Before Surgery | Two Hours after Surgery | Five Days after Surgery | n | Control |
MDA, nmol/mL | 34 | 10.04 ± 5.2 | 11.68 ± 8.39 * | 8.94 ± 4.24 * | 20 | 6.06 ± 1.72 |
GST, µmol/mL/min | 34 | 0.33 ± 0.16 | 0.34 ± 0.17 | 0.33 ± 0.16 | 20 | 0.41 ± 0.19 |
GSH + GSSG, µmol/mL | 34 | 0.51 ± 0.21 | 0.46 ± 0.14 | 0.49 ± 0.24 ● | 20 | 0.98 ± 0.13 ● |
Over 50 Years of Age | ||||||
Parameters | n | Before Surgery | Two Hours after Surgery | Five Days after Surgery | n | Control |
MDA, nmol/mL | 18 | 13.67 ± 6.76 * | 18.18 ± 10.76 * | 11.46 ± 6.30 ● | 22 | 7.40 ± 1.83 ● |
GST, µmol/mL/min | 18 | 0.43 ± 0.13 | 0.44 ± 0.14 | 0.39 ± 0.17 ● | 22 | 0.55 ± 0.16 ● |
GSH + GSSG, µmol/mL | 18 | 0.41 ± 0.13 | 0.41 ± 0.11 | 0.47 ± 0.15 ● | 22 | 0.60 ± 0.17 ● |
Stage I | |||||
Parameters | n | Before Surgery | Two Hours after Surgery | Five Days after Surgery | Control |
MDA, nmol/mL | 19 | 11.99 ± 9.08 * | 16.04 ± 10.22 * | 10.52 ± 6.11 ● | 6.73 ± 2.66 ● |
GST, µmol/mL/min | 19 | 0.38 ± 0.15 | 0.39 ± 0.14 | 0.39 ± 0.13 | 0.48 ± 0.23 |
GSH + GSSG, µmol/mL | 19 | 0.48 ± 0.20 | 0.48 ± 0.09 | 0.51 ± 0.28 ● | 0.79 ± 0.28 ● |
Stage II | |||||
MDA, nmol/mL | 22 | 8.91 ± 3.47 | 10.30 ± 4.41 | 8.23 ± 3.27 | 6.73 ± 2.66 |
GST, µmol/mL/min | 22 | 0.36 ± 0.15 | 0.37 ± 0.15 | 0.37 ± 0.14 | 0.48 ± 0.23 |
GSH + GSSG, µmol/mL | 22 | 0.48 ± 0.21 | 0.43 ± 0.17 | 0.45 ± 0.17 ● | 0.79 ± 0.28 ● |
Stage III | |||||
MDA, nmol/mL | 8 | 17.11 ± 11.04 | 21.66 ± 13.82 | 14.33 ± 5.38 ● | 6.73 ± 2.66 ● |
GST, µmol/mL/min | 8 | 0.41 ± 0.16 | 0.43 ± 0.17 | 0.43 ± 0.19 | 0.48 ± 0.23 |
GSH + GSSG, µmol/mL | 8 | 0.48 ± 0.16 | 0.52 ± 0.17 | 0.56 ± 0.10 ● | 0.79 ± 0.28 ● |
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Didžiapetrienė, J.; Kazbarienė, B.; Tikuišis, R.; Dulskas, A.; Dabkevičienė, D.; Lukosevičienė, V.; Kontrimavičiūtė, E.; Sužiedėlis, K.; Ostapenko, V. Oxidant/Antioxidant Status of Breast Cancer Patients in Pre- and Post-Operative Periods. Medicina 2020, 56, 70. https://doi.org/10.3390/medicina56020070
Didžiapetrienė J, Kazbarienė B, Tikuišis R, Dulskas A, Dabkevičienė D, Lukosevičienė V, Kontrimavičiūtė E, Sužiedėlis K, Ostapenko V. Oxidant/Antioxidant Status of Breast Cancer Patients in Pre- and Post-Operative Periods. Medicina. 2020; 56(2):70. https://doi.org/10.3390/medicina56020070
Chicago/Turabian StyleDidžiapetrienė, Janina, Birutė Kazbarienė, Renatas Tikuišis, Audrius Dulskas, Daiva Dabkevičienė, Vaida Lukosevičienė, Eglė Kontrimavičiūtė, Kęstutis Sužiedėlis, and Valerijus Ostapenko. 2020. "Oxidant/Antioxidant Status of Breast Cancer Patients in Pre- and Post-Operative Periods" Medicina 56, no. 2: 70. https://doi.org/10.3390/medicina56020070