Dual Role of SIRT1 in Autophagy and Lipid Metabolism Regulation in Osteoarthritic Chondrocytes
Abstract
:1. Introduction
2. Materials and Methods
2.1. Patients and Tissue Samples
2.2. Primary Cultures of Normal and OA Human Articular Chondrocytes
2.3. Treatment of Normal Chondrocytes with siRNA against SIRT1 under Serum Starvation Conditions
2.4. Treatment of OA Chondrocytes with Resveratrol, Hydroxychloroquine, 3-Methyladenine and siRNA against SIRT1
2.5. Western Blot Analysis
2.6. Immunofluorescence
2.7. Immunoprecipitation
2.8. Statistical Analysis
3. Results
3.1. SIRT1 and LOX-1 Protein Expression Are Inversely Correlated in OA Chondrocytes
3.2. SIRT1 Regulates LOX-1 Expression via NF-κB Pathway in OA Chondrocytes
3.3. Autophagy Induction through SIRT1 in Resveratrol-Treated OA Chondrocytes
3.4. SIRT1-Mediated Regulation of LOX-1 Expression Is Autophagy-Dependent in OA Chondrocytes
3.5. SIRT1 Is Necessary for Autophagy Induction in Articular Chondrocytes
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Conflicts of Interest
References
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Papageorgiou, A.-A.; Goutas, A.; Trachana, V.; Tsezou, A. Dual Role of SIRT1 in Autophagy and Lipid Metabolism Regulation in Osteoarthritic Chondrocytes. Medicina 2021, 57, 1203. https://doi.org/10.3390/medicina57111203
Papageorgiou A-A, Goutas A, Trachana V, Tsezou A. Dual Role of SIRT1 in Autophagy and Lipid Metabolism Regulation in Osteoarthritic Chondrocytes. Medicina. 2021; 57(11):1203. https://doi.org/10.3390/medicina57111203
Chicago/Turabian StylePapageorgiou, Aliki-Alexandra, Andreas Goutas, Varvara Trachana, and Aspasia Tsezou. 2021. "Dual Role of SIRT1 in Autophagy and Lipid Metabolism Regulation in Osteoarthritic Chondrocytes" Medicina 57, no. 11: 1203. https://doi.org/10.3390/medicina57111203