New CagL Amino Acid Polymorphism Patterns of Helicobacter pylori in Peptic Ulcer and Non-Ulcer Dyspepsia
Abstract
:1. Introduction
2. Materials and Methods
2.1. Study Design
2.2. DNA Extraction
2.3. H. pylori Detection
2.4. H. pylori cagL Sequencing
2.5. Statistical Analysis
3. Results
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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CagL Amino Acid Polymorphisms | PUD † (n = 23) | NUD ‡ (n = 19) | p Value |
---|---|---|---|
22D/F | 23/0 | 19/0 | >0.05 |
32S/N | 6/17 | 4/15 | >0.05 |
35Q/K | 9/14 | 9/10 | >0.05 |
41V/A/T | 18/2/3 | 17/1/1 | >0.05 |
56A/T | 1/22 | 1/18 | >0.05 |
58D/N | 21/2 | 16/3 | >0.05 |
59K/E | 13/10 | 12/7 | >0.05 |
60M/I | 23/0 | 17/2 | >0.05 |
62E/Q | 23/0 | 18/1 | >0.05 |
84A/T | 1/22 | 0/19 | >0.05 |
114I/M | 23/0 | 19/0 | >0.05 |
122K/N | 19/4 | 15/4 | >0.05 |
134I/V | 22/1 | 15/4 | >0.05 |
154E/Q/K | 22/0/1 | 18/1/0 | >0.05 |
171A/T/V | 20/2/1 | 18/1/0 | >0.05 |
172S/P | 1/22 | 0/19 | >0.05 |
174I/V | 1/22 | 0/19 | >0.05 |
175T/I | 1/22 | 1/18 | >0.05 |
194R/K | 2/21 | 2/17 | >0.05 |
200Q/H | 22/1 | 18/1 | >0.05 |
203V/I | 0/23 | 1/18 | >0.05 |
206N/S | 0/23 | 1/18 | >0.05 |
210E/K | 1/22 | 2/17 | >0.05 |
223R/Q | 0/23 | 1/18 | >0.05 |
CagLHM Amino Acid Sequences | Total n = 42 (%) | PUD † n = 23 (%) | NUD ‡ n = 19 (%) | p Value |
---|---|---|---|---|
NEIGQ | 25 (59.52) | 13 (56.5) | 12 (63.1) | >0.05 |
NKIGQ | 11 (26.19) | 8 (34.8) | 3 (15.8) | >0.05 |
DKIGQ | 4 (9.52) | 2 (8.7) | 2 (10.5) | >0.05 |
NKMGQ | 1 (2.38) | 0 (0) | 1 (5.3) | >0.05 |
DKMGE | 1 (2.38) | 0 (0) | 1 (5.3) | >0.05 |
Patterns | CagL Amino Acid Polymorphism Combinations |
---|---|
Pattern 1 | K35/N122/V134/T175/R194/E210 |
Pattern 2 | V41/I134 |
Pattern 3 | V41/K122/A171/I174 |
Patterns | PUD † n = 23 (%) | NUD ‡ n = 19 (%) | p Value | Odds Ratio | 95% CI ‡‡ |
---|---|---|---|---|---|
1 | 6 (26.1) | 0 (0) | 0.02 | 1.353 | 1.061–1.725 |
2 | 0 (0) | 4 (17.4) | 0.03 | 1.26 | 1.004–1.597 |
3 | 0 (0) | 4 (17.4) | 0.03 | 1.26 | 1.004–1.597 |
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Caliskan, R.; Polat Sari, S.; Ercan, B.; Peker, K.D.; Omac Sonmez, M.; Akgul, O.; Sapmaz, B.; Soylu, A.; Adas, G.T.; Oner, Y.A.; et al. New CagL Amino Acid Polymorphism Patterns of Helicobacter pylori in Peptic Ulcer and Non-Ulcer Dyspepsia. Medicina 2022, 58, 1738. https://doi.org/10.3390/medicina58121738
Caliskan R, Polat Sari S, Ercan B, Peker KD, Omac Sonmez M, Akgul O, Sapmaz B, Soylu A, Adas GT, Oner YA, et al. New CagL Amino Acid Polymorphism Patterns of Helicobacter pylori in Peptic Ulcer and Non-Ulcer Dyspepsia. Medicina. 2022; 58(12):1738. https://doi.org/10.3390/medicina58121738
Chicago/Turabian StyleCaliskan, Reyhan, Silva Polat Sari, Bahadir Ercan, Kivanc Derya Peker, Mehtap Omac Sonmez, Ozer Akgul, Burcu Sapmaz, Aliye Soylu, Gokhan Tolga Adas, Yasar Ali Oner, and et al. 2022. "New CagL Amino Acid Polymorphism Patterns of Helicobacter pylori in Peptic Ulcer and Non-Ulcer Dyspepsia" Medicina 58, no. 12: 1738. https://doi.org/10.3390/medicina58121738