Deuterium-Reinforced Polyunsaturated Fatty Acids Prevent Diet-Induced Nonalcoholic Steatohepatitis by Reducing Oxidative Stress
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animal Model and Treatments
2.2. Blood Parameters
2.3. Liver Lipid Isolation and Determination
2.4. Histopathological Analysis
2.5. Cell Culture and Treatments
2.6. Cell Viability, Apoptosis, and ROS Assays
2.7. MitoSOX Assay
2.8. Quantitative Real-Time PCR (RT-qPCR) Analyses
2.9. Oil Red O Staining
2.10. Statistical Analyses
3. Results
3.1. D-PUFA Attenuates Oxidative Stress and Apoptosis Induced by TBHP in Hepatocytes
3.2. D-PUFAs Reduce the Oxidative Stress and Inflammation in Macrophages
3.3. D-PUFAs Prevent MCD-Induced Steatosis in Mice
3.4. D-PUFAs Attenuate Oxidative Stress and Inflammation Induced by MCD Diet in Mice
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Li, H.; Zhang, O.; Hui, C.; Huang, Y.; Shao, H.; Song, M.; Gao, L.; Jin, S.; Ding, C.; Xu, L. Deuterium-Reinforced Polyunsaturated Fatty Acids Prevent Diet-Induced Nonalcoholic Steatohepatitis by Reducing Oxidative Stress. Medicina 2022, 58, 790. https://doi.org/10.3390/medicina58060790
Li H, Zhang O, Hui C, Huang Y, Shao H, Song M, Gao L, Jin S, Ding C, Xu L. Deuterium-Reinforced Polyunsaturated Fatty Acids Prevent Diet-Induced Nonalcoholic Steatohepatitis by Reducing Oxidative Stress. Medicina. 2022; 58(6):790. https://doi.org/10.3390/medicina58060790
Chicago/Turabian StyleLi, Haoran, Ouyang Zhang, Chenmin Hui, Yaxin Huang, Hengrong Shao, Menghui Song, Lingjia Gao, Shengnan Jin, Chunming Ding, and Liang Xu. 2022. "Deuterium-Reinforced Polyunsaturated Fatty Acids Prevent Diet-Induced Nonalcoholic Steatohepatitis by Reducing Oxidative Stress" Medicina 58, no. 6: 790. https://doi.org/10.3390/medicina58060790