Antimicrobial Activity of Zinc against Periodontal Pathogens: A Systematic Review of In Vitro Studies
Abstract
:1. Introduction
2. Material and Methods
2.1. Protocol and Registration
2.2. Search Strategy and Data Source
2.3. Eligibility Criteria
2.4. Data Collection
2.5. Risk of Bias Assessment
3. Results
3.1. General Characteristic
3.2. Characterization of Scientific Studies
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Data Base | Keyword Formulation | Number of Publications (with Filters Applied/Number of Selected Publications) |
---|---|---|
Web of Science | Zinc AND Porphyromonas gingivalis | 69/25 |
Zinc AND Tannerella forsythia | 7/3 | |
Zinc AND Treponema denticola | 7/2 | |
Zinc AND Prevotella intermedia | 10/5 | |
Zinc AND Aggregatibacter actinomycetemcomitans | 12/4 | |
PubMed | Zinc AND Porphyromonas gingivalis | 52/16 |
Zinc AND Tannerella forsythia | 6/2 | |
Zinc AND Treponema denticola | 6/2 | |
Zinc AND Prevotella intermedia | 10/5 | |
Zinc AND Aggregatibacter actinomycetemcomitans | 15/3 | |
ScienceDirect | Zinc AND Porphyromonas gingivalis | 205/7 |
Zinc AND Tannerella forsythia | 23/4 | |
Zinc AND Treponema denticola | 31/4 | |
Zinc AND Prevotella intermedia | 50/4 | |
Zinc AND Aggregatibacter actinomycetemcomitans | 55/4 |
No. | Author, Year and Reference No. | Bacteria Examined (Amount) | Test Substance | Incubation Conditions and Duration | Results |
---|---|---|---|---|---|
1 | Dias et al., 2018 [4] | P. gingivalis, P. intermedia, and A. actinomycete mcomitans (100 µL) | ZnO | Anaerobic environment (90% nitrogen + 10% CO2); 37 °C, 24 h | Weak antibacterial activity, inhibition zone of 6.5 mm. |
ZnO + PCL | No antibacterial activity, no inhibition zone formed. | ||||
2 | Wang et al., 2019 [8] | P. gingivalis (100 µL) | ZnO nanoparticles | Anaerobic environment 37 °C, 48 h | Antibacterial activity distinguished at 40 µg/mL concentration of test substance, inhibition zone of 18.09 mm. |
3 | Mou et al., 2019 [12] | P. gingivalis (100 µL) | ZnO + albumin nanoparticles | Anaerobic environment 37 °C, 24 h | Antibacterial activity distinguished, inhibition zone of 13.36 mm. |
P. intermedia (100 µL) | Antibacterial activity distinguished, inhibition zone of 14.01 mm. | ||||
4 | Fröber et al., 2019 [13] | P. gingivalis (-) | ZnO + glucose-1- phosphate | Anaerobic environment (7.5–8% CO2) 37 °C, 24–48 h | Antibacterial activity distinguished at 100 µg/mL concentration of test substance. |
P. intermedia (-) | Antibacterial activity distinguished at 25 µg/mL concentration of test substance. | ||||
A. actinomycete mcomitans (-) | Antibacterial activity distinguished at 50 µg/mL concentration of test substance. | ||||
5 | Bergs et al., 2017 [14] | P. gingivalis (-) | ZnO + glucose-1- phosphate | Anaerobic environment (5–10% CO2) 37 °C, 24–48 h | Antibacterial activity distinguished at 100 µg/mL concentration of test substance. |
P. intermedia (-) | Antibacterial activity distinguished at 20 µg/mL concentration of test substance. | ||||
A. actinomycete mcomitans (-) | Antibacterial activity distinguished at 20 µg/mL concentration of test substance. | ||||
6 | Vargas-Reus et al., 2012 [16] | P. gingivalis (100 µL) | ZnO | Anaerobic environment, 48 h | Antibacterial activity distinguished at 250 µg/mL concentration of test substance. |
P. intermedia (100 µL) | Antibacterial activity distinguished at 100 µg/mL concentration of test substance. | ||||
A. actinomycete mcomitans (100 µL) | Antibacterial activity distinguished at 250 µg/mL concentration of test substance. | ||||
7 | Vergara-Llanos et al., 2020 [10] | P. gingivalis (-) | ZnO nanoparticles | Anaerobic environment, 37 °C, 24 h | Antibacterial activity distinguished at 78.3 µg/mL concentration of test substance. |
8 | Pizzey et al., 2011 [9] | P. gingivalis (-) | Zn gluconate | Anaerobic environment, 37 °C, 24–48 val. | Antibacterial activity distinguished at 2.76 mM concentration of test substance |
9 | Kang et al., 2017 [15] | P. gingivalis (150 µL) | ZnCl2 | Anaerobic environment 37 °C, 4–30 h | Antibacterial activity distinguished at 0.0625% concentration of test substance. |
P. intermedia (150 µL) | Antibacterial activity distinguished at 0.0625% concentration of test substance. | ||||
T.forsythia (150 µL) | Antibacterial activity distinguished at 0.125% concentration of test substance. | ||||
T.denticola (150 µL) | Antibacterial activity distinguished at 0.0625% concentration of test substance. | ||||
A. actinomycete mcomitans (150 µL) | Antibacterial activity distinguished at 0.25% concentration of test substance. | ||||
10 | Zhao et al., 2020 [17] | P. gingivalis (-) | [Zn(TBTA) (L)1.5]n | Anaerobic environment (10% CO2); 37 °C, 24 h | Antibacterial activity distinguished according to the decline of the growth curve. |
11 | Wang et al., 2019 [21] | P. gingivalis (100 µL) | Zn(NO3)2·6H2O | Anaerobic environment (80% N2, 10% H2, and 10% CO2), 24 h | Antibacterial activity distinguished according to the decline of the growth curve. |
12 | Niu et al., 2020 [11] | P. gingivalis (-) | Zn(NO3)2·6H2O | Anaerobic environment (80% N2, 10% H2, and 10% CO2), 24 h | Antibacterial activity distinguished by decrease in optical density from 0.82 up to 0.43 units of measurement. |
13 | Predoi et al., 2019 [19] | P. gingivalis (-) | ZnHApD | Anaerobic environment, 37 °C, 24–48 h | Antibacterial activity distinguished by decrease in optical density. |
14 | Shao et al., 2019 [20] | P. gingivalis and A. actinomycete mcomitans (-) | Zinc-coated titanium | 37 °C, 2 days | Antibacterial activity distinguished as no viable bacteria were detected on the surface. |
15 | Toledano-Osorio et al., 2018 [18] | P. gingivalis (-) | Zn nanoparticles | Anaerobic environment, 37 °C, 3–24 h | Antibacterial activity distinguished by 93% decrease in bacteria count. |
No. | Author, Year, and Reference No. | Test Period (h) | Inhibition Zone (mm) | Results | |||||
---|---|---|---|---|---|---|---|---|---|
P.g | P.i | T.f | T.d | A.a | For the Bacteria Studied Together | ||||
1 | Dias et al., 2018 [4] | 24 | - | - | - | - | - | 6.5 (SD 0.5) | Antibacterial activity distinguished |
2 | Wang et al., 2019 [8] | 24 | 18.09 | - | - | - | - | - | Antibacterial activity distinguished, p < 0.001 |
3 | Mou et al., 2019 [12] | 24 | 13.36 | 14.01 | - | - | - | - | Antibacterial activity distinguished, p < 0.01 |
No. | Author, Year and Reference No. | Test Period (h) | MIC/MBC (µg/mL) | Results | |||||
---|---|---|---|---|---|---|---|---|---|
P.g | P.i | T.f | T.d | A.a | For the Bacteria Studied Together | ||||
1 | Wang et al., 2019 [8] | 24 | 40/40 | - | - | - | - | - | |
2 | Vergara-Llanos et al., 2020 [10] | 24 | - | - | - | - | - | 78.3–3906 | |
3 | Frober et al., 2019 [13] | 24 | 100/100 | 25/25 | - | - | 50/50 | - | |
4 | Bergs et al., 2017 [14] | 24 | 100/100 | 20/100 | - | - | 20/100 | - | |
5 | Vargas-Reus et al., 2012 [16] | 48 | 250/250 | 100/100 | - | - | 250/250 | - | p < 0.05 |
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Griauzdyte, V.; Jagelaviciene, E. Antimicrobial Activity of Zinc against Periodontal Pathogens: A Systematic Review of In Vitro Studies. Medicina 2023, 59, 2088. https://doi.org/10.3390/medicina59122088
Griauzdyte V, Jagelaviciene E. Antimicrobial Activity of Zinc against Periodontal Pathogens: A Systematic Review of In Vitro Studies. Medicina. 2023; 59(12):2088. https://doi.org/10.3390/medicina59122088
Chicago/Turabian StyleGriauzdyte, Viktorija, and Egle Jagelaviciene. 2023. "Antimicrobial Activity of Zinc against Periodontal Pathogens: A Systematic Review of In Vitro Studies" Medicina 59, no. 12: 2088. https://doi.org/10.3390/medicina59122088