The Synergistic Effect of Zuogui Pill and Eldecalcitol on Improving Bone Mass and Osteogenesis in Type 2 Diabetic Osteoporosis
Abstract
:1. Introduction
2. Materials and Methods
2.1. Reagents
2.2. Animals and Drug Administration
2.3. Blood Glucose Assay and Serum Biochemical Analysis
2.4. Animal Euthanasia and Sample Preparation
2.5. Micro-Computed Tomography (CT) Scan
2.6. Hematoxylin and Eosin (HE) Staining
2.7. Masson Staining
2.8. Immunohistochemical and Tartrate-Resistant Acid Phosphatase (TRAP) Staining
2.9. Cell Culture, Acquisition of Medicated Serum, and Osteogenic Differentiation Induction
2.10. Alkaline Phosphatase and Alizarin Red (AR) Staining
2.11. Immunofluorescence Staining
2.12. Quantitative Real-Time Polymerase Chain Reaction (RT-qPCR) Analysis
2.13. Western Blotting
2.14. Statistical Analysis
3. Results
3.1. ZGP and ED-71 Synergistically Reduced Blood Glucose Levels in Diabetic Mice
3.2. ZGP and ED-71 Synergistically Increased Bone Mass, Improved Osteoblasts, and Inhibited Osteoclasts in Diabetic Mice
3.3. The ZGP and ED-71 Combination Promoted Osteogenic Differentiation of the MC3T3-E1 Cells
3.4. ZGP Synergized with ED-71 to Promote the Osteogenic Differentiation of MC3T3-E1 Cells through the PI3K–AKT Pathway
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Gene | Forward | Reverse |
---|---|---|
OCN | CAGAACAGACAAGTCCCACACAG | TCAGCAGAGTGAGCAGAAAGAT |
RUNX2 | TACGACCATGAGATTGGCAGTGA | TATAGGATCTGGGTGCAGGCTGA |
ALP | GCGACCACTTGAGCAAACATC | CGGCTGATTGGCTTCTTCTT |
GAPDH | GCACCGTCAAGGCTGAGAAC | TGGTGAAGACGCCAGTGGA |
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Shi, T.; Liu, T.; Kou, Y.; Rong, X.; Meng, L.; Cui, Y.; Gao, R.; Hu, S.; Li, M. The Synergistic Effect of Zuogui Pill and Eldecalcitol on Improving Bone Mass and Osteogenesis in Type 2 Diabetic Osteoporosis. Medicina 2023, 59, 1414. https://doi.org/10.3390/medicina59081414
Shi T, Liu T, Kou Y, Rong X, Meng L, Cui Y, Gao R, Hu S, Li M. The Synergistic Effect of Zuogui Pill and Eldecalcitol on Improving Bone Mass and Osteogenesis in Type 2 Diabetic Osteoporosis. Medicina. 2023; 59(8):1414. https://doi.org/10.3390/medicina59081414
Chicago/Turabian StyleShi, Tuo, Ting Liu, Yuying Kou, Xing Rong, Lingxiao Meng, Yajun Cui, Ruihan Gao, Sumin Hu, and Minqi Li. 2023. "The Synergistic Effect of Zuogui Pill and Eldecalcitol on Improving Bone Mass and Osteogenesis in Type 2 Diabetic Osteoporosis" Medicina 59, no. 8: 1414. https://doi.org/10.3390/medicina59081414