Muscle-Derived Stem/Progenitor Cells Ameliorate Acute Kidney Injury in Rats through the Anti-Apoptotic Pathway and Demonstrate Comparable Effects to Bone Marrow Mesenchymal Stem Cells
Abstract
:1. Introduction
2. Materials and Methods
2.1. Ethics
2.2. Isolation and Characterization of MDSPCs and BM-MSCs
2.3. Nephrotoxicity Model
2.4. Biochemical Functional Analysis
2.5. Biochemical Injury Markers KIM-1 and NGAL Secretion Analysis
2.6. Renal Histology
2.7. Apoptosis Assay
2.8. In Vivo Tracking of MDSPCs and BM-MSCs
2.9. Statistical Analysis
3. Results
3.1. Characteristics of MDSPCs and BM-MSCs
3.2. Effects of MDSPCs and BM-MSCs on Kidney Function after AKI
3.3. Effects of MDSPCs and BM-MSCs on KIM-1 and NGAL Secretion
3.4. Effects of MDSPCs and BM-MSCs on Kidney Histology after AKI
3.5. Anti-Apoptotic Effects of MDSPCs and BM-MSCs
3.6. Tracking of MDSPCs and BM-MSCs In Vivo
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Pavyde, E.; Usas, A.; Pockevicius, A.; Maciulaitis, R. Muscle-Derived Stem/Progenitor Cells Ameliorate Acute Kidney Injury in Rats through the Anti-Apoptotic Pathway and Demonstrate Comparable Effects to Bone Marrow Mesenchymal Stem Cells. Medicina 2024, 60, 63. https://doi.org/10.3390/medicina60010063
Pavyde E, Usas A, Pockevicius A, Maciulaitis R. Muscle-Derived Stem/Progenitor Cells Ameliorate Acute Kidney Injury in Rats through the Anti-Apoptotic Pathway and Demonstrate Comparable Effects to Bone Marrow Mesenchymal Stem Cells. Medicina. 2024; 60(1):63. https://doi.org/10.3390/medicina60010063
Chicago/Turabian StylePavyde, Egle, Arvydas Usas, Alius Pockevicius, and Romaldas Maciulaitis. 2024. "Muscle-Derived Stem/Progenitor Cells Ameliorate Acute Kidney Injury in Rats through the Anti-Apoptotic Pathway and Demonstrate Comparable Effects to Bone Marrow Mesenchymal Stem Cells" Medicina 60, no. 1: 63. https://doi.org/10.3390/medicina60010063