Assessment of Hypersensitivity to House Dust Mites in Selected Skin Diseases Using the Basophil Activation Test: A Preliminary Study
Abstract
:1. Introduction
2. Material and Methods
2.1. Patients
2.2. Procedures
2.2.1. Skin Prick Tests
2.2.2. Skin Contact Tests
2.2.3. sIgE Assay
2.3. Basophil Activation Test (BAT)
2.4. Statistical Analysis
3. Results
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Variables | Atopic Dermatitis n = 52 | Hand Eczema n = 57 | Healthy Controls n = 68 | p |
---|---|---|---|---|
age: yrs ± SD | 36.1 ± 6.9 | 40.8 ± 9.1 * | 33.2 ± 10.3 | 0.04 |
women (%) | 57.4% | 60.1% | 55.1% | <0.05 |
duration of disease: yrs ± SD | 12.9 ± 10.1 | 10.1 ± 4.7 | - | <0.05 |
BMI ± SD | 23.6 ± 2.7 | 26.8 ± 5.7 * | 24.1 ± 3.2 | 0.02 |
current or former smokers (%) | 24.6% | 28.1% | 26.8% | <0.05 |
total IgE kU/L ± SD | 456.1 ± 106.3 ** | 59.1 ± 30.3 | 44.6 ± 28.1 | 0.001 |
asthma allergy (%) | 27.1% ** | 4.7% | 0 | 0.004 |
rhinitis allergy (%) | 78.1% ** | 9.2% | 0 | 0.003 |
atopy in family (%) | 23.7% ** | 8.1% | 4.5% | 0.02 |
polysentization (%) | 39.2% ** | 1.2% | 0 | 0.003 |
inhalant allergens | ||||
most frequently | ||||
confirmed: | ||||
grass pollen | 11.5% | 1.5% | 0 | |
trees pollen | 7.4% | 1.1% | 0.5% | |
cat | 2.3% | 0 | ||
food allergy | 24.7% ** | 0 | 0 | 0.003 |
Positive Results | Atopic Dermatitis n = 52 | Hand Eczema n = 57 | Healthy Controls n = 68 | p |
---|---|---|---|---|
SPT | 28 (53.8%) | 11 (19.3%) | 1 (0.01%) | <0.05 |
sIgE | 32 (61.5%) | 6 (10.5%) | 1 (0.01%) | <0.05 |
BAT | 34 (65.4%) | 14 (24.6)%) | 0 | <0.05 |
mean percentage increase of activated CD63 basophils | 74.6% | 68.1% | 2.8% | |
correlation r * | 0.72 | 0.85 | - |
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Krupka Olek, M.; Bożek, A.; Foks Ciekalska, A.; Grzanka, A.; Kawczyk-Krupka, A. Assessment of Hypersensitivity to House Dust Mites in Selected Skin Diseases Using the Basophil Activation Test: A Preliminary Study. Medicina 2024, 60, 1608. https://doi.org/10.3390/medicina60101608
Krupka Olek M, Bożek A, Foks Ciekalska A, Grzanka A, Kawczyk-Krupka A. Assessment of Hypersensitivity to House Dust Mites in Selected Skin Diseases Using the Basophil Activation Test: A Preliminary Study. Medicina. 2024; 60(10):1608. https://doi.org/10.3390/medicina60101608
Chicago/Turabian StyleKrupka Olek, Magdalena, Andrzej Bożek, Aleksandra Foks Ciekalska, Alicja Grzanka, and Aleksandra Kawczyk-Krupka. 2024. "Assessment of Hypersensitivity to House Dust Mites in Selected Skin Diseases Using the Basophil Activation Test: A Preliminary Study" Medicina 60, no. 10: 1608. https://doi.org/10.3390/medicina60101608