A Preliminary Report Regarding the Morphological Changes of Nano-Enabled Pharmaceutical Formulation on Human Lung Carcinoma Monolayer and 3D Bronchial Microtissue
Abstract
:1. Introduction
2. Materials and Methods
2.1. Preparation of SLN Test Samples
2.2. SLN Characterization
2.2.1. Determination of the Entrapment Efficiency (EE%) and Drug-Loaded Capacity (DLC%) of the OA into the SLNs Based on OA-Loaded Green IONPs
2.2.2. Fourier Transform Infrared (FTIR) Spectroscopy Investigation of SLNs
2.2.3. Electron Microscopy Investigation
2.2.4. Hydrodynamic Diameter and Zeta Potential
2.3. In Vitro Culture Conditions
2.4. Biosafety Evaluation Using EpiAirwayTM 3D In Vitro Inserts
2.5. Histopathological Assessment of 3D Respiratory Tissue Models (EpiAirwayTM)
2.6. Cell Viability Evaluation
2.7. Statistical Analysis
3. Results
3.1. Physico-Chemical Characterization of SLN
3.1.1. Entrapment Efficiency (EE%) and Drug Loading Capacity (DLC%) of the OA in the SLNs Based on OA-Loaded Green IONPs
3.1.2. FTIR Characterization of SLNs
3.1.3. Electron Microscopy Investigations
3.1.4. DLS Measurements
3.2. Biosafety Profile Using EpiAirwayTM Microtissue
3.3. Histopathological Evaluation of 3D Microtissues
3.4. Cytotoxic Potential on A549 2D Cell Culture
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Sample | Free OA [μg] | EE [%] | DLC [%] |
---|---|---|---|
SLN_CS 25@OA | 195.5 | 80.45 | 1.226 |
SLN_CS 80@OA | 167.7 | 83.23 | 1.269 |
SLN_OB 25@OA | 95.7 | 90.43 | 1.379 |
SLN_OB 80@OA | 63.8 | 93.62 | 1.427 |
Test Sample | Hd (nm) | PDI | ζ-Potential (mV) |
---|---|---|---|
SLN_CS 25@OA | 75.3 | 0.283 | −29.2 |
SLN_CS 80@OA | 78.2 | 0.366 | −38.5 |
SLN_OB 25@OA | 66.5 | 0.189 | −20.7 |
SLN_OB 80@OA | 74.8 | 0.276 | −28.4 |
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Prodan-Bărbulescu, C.; Watz, C.-G.; Moacă, E.-A.; Faur, A.-C.; Dehelean, C.-A.; Faur, F.I.; Grigoriţă, L.O.; Maghiari, A.L.; Tuţac, P.; Duţă, C.; et al. A Preliminary Report Regarding the Morphological Changes of Nano-Enabled Pharmaceutical Formulation on Human Lung Carcinoma Monolayer and 3D Bronchial Microtissue. Medicina 2024, 60, 208. https://doi.org/10.3390/medicina60020208
Prodan-Bărbulescu C, Watz C-G, Moacă E-A, Faur A-C, Dehelean C-A, Faur FI, Grigoriţă LO, Maghiari AL, Tuţac P, Duţă C, et al. A Preliminary Report Regarding the Morphological Changes of Nano-Enabled Pharmaceutical Formulation on Human Lung Carcinoma Monolayer and 3D Bronchial Microtissue. Medicina. 2024; 60(2):208. https://doi.org/10.3390/medicina60020208
Chicago/Turabian StyleProdan-Bărbulescu, Cătălin, Claudia-Geanina Watz, Elena-Alina Moacă, Alexandra-Corina Faur, Cristina-Adriana Dehelean, Flaviu Ionut Faur, Laura Octavia Grigoriţă, Anca Laura Maghiari, Paul Tuţac, Ciprian Duţă, and et al. 2024. "A Preliminary Report Regarding the Morphological Changes of Nano-Enabled Pharmaceutical Formulation on Human Lung Carcinoma Monolayer and 3D Bronchial Microtissue" Medicina 60, no. 2: 208. https://doi.org/10.3390/medicina60020208