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Article

GSTM1 and GSTP1 Polymorphisms Affect Outcome in Colorectal Adenocarcinoma

by
Milica Stojkovic Lalosevic
1,2,
Vesna Coric
2,3,
Tatjana Pekmezovic
2,4,
Tatjana Simic
2,3,
Aleksandra Pavlovic Markovic
1,2,* and
Marija Pljesa Ercegovac
2,3,*
1
Clinic of Gastroenterology and Hepatology, University Clinical Center of Serbia, 11000 Belgrade, Serbia
2
Faculty of Medicine, University of Belgrade, 11000 Belgrade, Serbia
3
Institute of Medical and Clinical Biochemistry, 11000 Belgrade, Serbia
4
Institute of Epidemiology, 11000 Belgrade, Serbia
*
Authors to whom correspondence should be addressed.
Medicina 2024, 60(4), 553; https://doi.org/10.3390/medicina60040553
Submission received: 14 February 2024 / Revised: 20 March 2024 / Accepted: 24 March 2024 / Published: 28 March 2024
Browse Figures
Versions Notes

Abstract

:
Background and Objectives: Despite improvements in screening programs, a large number of patients with colorectal cancer (CRC) are diagnosed in an advanced disease stage. Previous investigations imply that glutathione transferases (GSTs) might be associated with the development and progression of CRC. Moreover, the detoxification mechanism of oxaliplatin, which represents the first line of treatment for advanced CRC, is mediated via certain GSTs. The aim of this study was to evaluate the significance of certain GST genetic variants on CRC prognosis and the efficacy of oxaliplatin-based treatment. Materials and Methods: This prospective study included 523 patients diagnosed with CRC in the period between 2014 and 2016, at the Digestive Surgery Clinic, University Clinical Center of Serbia, Belgrade. Patients were followed for a median of 43.47 ± 17.01 months (minimum 1–63 months). Additionally, 109 patients with advanced disease, after surgical treatment, received FOLFOX6 treatment as a first-line therapy between 2014 and 2020. The Kaplan–Meier method was used to analyze cumulative survival, and the Cox proportional hazard regression model was used to study the effects of different GST genotypes on overall survival. Results: Individuals with the GSTM1-null genotype and the GSTP1 IleVal+ValVal (variant) genotype had significantly shorter survival when compared to referent genotypes (GSTM1-active and GSTP1 IleIle) (log-rank: p = 0.001). Moreover, individuals with the GSTM1-null genotype who received 5-FU-based treatment had statistically significantly shorter survival when compared to individuals with the GSTM1-active genotype (log-rank: p = 0.05). Conclusions: Both GSTM1-null and GSTP1 IleVal+ValVal (variant) genotypes are associated with significantly shorter survival in CRC patients. What is more, the GSTM1-null genotype is associated with shorter survival in patients receiving FOLOFOX6 treatment.

1. Introduction

Colorectal cancer (CRC) is the leading gastrointestinal malignancy worldwide, with an estimated 1.9 million new cases and 935,000 CRC-related deaths in 2020 [1]. The burden of CRC is on the rise globally, with Europe being one of the regions with the highest incidence and prevalence [2]. Despite improvements in screening programs, a great number of individuals are still being diagnosed in advanced disease stages [3]. The average 5-year survival rate of CRC patients remains at approximately 65%, which further emphasizes the need for establishing biomarkers of CRC progression [4].
Understanding the genetic foundation of CRC has been the focus of interest in numerous studies. The predominant type of CRC is adenocarcinoma, originating from the colon epithelium [5]. The process of carcinogenesis is stepwise and affected by multiple mutations through the previously described adenoma–carcinoma sequence [6]. Indeed, it has been suggested that CRC development is multifactorial in the majority of cases and has been attributed to a combination of sporadic mutations and environmental contributing factors [7]. Lifestyle habits, such as obesity, drinking, and smoking, are recognizable factors in CRC formation [8]. In contrast to rather well-known genetic and modifiable risk factors involved in CRC development, data regarding prognostic genetic factors are nevertheless scarce.
Earlier studies imply that glutathione transferases might be associated with both the development and the progression of CRC [9,10]. Glutathione transferases (EC 2.5.1.18), which are also known as glutathione S-transferases or GSTs, are enzymes with multiple functions and are engaged in a variety of activities, both catalytic and non-catalytic [11]. GSTs are generally considered the primary enzymes of the Phase II cellular detoxification system. To elaborate, GSTs are widely recognized for their ability to facilitate the nucleophilic addition of glutathione (GSH) to a diverse array of nonpolar compounds, whether they are of exogenous or endogenous origin [12]. These compounds contain electrophilic functional groups, which make the products more water-soluble, making it easier for Phase III enzymes of the cellular detoxification system to remove them from the cell [11]. Cytosolic GSTM1 and GSTT1 are especially significant in the biotransformation of polycyclic aromatic hydrocarbons, which can be detected in processed meat and cigarette smoke, which are well-recognized contributing factors to CRC development [13]. Aside from their function in biotransformation reactions, GSTs participate in processes of cellular survival, growth, and death. This is achieved via protein-protein interactions with particular signaling molecules. Specifically, GSTs have been demonstrated to exert a negative regulatory effect on protein kinases such as c-Jun NH2-terminal kinase (JNK1) and apoptosis signal-regulating kinase 1 (ASK1) [14]. GSTs are highly polymorphic in the general population, with polymorphisms leading to the modification of enzymatic activity [15]. Precisely, both deletional and single-nucleotide polymorphisms (SNPs) are responsible for the complete absence or alteration of enzyme activity [15]. Regarding CRC, several studies have implied that GSTs might play a role, not only in the development but also in CRC progression [16,17,18]. However, data regarding the potential role of different GST genetic variants are inconsistent and require further investigation [18].
The first-line therapy in the treatment of patients with advanced colorectal cancer includes the FOLFOX6 regimen, which represents a combination of 5-fluorouracil/leucovorin (5FU/LV) and oxaliplatin [19]. Oxaliplatin expresses its chemotherapeutic effects by generating DNA adducts of platinum derivate with the DNA base, further resulting in programmed cellular death [17]. Furthermore, oxaliplatin-based chemotherapy is shown to contribute to oxidative stress. Since the detoxification mechanism of oxaliplatin is mediated via certain GSTs, their polymorphic expression could lead to an alteration in the response to applied chemotherapeutics [20]. Bearing this in mind, it might be speculated that GST polymorphisms might serve as potential biomarkers in the prediction of oxaliplatin-based treatment efficacy [21].
Taking into consideration that GST polymorphisms might play an important role in CRC progression but could also affect the treatment outcome, the aim of this study was to evaluate the possible role of certain GST genetic variants in CRC prognosis, as well as whether it might affect the efficacy of oxaliplatin-based treatment in these patients.

2. Materials and Methods

2.1. Data Source and Patient Selection

Patients included in this study had clinically (via colonoscopy) and histopathologically verified presence of CRC. Exclusion criteria were a previous history of another malignancy, including recurrent CRC, or a desire of the patient to no longer participate in the study. The CRC histopathological confirmation was consistent with the TNM and Dukes classification [22].

2.2. Study Design

This prospective study included 523 patients diagnosed with CRC in the period between 2014 and 2016, at the Digestive Surgery Clinic, University Clinical Center of Serbia, Belgrade. Patients were followed for a median of 43.47 ± 17.01 months (minimum 1–63 months). Data regarding age, sex, body mass index, and smoking status, as well as primary localization of the carcinoma, TNM stage, differentiation, and perineural and lymphovascular invasion, were collected.
In our cohort, 109 patients with advanced disease received FOLFOX6 treatment as the first-line therapy after surgical treatment between 2014 and 2020. FOLFOX6 treatment consisted of 2 h infusion of oxaliplatin (100 mg/m2), 2 h infusion of leucovorin (400 mg/m2), and a bolus of 5 FU (400 mg/m2) followed by 5 FU (2500 mg/m2) over 46 h in a continuous infusion [23]. The patients received this treatment every two weeks unless the disease progressed.
A structured questionnaire developed at the Institute of Epidemiology, Faculty of Medicine, University in Belgrade, was used for collecting patients’ data regarding socio-demographic factors. This study was approved by the Institutional Ethical Board (approval number 56-6, University Clinical center of Serbia) and performed according to the principles of the Helsinki Declaration. Written consent was obtained prior to inclusion in the study.
Overall survival (OS) was expressed as the time from surgery until the date of the last follow-up (1 June 2020) or the time of death. Follow-up data were obtainable for 471 patients with CRC, while the contact information for 52 patients (9%) was lost. The treatment response was evaluated according to the response evaluation criteria in solid tumors (RECIST) guidelines [24]. In addition, after every three cycles of FOLFOX6 treatment, each patient underwent a diagnostic algorithm consisting of an abdominal ultrasound and a CT chest–abdomen–pelvis protocol.

2.3. Peripheral Blood Collection and Assay

The peripheral blood of patients with CRC was used for DNA isolation by QIAamp DNA Blood Mini Kit (Qiagen, #51306, Chatsworth, CA, USA) according to the protocol of the manufacturer. As described by Abdel-Rahman et al., multiplex PCR was utilized to identify homozygous deletions of GSTM1 and GSTT1 [25]. For the detection of GSTA1 C69T (rs3957357) SNP, in accordance with Ping et al., the PCR-restriction fragment length polymorphism (RFLP) approach using the Eam1104I (Thermo Fisher Scientific, Waltham, MA, USA) restriction enzyme was used [26]. The representative findings analyzed on the appropriate agarose gels are presented in Supplementary Figures S1 and S2. The Applied Biosystems TaqMan® Drug Metabolism Genotyping assay (Life Technologies, Applied Biosystems, Carlsbad, CA, USA, assay ID: C 3,237,198 20) was utilized to analyze the GSTP1 Ile105Val (rs1695) SNP according to the manufacturer’s protocols.

2.4. Statistical Analysis

Statistical analysis was performed using SPSS ver. 20.0 (IBM Corporation, Armonk, NY, USA) [27]. The genotype distribution was assessed for the presence of deviation from Hardy–Weinberg equilibrium. The cumulative survival estimation was based on the Kaplan–Meier method. The Cox proportional hazard regression model was used to study the effects of different GST genotypes on overall survival. In addition, models have been adapted by covariates. Model 1 was adjusted to sex and age, Model 2 to covariates of Model 1 as well as obesity and smoking, Model 3 to covariates of Model 2 and FOLFOX6 treatment, and Model 4 to tumor characteristics: localization and differentiation. The results were regarded as statistically significant if the p-value was ≤0.050.

3. Results

Selected patients’ characteristics are outlined in Table 1. Among 471 CRC patients with successfully acquired follow-up data, there were 131 deaths (28%) during the follow-up period. A statistically significant difference in terms of patients’ outcomes was observed regarding the TNM classification and grade of the primary tumor (p = 0.001 and p = 0.005, respectively). Namely, the frequency of the lethal outcome was higher in patients with higher-stage and -grade colorectal cancer. On the other hand, no association was observed regarding colorectal cancer side localization. Of note, the predominant localization of CRC was the rectum (55%), and, in the majority of cases, the tumor was well-differentiated (77%), while the T3 stage (49%) was the most frequent one.

3.1. GST Genotypes and Survival

Table 2 represents the possible association between different GST genotypes and outcomes in patients with colorectal carcinoma during the follow-up period.
As presented, there was a statistically significant difference in patients’ outcomes depending on the presence of different GST genetic variants (p = 0.001 and p = 0.009, respectively). Precisely, individuals with GSTM1-null and GSTP1 IleVal+ValVal (variant) genotypes had significantly higher frequencies of lethal outcomes when compared to carriers of GSTM1-active and GSTP1 IleIle (referent) genotypes. Regarding the other two investigated polymorphisms, no significant association was observed between GSTT1 and GSTA1 genetic variants and disease outcomes in CRC patients.

3.2. Effect of GST Polymorphisms on CRC Patients’ Overall Survival

The Hardy–Weinberg equilibrium (HWE) was assessed indicating a deviation only for GSTP1 rs1695 (the chi-squared value of 6.029 and the chi-squared test p-value of 0.014). Cox regression analysis confirmed the significance of both GSTM1-null and GSTP1 IleVal+ValVal (variant) genotypes as independent prognostic factors for increased overall mortality in patients with colorectal cancer. When analyzed in four different models, both GSTM1-null and GSTP1 IleVal+ValVal (variant) genotypes showed significant multivariable-adjusted HR (Table 3, Table 4, Table 5 and Table 6), while regarding other GST genotypes, the obtained results were not statistically significant (p > 0.05).
Namely, taking into consideration covariates that might contribute to CRC patients’ prognosis (age, sex, lifestyle habits, treatment, and tumor characteristic), GSTM1-null and GSTP1 IleVal+ValVal (variant) exhibited a significant prognostic effect in all assessed models, indicating a higher risk of mortality in carriers of these genotypes. Indeed, when the analysis of the effect of GST genetic variants on CRC patients’ overall survival was conducted using Model 1 (adjustment to age and sex), the obtained results clearly indicated that the risk of mortality was significantly higher (HR 1.81, 95% CI 1.22–2.68, p = 0.003) in individuals lacking the GSTM1 protein when compared to those with the intact protein presence. In this line, the risk of mortality was 1.53 times significantly higher (95% CI 0.67–2.46) in carriers of the GSTP1-variant (IleVal or ValVal) genotype in comparison to carriers of the GSTP1-referent genotype (p = 0.048). However, such an effect was not observed in the case of either GSTT1 or GSTA1 genotypes (Table 3).
The next model applied in the analysis, Model 2, apart from adjustments to age and sex, also included known CRC risk factors, such as smoking and BMI. The prognostic effect of GST genotypes recognized using Model 1 remained. Namely, the GSTM1-null genotype increased the risk of mortality by 1.79 times (p = 0.04), while the GSTP1-variant genotype was associated with a 1.57 times higher mortality risk in CRC patients (p = 0.035) in comparison to the reference GSTM1-active and GSTP1-referent IleIle genotypes, respectively (Table 4).
The statistical model presented in Table 5, Model 3, is adjusted to covariates of Model 2, but also includes information regarding the applied FOLFOX6 treatment in patients with colorectal cancer. Similarly to results obtained in the two previous models, genetic variations in GSTM1 and GSTP1 exhibited a prognostic effect in terms of mortality risk in CRC patients, while the observed effect was lacking in cases of GSTT1 and GSTA1 polymorphisms.
Last, but not least, Model 4 included adjustments to tumor characteristics (colorectal cancer localization and differentiation) in the analysis of the prognostic effect of GST genetic variations on the risk of overall mortality in CRC patients (Table 6). The obtained results resemble the results from the analysis using previous models, further highlighting the role of GSTM1 and GSTP1 polymorphisms as possible determinants of mortality risk in patients with colorectal cancer (HR 1.89, p = 0.001 and HR 1.50, p = 0.046, respectively).

3.3. The Relevance of GST Polymorphisms in the Overall Survival of CRC Patients

Kaplan–Meier analysis showed statistically significantly shorter overall survival in individuals with the GSTM1-null genotype when compared to carriers of the GSTM1-active genotype (log-rank: p = 0.001, Figure 1b). Additionally, the Kaplan–Meier analysis showed statistically significant shorter overall survival in carriers of the GSTP1 IleVal+ValVal (variant) genotype in comparison to individuals with the GSTP1 IleIle (referent) genotype (log-rank: p = 0.001, Figure 1d). However, no statistically significant effect of either GSTA1 or GSTT1 polymorphisms was observed in terms of overall survival (Figure 1a,c) in CRC patients (p > 0.05).

3.4. Effects of GST Polymorphisms on the Overall Survival of CRC Patients on 5-FU-Based Treatment

In the next step, we further analyzed the potential significance of certain GST genetic variants on the efficacy of oxaliplatin-based treatment in CRC patients since GSTs participate in the metabolism of the applied drug. Interestingly, Kaplan–Meier analysis showed statistically significant shorter overall survival in individuals with the GSTM1-null genotype who received 5-FU-based treatment when compared to individuals with the GSTM1-active genotype (log-rank: p = 0.05, Figure 2b), which was not observed for other investigated genotypes (log-rank: p > 0.05, Figure 2a,c,d). Surprisingly, in our group of patients with colorectal cancer, no association was observed in the case of GSTP1, which is the class of glutathione transferases known for its role in drug metabolism, as well as the effect on chemoresistance development, which has previously been associated with the metabolism of platinum derivatives.

4. Discussion

In this study, we have assessed the effect of four common GST polymorphisms in terms of CRC prognosis. Our results suggested that individuals with the GSTM1-null genotype, as well as individuals with the GSTP1 IleVal+ValVal (variant) genotype, have significantly shorter overall survival in comparison to individuals with the corresponding referent genotypes. In addition, when the association between different GST genetic variants and overall mortality was analyzed by applying the specified and adjusted statistical Models, GSTM1-null and GSTP1-variant genotypes were recognized as independent prognostic factors for increased overall mortality. Furthermore, carriers of the GSTM1-null genotype who underwent treatment with FOLFOX6 had significantly shorter overall survival compared to individuals with the GSTM1-active genotype.
For years, the role of GST genetic polymorphisms in patients with CRC has drawn researchers’ attention worldwide. The rationale for this is the fact that environmental factors are recognized as significant contributing factors in CRC development and progression, while GSTs are known for their role in xenobiotic detoxification and inactivation. [13,28]. However, available data regarding a possible association between GST polymorphisms and CRC development and progression are still quite controversial [16,27,29].
Glutathione transferase M1 is among the most extensively studied GST polymorphisms associated with cancer development and progression in general. Deletion polymorphism of the GSTM1 gene leads to the complete absence of protein, meaning a complete lack of enzyme activity, which could further result in increased susceptibility to carcinoma development in carriers of the GSTM1-null genotype [30]. Although several meta-analyses have investigated the risk of CRC development in carriers of GSTM1-active vs. GSTM1-null genotypes, the obtained results are still debatable [31,32]. Ethnicity, as well as geographical region, could be among the main factors influencing these conflicting data [33]. On the other hand, studies regarding the possible role of the GSTM1 genotype as a prognostic biomarker in patients with CRC are scarce. Csejtei et al. found that patients with the GSTM1-null genotype have significantly lower survival when compared to individuals with the GSTM1-active genotype, which is in accordance with our study [34]. In this line, Liu et al. concluded that the GSTM1-null genotype is associated with shorter overall survival in Caucasian patients with CRC [31]. Furthermore, Feng et al. recently suggested that GSTM1 might be associated with outcomes in CRC patients [35]. All these results justify the researchers’ focus on investigating the differential roles of GSTM1 in colorectal cancer.
Gene deletion of another glutathione transferase, GSTT1, also results in the complete lack of this protein in the gastrointestinal tract, significantly affecting and disabling the detoxification ability in individuals carrying the GSTT1-null genotype. For that reason, the GSTT1-null genotype is another of the most frequently investigated GST genotypes [23,24,25]. Although the GSTT1-null genotype has so far been associated with CRC susceptibility in Caucasians, data regarding its effect on overall survival in patients with CRC are not so abundant [36]. Indeed, Liu et al. conducted a meta-analysis that included 13 articles on the association between GSTT1 polymorphism and gastric or colorectal cancer outcomes and found a lack of data when it comes to overall survival in Caucasian patients with CRC, which is in agreement with our results [31].
An additional focus of our study was the assessment of GSTA1 polymorphism in CRC, considering that it has been studied far less than other GST genetic variants. One of the first studies investigating the association between GSTA1 polymorphism and CRC development was that of Martinez et al., which showed that GSTA1 polymorphic expression does not influence CRC susceptibility [37]. Although meta-analyses regarding GSTA1 genetic variability and susceptibility to CRC development were conducted, studies regarding the association with the outcome in CRC patients are lacking [9,10]. Our results suggest that there is no significant effect of GSTA1 rs 3,957,357 genetic variation on overall survival in patients with CRC.
Due to its role in chemoresistance, GSTP1 is by far the most extensively investigated glutathione transferase in cancers. Namely, GSTP1 is shown to be overexpressed in numerous tumors, including CRC [38], implying that it plays an important role in the complex processes of carcinogenesis [10]. However, when it comes to the results on the association between GSTP1 genotypes and the survival of CRC patients, the available data seem rather conflicting. In the recent study by Rodriguez-Fleming et al. [16], GSTP1 polymorphism was not associated with the survival of patients with CRC, which is not in accordance with our results. Indeed, we have found that the presence of different GSTP1 genetic variants affects survival in CRC patients in terms of shorter overall survival in carriers of the GSTP1 IleVal+ValVal (variant) genotype when compared to individuals with the GSTP1 IleIle (referent) genotype. The possible explanation for this discrepancy in our findings may be due to the fact, which was previously mentioned, that the distribution of different GSTP1 genotypes might vary between people of different geographical and ethnic origins. Furthermore, in the aforementioned study, the sample size might be of influence, as it consisted of around 200 individuals as opposed to our cohort, which included over 500 patients.
Since previous findings have indicated that cancer cells in general might have a higher level of GST expression, which could potentially influence the detoxification of anticancer therapy [39], this seemed significant in terms of colorectal cancer treatment. FOLFOX6 treatment represents the first-line therapy for CRC in patients with advanced disease and the response rate varies at around 40% [40]. Considering the high extent of response variability among patients, the meta-analysis by Shahnam et al. evaluated the role of different genetic polymorphisms in patients’ responses to oxaliplatin-based therapy, as well as in the survival of patients with CRC [41]. Among the 32 studies that were included in this investigation, only 3 analyzed the presence of GSTM1, either in Asian or Caucasian populations, while the data regarding the overall survival in CRC patients were actually lacking. However, the results of McLeod et al. imply that individuals with the GSTM1-null genotype had significantly higher numbers of adverse effects and lower survival rates [42]. Additionally, although statistical significance was lacking, the results of Boige et al. indicated shorter overall survival in patients with the GSTM1-null genotype [43]. The results of our study, which suggest that individuals with the GSTM1-null genotype who received 5-FU-based treatment had significantly shorter overall survival compared to individuals with the GSTM1-active genotype, are in concordance with both mentioned studies [42,43].
Another GST polymorphism that was analyzed in CRC patients receiving FOLFOX6 treatment can be seen in the study of Stoehlamacher et al., who found that the GSTP1 IleVal genotype is associated with increased survival in patients receiving this treatment [44]. This result is not in agreement with our results, possibly since, in our study, we analyzed the presence of at least one variant GSTP1 allele or, more precisely, either the IleVal or ValVal genotype. Although certain traditional anti-cancer medications like cisplatin can be affected by GST expression and deactivated through a process called conjugation with glutathione, there are also other potential mechanisms through which GSTs could contribute to the development of resistance to anti-cancer medications [45]. Indeed, a multitude of anti-cancer substances trigger the process of apoptosis by activating the kinase pathway, particularly involving JNK and p38. What is more, cisplatin is a medication that relies on JNK activity to achieve its maximum cytotoxic effect in a way that the suppression of the JNK signaling pathway results in a reduction in cisplatin-induced cell death [12]. However, the impact of polymorphic expression of GSTP1 on the apoptosis that is dependent on JNK1 has not been explained so far. There has only been one study that has demonstrated that the GSTP1 Val allele is a more effective JNK1 inhibitor and thus has a stronger antiapoptotic impact than the wild-type Ile allele [46].
Several constraints of this investigation necessitate attention. Loss to follow-up can introduce bias into the assessment of association. Furthermore, in order to gain further insight into the impact of GST polymorphisms on overall survival, it would be advantageous to explore its potential correlation with cancer-specific survival in a broader group of individuals. Additionally, conducting future research on a significantly larger study group, which would include individuals with different ethnicities and geographic origins to investigate the collective impact of GST polymorphisms on the prognosis of colorectal cancer patients, would be highly helpful.

5. Conclusions

This study supports the hypothesis that GST polymorphisms might have an effect on the overall survival of patients with colorectal cancer. The GSTM1-null genotype and the GSTP1 IleVal+ValVal (variant) genotype are found to be associated with significantly shorter survival in CRC patients. Additionally, the GSTM1-null genotype affects the survival of patients receiving FOLFOX6 treatment. Further studies are necessary to shed more light on the supposed role of GST genetic variants in the prognosis of patients with colorectal cancer.

Supplementary Materials

The following supporting information can be downloaded at: https://www.mdpi.com/article/10.3390/medicina60040553/s1, Figure S1. 2% agarose gel electrophoresis: PCR genotyping for GSTM1 and GSTT1 polymorphisms. Lane 1 comprises DNA marker (ladder). Lanes 3, 5, 7, 8, 11 comprise PCR products of patients with the GSTT1-active/GSTM1-active genotype; Lanes 2, 4, 10 comprise PCR products of patients with the GSTT1-active/GSTM1-null genotype; Lane 6 and 9 indicate patients with GSTT1-null/GSTM1-null genotype; CYP1A1 was used as housekeeping gene (present in lanes 2–11); Figure S2. 3% agarose gel electrophoresis: PCR-RFLP restriction products of the GSTA1 gene. Lanes 1, 2, 4, 8, 10, 11, 14 comprise PCR products of patients with the GSTA1*CC genotype; Lanes 5, 6, 7, 9, 13 comprise PCR-RFLP restriction products of patients with the GSTA1*CT genotype; Lanes 3 and 12 comprise RFLP-PCR restriction products of patients with GSTA1*TT genotype. Lane 15 comprises DNA marker (ladder). The Applied Biosystems TaqMan® Drug Metabolism Genotyping assay (Life Technologies, Applied Biosystems, USA, assay ID: C 323719820) was utilized to analyze the GSTP1 Ile105Val (rs1695), and was assessed using Eppendorf Mastercycler ep® realplex, according to the manufacturer protocol.

Author Contributions

Conceptualization, M.P.E., A.P.M., T.S., M.S.L. and V.C.; methodology, T.P.; software, V.C.; validation, M.P.E. and A.P.M.; formal analysis, T.S.; investigation, M.S.L.; resources, M.P.E.; data curation, M.S.L.; writing—original draft preparation, M.S.L. and V.C.; writing—review and editing, V.C., M.P.E. and A.P.M.; visualization, T.S.; supervision, A.P.M., M.P.E. and T.S.; project administration, T.P.; funding acquisition, T.S. All authors have read and agreed to the published version of the manuscript.

Funding

This work was supported by the Ministry of Education, Science and Technological Development of the Republic of Serbia (Grant No. 200110).

Institutional Review Board Statement

This study was approved by the Institutional Ethical Board (approval number 56-6, date 12 January 2016, University Clinical center of Serbia) and performed according to the principles of the Helsinki declaration.

Informed Consent Statement

Informed consent was obtained from all subjects involved in the study. Written informed consent has been obtained from the patient(s) to publish this paper.

Data Availability Statement

The data supporting the reported results can be found upon request in the form of datasets available at the Clinic of Gastroenterohepatology, University Clinical Centre of Serbia and at the Institute of Medical and clinical Biochemistry, Faculty of Medicine University of Belgrade.

Conflicts of Interest

The authors declare no conflicts of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results.

References

  1. Sung, H.; Ferlay, J.; Siegel, R.L.; Laversanne, M.; Soerjomataram, I.; Jemal, A.; Bray, F. Global Cancer Statistics 2020: GLOBOCAN Estimates of Incidence and Mortality Worldwide for 36 Cancers in 185 Countries. CA Cancer J. Clin. 2021, 71, 209–249. [Google Scholar] [CrossRef] [PubMed]
  2. Douaiher, J.; Ravipati, A.; Grams, B.; Chowdhury, S.; Alatise, O.; Are, C. Colorectal cancer-global burden, trends, and geographical variations. J. Surg. Oncol. 2017, 115, 619–630. [Google Scholar] [CrossRef] [PubMed]
  3. Tauriello, D.V.F.; Calon, A.; Lonardo, E.; Batlle, E. Determinants of metastatic competency in colorectal cancer. Mol. Oncol. 2017, 11, 97–119. [Google Scholar] [CrossRef] [PubMed]
  4. Moghimi-Dehkordi, B.; Safaee, A. An overview of colorectal cancer survival rates and prognosis in Asia. World J. Gastrointest. Oncol. 2012, 4, 71–75. [Google Scholar] [CrossRef] [PubMed]
  5. Guo, E.; Wei, H.; Liao, X.; Wu, L.; Zeng, X. Clinical significance and biological mechanisms of glutathione S-transferase mu gene family in colon adenocarcinoma. BMC Med. Genet. 2020, 21, 130. [Google Scholar] [CrossRef] [PubMed]
  6. Fearon, E.R.; Vogelstein, B. A genetic model for colorectal tumorigenesis. Cell 1990, 61, 759–767. [Google Scholar] [CrossRef] [PubMed]
  7. Giglia, M.D.; Chu, D.I. Familial Colorectal Cancer: Understanding the Alphabet Soup. Clin. Colon Rectal Surg. 2016, 29, 185–195. [Google Scholar] [CrossRef] [PubMed]
  8. Haggar, F.A.; Boushey, R.P. Colorectal cancer epidemiology: Incidence, mortality, survival, and risk factors. Clin. Colon Rectal Surg. 2009, 22, 191–197. [Google Scholar] [CrossRef] [PubMed]
  9. Economopoulos, K.P.; Sergentanis, T.N. GSTM1, GSTT1, GSTP1, GSTA1 and colorectal cancer risk: A comprehensive meta-analysis. Eur. J. Cancer 2010, 46, 1617–1631. [Google Scholar] [CrossRef] [PubMed]
  10. Hezova, R.; Bienertova-Vasku, J.; Sachlova, M.; Brezkova, V.; Vasku, A.; Svoboda, M.; Radová, L.; Kiss, I.; Vyzula, R.; Slaby, O. Common polymorphisms in GSTM1, GSTT1, GSTP1, GSTA1 and susceptibility to colorectal cancer in the Central European population. Eur. J. Med. Res. 2012, 17, 17. [Google Scholar] [CrossRef] [PubMed]
  11. Mannervik, B.; Danielson, U.H. Glutathione transferases-structure and catalytic activity. CRC Crit. Rev. Biochem. 1988, 23, 283–337. [Google Scholar] [CrossRef]
  12. Tew, K.D.; Townsend, D.M. Glutathione-s-transferases as determinants of cell survival and death. Antioxid. Redox Signal. 2012, 17, 1728–1737. [Google Scholar] [CrossRef] [PubMed]
  13. Koh, W.-P.; Nelson, H.H.; Yuan, J.-M.; Van den Berg, D.; Jin, A.; Wang, R.; Yu, M.C. Glutathione S-transferase (GST) gene polymorphisms, cigarette smoking and colorectal cancer risk among Chinese in Singapore. Carcinogenesis 2011, 32, 1507–1511. [Google Scholar] [CrossRef] [PubMed]
  14. Board, P.G.; Menon, D. Glutathione transferases, regulators of cellular metabolism and physiology. Biochim. Biophys. Acta 2013, 1830, 3267–3288. [Google Scholar] [CrossRef]
  15. Grussy, K.; Łaska, M.; Moczurad, W.; Król-Kulikowska, M.; Ściskalska, M. The importance of polymorphisms in the genes encoding glutathione S-transferase isoenzymes in development of selected cancers and cardiovascular diseases. Mol. Biol. Rep. 2023, 50, 9649–9661. [Google Scholar] [CrossRef] [PubMed]
  16. Rodrigues-Fleming, G.H.; de Mendonça Fernandes, G.M.; Russo, A.; Biselli-Chicote, P.M.; Netinho, J.G.; Pavarino, É.C.; Goloni-Bertollo, E.M. Molecular evaluation of glutathione S transferase family genes in patients with sporadic colorectal cancer. World J. Gastroenterol. 2018, 24, 4462–4471. [Google Scholar] [CrossRef] [PubMed]
  17. Funke, S.; Timofeeva, M.; Risch, A.; Hoffmeister, M.; Stegmaier, C.; Seiler, C.M.; Brenner, H.; Chang-Claude, J. Genetic polymorphisms in GST genes and survival of colorectal cancer patients treated with chemotherapy. Pharmacogenomics 2010, 11, 33–41. [Google Scholar] [CrossRef] [PubMed]
  18. Kap, E.J.; Richter, S.; Rudolph, A.; Jansen, L.; Ulrich, A.; Hoffmeister, M.; Ulrich, C.M.; Brenner, H.; Chang-Claude, J. Genetic variants in the glutathione S-transferase genes and survival in colorectal cancer patients after chemotherapy and differences according to treatment with oxaliplatin. Pharmacogenetics Genom. 2014, 24, 340–347. [Google Scholar] [CrossRef] [PubMed]
  19. Benson, A.B.; Venook, A.P.; Al-Hawary, M.M.; Arain, M.A.; Chen, Y.-J.; Ciombor, K.K.; Cohen, S.; Cooper, H.S.; Deming, D.; Farkas, L.; et al. Colon Cancer, Version 2.2021, NCCN Clinical Practice Guidelines in Oncology. J. Natl. Compr. Cancer Netw. 2021, 19, 329–359. [Google Scholar] [CrossRef]
  20. Liu, J.; Liu, X.; Qian, J.; Meng, C.; Zhu, P.; Hang, J.; Wang, Y.; Xiong, B.; Qiu, X.; Zhu, W.; et al. Development of pH/Glutathione-Responsive Theranostic Agents Activated by Glutathione S-Transferase π for Human Colon Cancer. J. Med. Chem. 2020, 63, 9271–9283. [Google Scholar] [CrossRef] [PubMed]
  21. Kumamoto, K.; Ishibashi, K.; Okada, N.; Tajima, Y.; Kuwabara, K.; Kumagai, Y.; Baba, H.; Haga, N.; Ishida, H. Polymorphisms of GSTP1, ERCC2 and TS-3′UTR are associated with the clinical outcome of mFOLFOX6 in colorectal cancer patients. Oncol. Lett. 2013, 6, 648–654. [Google Scholar] [CrossRef] [PubMed]
  22. Edge, S.B.; Compton, C.C. The American Joint Committee on Cancer: The 7th edition of the AJCC cancer staging manual and the future of TNM. Ann. Surg. Oncol. 2010, 17, 1471–1474. [Google Scholar] [CrossRef] [PubMed]
  23. Tournigand, C.; André, T.; Achille, E.; Lledo, G.; Flesh, M.; Mery-Mignard, D.; Quinaux, E.; Couteau, C.; Buyse, M.; Ganem, G.; et al. FOLFIRI followed by FOLFOX6 or the reverse sequence in advanced colorectal cancer: A randomized GERCOR study. J. Clin. Oncol. 2004, 22, 229–237. [Google Scholar] [CrossRef] [PubMed]
  24. Eisenhauer, E.A.; Therasse, P.; Bogaerts, J.; Schwartz, L.H.; Sargent, D.; Ford, R.; Dancey, J.; Arbuck, S.; Gwyther, S.; Mooney, M.; et al. New response evaluation criteria in solid tumours: Revised RECIST guideline (version 1.1). Eur. J. Cancer 2009, 45, 228–247. [Google Scholar] [CrossRef] [PubMed]
  25. Abdel-Rahman, S.Z.; el-Zein, R.A.; Anwar, W.A.; Au, W.W. A multiplex PCR procedure for polymorphic analysis of GSTM1 and GSTT1 genes in population studies. Cancer Lett. 1996, 107, 229–233. [Google Scholar] [CrossRef] [PubMed]
  26. Ping, J.; Wang, H.; Huang, M.; Liu, Z.-S. Genetic analysis of glutathione S-transferase A1 polymorphism in the Chinese population and the influence of genotype on enzymatic properties. Toxicol. Sci. 2006, 89, 438–443. [Google Scholar] [CrossRef] [PubMed]
  27. Stojkovic Lalosevic, M.L.; Coric, V.M.; Pekmezovic, T.D.; Simic, T.P.; Pljesa Ercegovac, M.S.; Pavlovic Markovic, A.R.; Krivokapic, Z.V. Deletion and Single Nucleotide Polymorphisms in Common Glutathione-S Transferases Contribute to Colorectal Cancer Development. Pathol. Oncol. Res. 2019, 25, 1579–1587. [Google Scholar] [CrossRef] [PubMed]
  28. Zhunussova, G.; Zhunusbekova, B.; Djansugurova, L. Association between glutathione S-transferase M1 and T1 polymorphisms and colorectal cancer risk in patients from Kazakhstan. Clin. Lab. 2015, 61, 161–168. [Google Scholar] [CrossRef] [PubMed]
  29. Klusek, J.; Nasierowska-Guttmejer, A.; Kowalik, A.; Wawrzycka, I.; Chrapek, M.; Lewitowicz, P.; Radowicz-Chil, A.; Klusek, J.; Głuszek, S. The Influence of Red Meat on Colorectal Cancer Occurrence Is Dependent on the Genetic Polymorphisms of S-Glutathione Transferase Genes. Nutrients 2019, 11, 1682. [Google Scholar] [CrossRef] [PubMed]
  30. Allocati, N.; Masulli, M.; Di Ilio, C.; Federici, L. Glutathione transferases: Substrates, inihibitors and pro-drugs in cancer and neurodegenerative diseases. Oncogenesis 2018, 7, 8. [Google Scholar] [CrossRef] [PubMed]
  31. Liu, H.; Shi, W.; Zhao, L.; Dai, D.; Gao, J.; Kong, X. Can GSTM1 and GSTT1 polymorphisms predict clinical outcomes of chemotherapy in gastric and colorectal cancers? A result based on the previous reports. OncoTargets Ther. 2016, 9, 3683–3694. [Google Scholar] [CrossRef]
  32. Huang, M.; Zeng, Y.; Zhao, F.; Huang, Y. Association of glutathione S-transferase M1 polymorphisms in the colorectal cancer risk: A meta-analysis. J. Cancer Res. Ther. 2018, 14, 176–183. [Google Scholar] [CrossRef] [PubMed]
  33. Polimanti, R.; Carboni, C.; Baesso, I.; Piacentini, S.; Iorio, A.; De Stefano, G.F.; Fuciarelli, M. Genetic variability of glutathione S-transferase enzymes in human populations: Functional inter-ethnic differences in detoxification systems. Gene 2013, 512, 102–107. [Google Scholar] [CrossRef]
  34. Csejtei, A.; Tibold, A.; Varga, Z.; Koltai, K.; Ember, A.; Orsos, Z.; Feher, G.; Horvath, O.P.; Ember, I.; Kiss, I. GSTM, GSTT and p53 polymorphisms as modifiers of clinical outcome in colorectal cancer. Anticancer Res. 2008, 28, 1917–1922. [Google Scholar] [PubMed]
  35. Feng, Z.; Liu, Z.; Peng, K.; Wu, W. A Prognostic Model Based on Nine DNA Methylation-Driven Genes Predicts Overall Survival for Colorectal Cancer. Front. Genet. 2021, 12, 779383. [Google Scholar] [CrossRef] [PubMed]
  36. Liao, C.; Cao, Y.; Wu, L.; Huang, J.; Gao, F. An updating meta-analysis of the glutathione S-transferase T1 polymorphisms and colorectal cancer risk: A HuGE review. Int. J. Color. Dis. 2010, 25, 25–37. [Google Scholar] [CrossRef] [PubMed]
  37. Martínez, C.; Martín, F.; Fernández, J.M.; García-Martín, E.; Sastre, J.; Díaz-Rubio, M.; Agúndez, J.A.; Ladero, J.M. Glutathione S-transferases mu 1, theta 1, pi 1, alpha 1 and mu 3 genetic polymorphisms and the risk of colorectal and gastric cancers in humans. Pharmacogenomics 2006, 7, 711–718. [Google Scholar] [CrossRef] [PubMed]
  38. Doğru-Abbasoğlu, S.; Mutlu-Türkoğlu, U.; Türkoğlu, S.; Erbil, Y.; Barbaros, U.; Uysal, M.; Aykaç-Toker, G. Glutathione S-transferase-pi in malignant tissues and plasma of human colorectal and gastric cancers. J. Cancer Res. Clin. Oncol. 2002, 128, 91–95. [Google Scholar] [CrossRef]
  39. Sau, A.; Pellizzari Tregno, F.; Valentino, F.; Federici, G.; Caccuri, A.M. Glutathione transferases and development of new principles to overcome drug resistance. Arch. Biochem. Biophys. 2010, 500, 116–122. [Google Scholar] [CrossRef] [PubMed]
  40. Mehrzad, V.; Roayaei, M.; Peikar, M.S.; Nouranian, E.; Mokarian, F.; Khani, M.; Farzannia, S. Bevacizumab plus FOLFOX or FOLFIRI regimens on patients with unresectable liver-only metastases of metastatic colorectal cancer. Adv. Biomed. Res. 2016, 5, 10. [Google Scholar] [CrossRef]
  41. Shahnam, A.; Ridha, Z.; Wiese, M.D.; Kichenadasse, G.; Sorich, M.J. Pharmacogenetic and ethnicity influence on oxaliplatin therapy for colorectal cancer: A meta-analysis. Pharmacogenomics 2016, 17, 1725–1732. [Google Scholar] [CrossRef] [PubMed]
  42. McLeod, H.L.; Sargent, D.J.; Marsh, S.; Green, E.M.; King, C.R.; Fuchs, C.S.; Ramanathan, R.K.; Williamson, S.K.; Findlay, B.P.; Thibodeau, S.N.; et al. Pharmacogenetic Predictors of Adverse Events and Response to Chemotherapy in Metastatic Colorectal Cancer: Results From North American Gastrointestinal Intergroup Trial N9741. J. Clin. Oncol. 2010, 28, 3227–3233. [Google Scholar] [CrossRef] [PubMed]
  43. Boige, V.; Mendiboure, J.; Pignon, J.-P.; Loriot, M.-A.; Castaing, M.; Barrois, M.; Malka, D.; Trégouët, D.-A.; Bouché, O.; Le Corre, D.; et al. Pharmacogenetic assessment of toxicity and outcome in patients with metastatic colorectal cancer treated with LV5FU2, FOLFOX, and FOLFIRI: FFCD 2000-05. J. Clin. Oncol. 2010, 28, 2556–2564. [Google Scholar] [CrossRef] [PubMed]
  44. Stoehlmacher, J.; Park, D.J.; Zhang, W.; Yang, D.; Groshen, S.; Zahedy, S.; Lenz, H.-J. A multivariate analysis of genomic polymorphisms: Prediction of clinical outcome to 5-FU/oxaliplatin combination chemotherapy in refractory colorectal cancer. Br. J. Cancer 2004, 91, 344–354. [Google Scholar] [CrossRef] [PubMed]
  45. Pljesa-Ercegovac, M.; Savic-Radojevic, A.; Matic, M.; Coric, V.; Djukic, T.; Radic, T.; Simic, T. Glutathione Transferases: Potential Targets to Overcome Chemoresistance in Solid Tumors. Int. J. Mol. Sci. 2018, 19, 3785. [Google Scholar] [CrossRef]
  46. Thévenin, A.F.; Zony, C.L.; Bahnson, B.J.; Colman, R.F. GST pi modulates JNK activity through a direct interaction with JNK substrate, ATF2. Protein Sci. 2011, 20, 834–848. [Google Scholar] [CrossRef] [PubMed]
Medicina 60 00553 g001aMedicina 60 00553 g001b
Figure 1. Overall survival of individuals with CRC with respect to different GST genotypes (a) Kaplan–Meier survival curves according to GSTA1 polymorphism; Low activity if one active allele is present; (b) Kaplan–Meier survival curves according to GSTM1 polymorphism; Active if one active allele is present; Null if no active allele is present (c) Kaplan–Meier survival curves according to GSTT1 polymorphism; Active if one active allele is present; Null if no active allele is present (d) Kaplan–Meier survival curves according to GSTP1 polymorphism; Variant if one Val allele is present.
Figure 1. Overall survival of individuals with CRC with respect to different GST genotypes (a) Kaplan–Meier survival curves according to GSTA1 polymorphism; Low activity if one active allele is present; (b) Kaplan–Meier survival curves according to GSTM1 polymorphism; Active if one active allele is present; Null if no active allele is present (c) Kaplan–Meier survival curves according to GSTT1 polymorphism; Active if one active allele is present; Null if no active allele is present (d) Kaplan–Meier survival curves according to GSTP1 polymorphism; Variant if one Val allele is present.
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Figure 2. Effects of GST polymorphisms on overall survival of CRC patients on 5-FU-based treatment. (a) Kaplan–Meier survival curves according to GSTA1 polymorphism; Low activity if one active allele is present; (b) Kaplan–Meier survival curves according to GSTM1 polymorphism; Active if one active allele is present; Null if no active allele is present (c) Kaplan–Meier survival curves according to GSTT1 polymorphism; Active if one active allele is present; Null if no active allele is present; (d) Kaplan–Meier survival curves according to GSTP1 polymorphism; Variant if one Val allele is present.
Figure 2. Effects of GST polymorphisms on overall survival of CRC patients on 5-FU-based treatment. (a) Kaplan–Meier survival curves according to GSTA1 polymorphism; Low activity if one active allele is present; (b) Kaplan–Meier survival curves according to GSTM1 polymorphism; Active if one active allele is present; Null if no active allele is present (c) Kaplan–Meier survival curves according to GSTT1 polymorphism; Active if one active allele is present; Null if no active allele is present; (d) Kaplan–Meier survival curves according to GSTP1 polymorphism; Variant if one Val allele is present.
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Table 1. Available patient clinical characteristics.
Table 1. Available patient clinical characteristics.
Living, n%Deceased, n%p-Value
Sex
Male203 (70)86 (30)0.235
Female137 (75)45 (25)
Age (mean ± SD)62.42 ± 10.4762.71 ± 13.090.815
Localization
Left side65 (78)23 (22)0.697
Right side275 (73)108 (27)
TNM
I123 (93)9 (7)0.001
II106 (88)14 (12)
III86 (58)62 (42)
IV25 (36)46 (64)
Grade
Well differentiated267 (76)84 (24)0.005
Moderately differentiated61 (60)38 (40)
Poorly differentiated12 (57)9 (43)
Table 2. GST genotype distribution in living and deceased patients with colorectal cancer.
Table 2. GST genotype distribution in living and deceased patients with colorectal cancer.
GST GenotypeLiving, n%Deceased, n%p-Value
GSTM1
active a182 (80)47 (20)0.001
null b150 (66)79 (34)
GSTT1
null b89 (69)41 (31)0.246
active a243 (74)89 (26)
GSTA1 (rs 3957357)
CC (active)120 (73)44 (27)0.913
CT+TT (low activity) c215 (72)82 (28)
GSTP1 (rs1695)
IleIle (referent)139(80)35 (20)0.009
IleVal+ValVal (variant) d185 (68)86 (32)
a Active, at least one allele present b Null, none of the alleles are present; c Variant, at least one Val allele present; d Low activity at least one T allele present; the data represent the number of successful genotypisations.
Table 3. The prognostic effect of GST genotypes on the risk of overall mortality in CRC patients (Model 1).
Table 3. The prognostic effect of GST genotypes on the risk of overall mortality in CRC patients (Model 1).
Model 1
HR (95% CI)p-Value
Risk of mortality in carriers of GSTM1-null a genotype
compared to carriers of GSTM1-active b genotype
1.81 (1.22–2.68)0.003
Risk of mortality in carriers of GSTT1-null a genotype
compared to carriers of GSTT1-active b genotype
1.24 (0.82–1.87)0.301
Risk of mortality in carriers of GSTA1-low activity c genotype
compared to carriers of GSTA1-active b genotype
1.03 (0.69–1.53)0.873
Risk of mortality in carriers of GSTP1-variant d genotype
compared to carriers of GSTP1-referent genotype
1.53 (0.67–2.46)0.048
Model 1 is adjusted to age and sex; a Active, if at least one active allele is present; b Null if no active alleles are present; c Low activity, if at least one T allele is present. d Variant, if at least one Val allele is present; HR, hazard ratio; CI, confidence interval.
Table 4. The prognostic effect of GST genotypes on the risk of overall mortality in CRC patients (Model 2).
Table 4. The prognostic effect of GST genotypes on the risk of overall mortality in CRC patients (Model 2).
Model 2
HR (95% CI)p-Value
Risk of mortality in carriers of GSTM1-null a genotype
compared to carriers of GSTM1-active b genotype
1.79 (1.21–2.65)0.004
Risk of mortality in carriers of GSTT1-null a genotype
compared to carriers of GSTT1-active b genotype
1.24 (0.82–1.88)0.300
Risk of mortality in carriers of GSTA1-low activity c genotype
compared to carriers of GSTA1-active b genotype
1.00 (0.67–1.50)0.986
Risk of mortality in carriers of GSTP1-variant d genotype
compared to carriers of GSTP1-referent genotype
1.57 (1.03–2.39)0.035
Model 2 is adjusted to the covariates of Model 1 and known CRC risk factors (smoking, BMI); a Active, if at least one active allele is present; b Null if no active alleles are present; c Low activity, if at least one T allele is present. d Variant, if at least one Val allele is present; HR, hazard ratio; CI, confidence interval.
Table 5. The prognostic effect of GST genotypes on the risk of overall mortality in CRC patients (Model 3).
Table 5. The prognostic effect of GST genotypes on the risk of overall mortality in CRC patients (Model 3).
Model 3
HR (95% CI)p-Value
Risk of mortality in carriers of GSTM1-null a genotype
compared to carriers of GSTM1-active b genotype
1.89 (1.21–2.65)0.001
Risk of mortality in carriers of GSTT1-null a genotype
compared to carriers of GSTT1-active b genotype
1.25 (0.85–1.84)0.250
Risk of mortality in carriers of GSTA1-low activity c genotype
compared to carriers of GSTA1-active b genotype
1.02 (0.70–1.48)0.918
Risk of mortality in carriers of GSTP1-variant d genotype
compared to carriers of GSTP1-referent genotype
1.47 (0.99–2.20)0.050
Model 3 is adjusted to covariates of Model 2 and FOLFOX6 treatment; a Active, if at least one active allele present; b Null if no active alleles present; c Low activity, if at least one T allele present. d Variant, if at least one Val allele present; HR, hazard ratio; CI, confidence interval.
Table 6. The prognostic effect of GST genotypes on the risk of overall mortality in CRC patients (Model 4).
Table 6. The prognostic effect of GST genotypes on the risk of overall mortality in CRC patients (Model 4).
Model 4
HR (95% CI)p-Value
Risk of mortality in carriers GSTM1-null a genotype
compared to carriers of GSTM1-active b genotype
1.89 (1.31–2.74)0.001
Risk of mortality in carriers GSTT1-null a genotype
compared to carriers of GSTT1-active b genotype
1.26 (0.86–1.84)0.245
Risk of mortality in carriers GSTA1-low activity c genotype
compared to carriers of GSTA1-active b genotype
1.02 (0.70–1.49)0.902
Risk of mortality in carriers GSTP1-variant d genotype
compared to carriers of GSTP1-referent genotype
1.50 (1.01–2.24)0.046
Model 4 is adjusted to covariates of tumor localization and differentiation; a Active, if at least one active allele is present; b Null if no active alleles are present; c Low activity, if at least one T allele is present. d Variant, if at least one Val allele is present; HR, hazard ratio; CI, confidence interval.
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MDPI and ACS Style

Stojkovic Lalosevic, M.; Coric, V.; Pekmezovic, T.; Simic, T.; Pavlovic Markovic, A.; Pljesa Ercegovac, M. GSTM1 and GSTP1 Polymorphisms Affect Outcome in Colorectal Adenocarcinoma. Medicina 2024, 60, 553. https://doi.org/10.3390/medicina60040553

AMA Style

Stojkovic Lalosevic M, Coric V, Pekmezovic T, Simic T, Pavlovic Markovic A, Pljesa Ercegovac M. GSTM1 and GSTP1 Polymorphisms Affect Outcome in Colorectal Adenocarcinoma. Medicina. 2024; 60(4):553. https://doi.org/10.3390/medicina60040553

Chicago/Turabian Style

Stojkovic Lalosevic, Milica, Vesna Coric, Tatjana Pekmezovic, Tatjana Simic, Aleksandra Pavlovic Markovic, and Marija Pljesa Ercegovac. 2024. "GSTM1 and GSTP1 Polymorphisms Affect Outcome in Colorectal Adenocarcinoma" Medicina 60, no. 4: 553. https://doi.org/10.3390/medicina60040553

APA Style

Stojkovic Lalosevic, M., Coric, V., Pekmezovic, T., Simic, T., Pavlovic Markovic, A., & Pljesa Ercegovac, M. (2024). GSTM1 and GSTP1 Polymorphisms Affect Outcome in Colorectal Adenocarcinoma. Medicina, 60(4), 553. https://doi.org/10.3390/medicina60040553

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