Low Calcium–High Magnesium Krebs–Henseleit Solution Combined with Adenosine and Lidocaine Improved Rat Aortic Function and Structure Following Cold Preservation
Abstract
:1. Introduction
2. Materials and Methods
2.1. Chemicals
2.2. Preservation Solution Compositions
- Krebs–Henseleit (KH): 118 mM NaCl, 4.7 mM KCl, 1.2 mM NaH2PO4, 2.5 mM CaCl2, 1.2 mM MgCl2, 25 mM NaHCO3, 0.03 mM Na-EDTA, and 11 mM glucose.
- Modified KH: 118 mM NaCl, 4.7 mM KCl, 1.2 mM NaH2PO4, 0.22 mM CaCl2, 2.6 mM MgCl2, 25 mM NaHCO3, 0.03 mM Na-EDTA, and 11 mM glucose.
- Modified KH + AL: modified KH, 0.4 mM adenosine, and 1 mM lidocaine.
- Modified KH +ALMI: modified KH, 0.4 mM adenosine, 1 mM lidocaine, 0.1 mM melatonin, and 0.01 IU/mL insulin.
2.3. Animal Preparation
2.4. Aortic Ring Preparation
2.5. Vessel Function Analysis
2.6. Vessel Histopathological Analysis
2.7. Statistical Analysis
3. Results
3.1. NE- and KCl-Induced Aortic Vasoconstrictive Responses with Different Preservation Solutions
3.2. Ach- and NO-Induced Aortic Vasorelaxation Responses in Different Preservation Solutions
3.3. Maximum Aortic Relaxation Function after 6 Days of Storage in Preservation Solution Compared with Freshly Harvested Aortic Rings
3.4. Vessel Histopathological Analysis after 6 Days of Storage in Preservation Solution
4. Discussion
Limitation of the Study
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Groups | Elastic Membrane Deterioration | Denudation of Endothelial Cells |
---|---|---|
Control | 0 | 0 |
Standard KH | 2 | 2 |
Mod KH | 1 | 1 |
Mod KH-AL | 0 | 0 |
Mod KH-ALMI | 0 | 0 |
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Arsyad, A.; Lembang, G.K.R.; Linda, S.L.; Djabir, Y.Y.; Dobson, G.P. Low Calcium–High Magnesium Krebs–Henseleit Solution Combined with Adenosine and Lidocaine Improved Rat Aortic Function and Structure Following Cold Preservation. Medicina 2024, 60, 1284. https://doi.org/10.3390/medicina60081284
Arsyad A, Lembang GKR, Linda SL, Djabir YY, Dobson GP. Low Calcium–High Magnesium Krebs–Henseleit Solution Combined with Adenosine and Lidocaine Improved Rat Aortic Function and Structure Following Cold Preservation. Medicina. 2024; 60(8):1284. https://doi.org/10.3390/medicina60081284
Chicago/Turabian StyleArsyad, Aryadi, Geni K. R. Lembang, Sesilia L. Linda, Yulia Y. Djabir, and Geoffrey P. Dobson. 2024. "Low Calcium–High Magnesium Krebs–Henseleit Solution Combined with Adenosine and Lidocaine Improved Rat Aortic Function and Structure Following Cold Preservation" Medicina 60, no. 8: 1284. https://doi.org/10.3390/medicina60081284