Effects of Different No-Ozone Cold Plasma Treatment Methods on Mouse Osteoblast Proliferation and Differentiation
Abstract
:1. Introduction
2. Materials and Methods
2.1. NCP Generation
2.2. Cell Culture
2.3. NCP Treatment
- First group: not treated with NCP (NT);
- Second group: treated directly with NCP in the dish with cells (DT);
- Third group: treated directly with NCP in the dish, and the medium was immediately replaced (MC);
- Fourth group: treated with NCP in a 35 mm dish without cells for 1 min, and then transferred to the dish with cells (PAM).
2.4. Water-Soluble Tetrazolium 1 (WST-1) Salt Assay
2.5. Alkaline Phosphatase (ALP) Staining
2.6. ALP Activity
2.7. Alizarin Red S Staining
2.8. Real-Time Polymerase Chain Reaction (qPCR)
2.9. Statistical Analysis
3. Results
3.1. Effect of NCP on MC3T3-E1 Cell Proliferation
3.2. ALP Activity in the NCP-Treated MC3T3-E1 Cells According to NCP Treatment Method
3.3. Calcium Deposition in NCP-Treated MC3T3-E1 Cells
3.4. mRNA Expression Changes in NCP-Treated MC3T3-E1 Cells
4. Discussion
5. Conclusions
6. Patents
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
- Cai, W.; Sun, B.; Song, C.; Liu, F.; Wu, Z.; Liu, Z. Resveratrol induces proliferation and differentiation of mouse pre-osteoblast MC3T3-E1 by promoting autophagy. BMC Complement. Med. Ther. 2023, 23, 121. [Google Scholar] [CrossRef] [PubMed]
- What is Osteoporosis? Available online: https://www.osteoporosis.foundation/patients/about-osteoporosis (accessed on 1 May 2024).
- Xiao, Y.; Xie, X.; Chen, Z.; Yin, G.; Kong, W.; Zhou, J. Advances in the roles of ATF4 in osteoporosis. Biomed. Pharmacother. 2023, 169, 115864. [Google Scholar] [CrossRef] [PubMed]
- Blair, H.C.; Larrouture, Q.C.; Li, Y.; Lin, H.; Beer-Stoltz, D.; Liu, L.; Tuan, R.S.; Robinson, L.J.; Schlesinger, P.H.; Nelson, D.J. Osteoblast Differentiation and Bone Matrix Formation In Vivo and In Vitro. Tissue Eng. Part B Rev. 2017, 23, 268–280. [Google Scholar] [CrossRef] [PubMed]
- Kim, J.L.; Park, S.H.; Jeong, D.; Nam, J.S.; Kang, Y.H. Osteogenic activity of silymarin through enhancement of alkaline phosphatase and osteocalcin in osteoblasts and tibia-fractured mice. Exp. Biol. Med. 2012, 237, 417–428. [Google Scholar] [CrossRef]
- Feng, X.; McDonald, J.M. Disorders of bone remodeling. Annu. Rev. Pathol. Mech. Dis. 2011, 6, 121–145. [Google Scholar] [CrossRef]
- Li, W.; Zhang, S.; Liu, J.; Liu, Y.; Liang, Q. Vitamin K2 stimulates MC3T3-E1 osteoblast differentiation and mineralization through autophagy induction. Mol. Med. Rep. 2019, 19, 3676–3684. [Google Scholar] [CrossRef]
- Khan, A.A.; Sandor, G.K.; Dore, E.; Morrison, A.D.; Alsahli, M.; Amin, F.; Peters, E.; Hanley, D.A.; Chaudry, S.R.; Lentle, B. Bisphosphonate associated osteonecrosis of the jaw. J. Rheumatol. 2009, 36, 478–490. [Google Scholar] [CrossRef] [PubMed]
- Watts, N.B.; Diab, D.L. Long-term use of bisphosphonates in osteoporosis. J. Clin. Endocrinol. Metab. 2010, 95, 1555–1565. [Google Scholar] [CrossRef] [PubMed]
- Da Silva, D.; Crous, A.; Abrahamse, H. Enhancing Osteoblast Differentiation from Adipose-Derived Stem Cells Using Hydrogels and Photobiomodulation: Overcoming in Vitro Limitations for Osteoporosis Treatment. Curr. Issues Mol. Biol. 2024, 46, 6346–6365. [Google Scholar] [CrossRef]
- Tanaka, H.; Hori, M. Medical applications of non-thermal atmospheric pressure plasma. J. Clin. Biochem. Nutr. 2017, 60, 29–32. [Google Scholar] [CrossRef]
- Yahaya, A.G.; Okuyama, T.; Kristof, J.; Blajan, M.G.; Shimizu, K. Direct and Indirect Bactericidal Effects of Cold Atmospheric-Pressure Microplasma and Plasma Jet. Molecules 2021, 26, 2523. [Google Scholar] [CrossRef]
- Park, S.Y.; Jaiswal, M.S.; Jang, Y.S.; Choi, J.H.; Kim, G.C.; Hong, J.W.; Hwang, D.S. Effect of No-ozone Cold Plasma on Regeneration after Crushed Mental Nerve Injury in rats. Int. J. Med. Sci. 2022, 25, 1732–1742. [Google Scholar] [CrossRef] [PubMed]
- Faramarzi, F.; Zafari, P.; Alimohammadi, M.; Moonesi, M.; Rafiei, A.; Bekeschus, S. Cold Physical Plasma in Cancer Therapy: Mechanisms, Signaling, and Immunity. Oxidative Med. Cell. Longev. 2021, 24, 9916796. [Google Scholar] [CrossRef]
- Borges, A.C.; Kostov, K.G.; Pessoa, R.S.; de Abreu, G.M.A.; Lima, G.d.M.G.; Figueira, L.W.; Koga-Ito, C.Y. Applications of Cold Atmospheric Pressure Plasma in Dentistry. Appl. Sci. 2021, 11, 1975. [Google Scholar] [CrossRef]
- Eggers, B.; Wagenheim, A.-M.; Jung, S.; Kleinheinz, J.; Nokhbehsaim, M.; Kramer, F.-J.; Sielker, S. Effect of Cold Atmospheric Plasma (CAP) on Osteogenic Differentiation Potential of Human Osteoblasts. Int. J. Mol. Sci. 2022, 23, 2503. [Google Scholar] [CrossRef]
- Choi, B.-B.; Choi, J.-H.; Kang, T.-H.; Lee, S.-J.; Kim, G.-C. Enhancement of Osteoblast Differentiation Using No-Ozone Cold Plasma on Human Periodontal Ligament Cells. Biomedicines 2021, 9, 1542. [Google Scholar] [CrossRef]
- Graves, D.B. The emerging role of reactive oxygen and nitrogen species in redox biology and some implications for plasma applications to medicine and biology. J. Phys. D Appl. Phys. 2012, 45, 263001. [Google Scholar] [CrossRef]
- Denoya, G.I.; Szerman, N.; Vaudagna, S.R. Application of Direct and Indirect Non-thermal Plasma in the Development of Ready-to-Eat Foods. Curr. Food Sci. Technol. Rep. 2024, 2, 45–54. [Google Scholar] [CrossRef]
- Fridman, G.; Fridman, A.; Gutsol, A.; Vasilets, V.; Friedman, G. Comparison of Direct and Indirect Effects of Non-Thermal Atmospheric Pressure Plasma on Bacteria and Mechanisms of Such Interaction. In Proceedings of the IEEE 34th International Conference on Plasma Science (ICOPS), Albuquerque, NM, USA, 17–22 June 2007; p. 322. [Google Scholar]
- Liu, L.; Zeng, L.; Gao, L.; Zeng, J.; Lu, J. Ozone therapy for skin diseases: Cellular and molecular mechanisms. Int. Wound J. 2023, 20, 2376–2385. [Google Scholar] [CrossRef]
- Oliveira Modena, D.A.; de Castro Ferreira, R.; Froes, P.M.; Rocha, K.C. Ozone Therapy for Dermatological Conditions: A Systematic Review. J. Clin. Aesthet. Dermatol. 2022, 15, 65–73. [Google Scholar]
- Saini, R. Ozone therapy in dentistry: A strategic review. J. Nat. Sci. Biol. Med. 2011, 2, 151–153. [Google Scholar] [CrossRef] [PubMed]
- Baeza-Noci, J.; Pinto-Bonilla, R. Systemic Review: Ozone: A Potential New Chemotherapy. Int. J. Mol. Sci. 2021, 22, 11796. [Google Scholar] [CrossRef] [PubMed]
- Brown, J.S.; Bateson, T.F.; McDonnell, W.F. Effects of exposure to 0.06 ppm ozone on FEV1 in humans: A secondary analysis of existing data. Environ. Health Perspect. 2008, 116, 1023–1026. [Google Scholar] [CrossRef] [PubMed]
- Ozone Generators that are Sold as Air Cleaners. Available online: https://www.epa.gov/indoor-air-quality-iaq/ozone-generators-are-sold-air-cleaners (accessed on 1 May 2024).
- Yin, L.M.; Wei, Y.; Wang, Y.; Xu, Y.D.; Yang, Y.Q. Long term and standard incubations of WST-1 reagent reflect the same inhibitory trend of cell viability in rat airway smooth muscle cells. Int. J. Med. Sci. 2013, 10, 68–72. [Google Scholar] [CrossRef] [PubMed]
- Lee, J.M.; Kim, M.G.; Byun, J.H.; Kim, G.C.; Ro, J.H.; Hwang, D.S.; Choi, B.B.; Park, G.C.; Kim, U.K. The effect of biomechanical stimulation on osteoblast differentiation of human jaw periosteum-derived stem cells. Maxillofac. Plast. Reconstr. Surg. 2017, 39, 7. [Google Scholar] [CrossRef] [PubMed]
- Chai, X.; Zhang, W.; Chang, B.; Feng, X.; Song, J.; Li, L.; Yu, C.; Zhao, J.; Si, H. GPR39 agonist TC-G 1008 promotes osteoblast differentiation and mineralization in MC3T3-E1 cells. Artif. Cells Nanomed. Biotechnol. 2019, 47, 3569–3576. [Google Scholar] [CrossRef]
- Escobar, L.M.; Bendahan, Z.; Garcia, C.; Castellanos, J.E. Relaxin treatment stimulates the differentiation of mesenchymal stem cells into osteoblasts. J. Dent. Sci. 2023, 18, 1786–1793. [Google Scholar] [CrossRef] [PubMed]
- Al-rawaf, A.F.; Fuliful, F.K.; Khalaf, M.K.; Oudah, H.K. Studying the non-thermal plasma jet characteristics and application on bacterial decontamination. J. Theor. Appl. Phys. 2018, 12, 45–51. [Google Scholar] [CrossRef]
- Kang, K.A.; Piao, M.J.; Eom, S.; Yoon, S.Y.; Ryu, S.; Kim, S.B.; Yi, J.M.; Hyun, J.W. Non-thermal dielectric-barrier discharge plasma induces reactive oxygen species by epigenetically modifying the expression of NADPH oxidase family genes in keratinocytes. Redox Biol. 2020, 37, 101698. [Google Scholar] [CrossRef] [PubMed]
- Yang, Y.; Wang, Y.; Wei, S.; Wang, X.; Zhang, J. Effects and Mechanisms of Non-Thermal Plasma-Mediated ROS and Its Applications in Animal Husbandry and Biomedicine. Int. J. Mol. Sci. 2023, 24, 15889. [Google Scholar] [CrossRef]
- Lee, H.Y.; Choi, J.H.; Hong, J.W.; Kim, G.C.; Lee, H.J. Comparative study of the Ar and He atmospheric pressure plasmas on E-cadherin protein regulation for plasma-mediated transdermal drug delivery. J. Phys. D Appl. Phys. 2018, 51, 215401. [Google Scholar] [CrossRef]
- Park, N.S.; Yun, S.E.; Lee, H.Y.; Lee, H.J.; Choi, J.H.; Kim, G.C. No-ozone cold plasma can kill oral pathogenic microbes in H2O2-dependent and independent manner. Sci. Rep. 2022, 12, 7597. [Google Scholar] [CrossRef] [PubMed]
- Tabassum, A.; Wismeijer, D.; Hogervorst, J.; Tahmaseb, A. Comparison of Proliferation and Differentiation of Human Osteoblast-like Cells Harvested During Implant Osteotomy Preparation Using Two Different Drilling Protocols. Int. J. Oral Maxillofac. Implant. 2020, 35, 141–149. [Google Scholar] [CrossRef] [PubMed]
- Cao, T.; Heng, B.C.; Ye, C.P.; Liu, H.; Toh, W.S.; Robson, P.; Li, P.; Hong, Y.H.; Stanton, L.W. Osteogenic differentiation within intact human embryoid bodies result in a marked increase in osteocalcin secretion after 12 days of in vitro culture, and formation of morphologically distinct nodule-like structures. Tissue Cell 2005, 37, 325–334. [Google Scholar] [CrossRef]
- Park, B.W.; Hah, Y.S.; Kim, D.R.; Kim, J.R.; Byun, J.H. Osteogenic phenotypes and mineralization of cultured human periosteal-derived cells. Arch. Oral Biol. 2007, 52, 983–989. [Google Scholar] [CrossRef]
- Tsai, M.T.; Lin, D.J.; Huang, S.; Lin, H.T.; Chang, W.H. Osteogenic differentiation is synergistically influenced by osteoinductive treatment and direct cell-cell contact between murine osteoblasts and mesenchymal stem cells. Int. Orthop. 2012, 36, 199–205. [Google Scholar] [CrossRef] [PubMed]
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Choi, B.-B.R.; Park, S.-R.; Kim, G.-C. Effects of Different No-Ozone Cold Plasma Treatment Methods on Mouse Osteoblast Proliferation and Differentiation. Medicina 2024, 60, 1318. https://doi.org/10.3390/medicina60081318
Choi B-BR, Park S-R, Kim G-C. Effects of Different No-Ozone Cold Plasma Treatment Methods on Mouse Osteoblast Proliferation and Differentiation. Medicina. 2024; 60(8):1318. https://doi.org/10.3390/medicina60081318
Chicago/Turabian StyleChoi, Byul-Bo Ra, Sang-Rye Park, and Gyoo-Cheon Kim. 2024. "Effects of Different No-Ozone Cold Plasma Treatment Methods on Mouse Osteoblast Proliferation and Differentiation" Medicina 60, no. 8: 1318. https://doi.org/10.3390/medicina60081318