Birth of Thirty-Two Healthy Babies Following Transfer of Fresh and Frozen–Thawed Embryos Derived from Monopronuclear Zygotes: A Retrospective Study
Abstract
:1. Introduction
2. Materials and Methods
2.1. Study Design and Patients
2.2. Ovarian Stimulation and Insemination
2.3. Embryo Culture, Evaluation, Transfer and Cryopreservation
2.4. Embryo Thawing
3. Results
3.1. Study Population
3.2. Embryo Outcomes
3.3. Pregnancy and Clinical Outcomes of Cleaved 1PN Embryos in Fresh and Frozen–Thawed Cycles
3.4. Neonatal Outcome of Cleaved 1PN Embryos in Fresh and Frozen–Thawed Cycles
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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cIVF n = 321 Patients | ICSI n = 410 Patients | p Value | |
---|---|---|---|
Age | 36.15 ± 4.6 | 34.36 ± 5.3 | p < 0.001 |
BMI | 24.3 ± 4.1 | 25.3 ± 5 | p < 0.01 |
Cycle rank | 1.2 ± 0.5 | 1.6 ± 1.1 | p < 0.001 |
No of retrieved oocytes | 12 ± 7.6 | 11.4 ± 7.8 | NS |
Serum [E2] pg/mL | 2104 ± 1431 | 2255 ± 1875 | NS |
Serum [P4] ng/mL | 0.75 ± 0.51 | 0.75 ± 0.5 | NS |
Serum [LH] mUI/mL | 1.9 ± 1.9 | 2.6 ± 3.4 | p < 0.001 |
cIVF n = 79 Patients | ICSI n = 71 Patients | p Value | |
---|---|---|---|
Age | 36.1 ± 5 | 34.7 ± 5.8 | NS |
BMI | 24.6 ± 11.8 | 25.5 ± 11.9 | NS |
Fresh cycle rank * | 1.2 ± 0.6 | 2.2 ± 1.5 | p < 0.01 |
No of retrieved oocytes | 15.3 ± 9 | 12.3 ± 8.6 | p < 0.05 |
Embryo transfer rank ** | 2.2 ± 1.5 | 2.2 ± 1.4 | NS |
cFIV n = 648 1PN Zygotes | ICSI n = 586 1PN Zygotes | p Value | |
---|---|---|---|
Usable embryos (cleavage stage and D5–6) | 300/648 (46.3%) | 196/586 (33.4%) | p < 0.001 |
Score A | 103/300 (34.3%) | 67/196 (34.2%) | NS |
Score B | 90/300 (30%) | 45/196 (22.9%) | NS |
Score C | 107/300 (35.7%) | 84/196 (42.9%) | NS |
Transferred embryos | 52/648 (8%) | 55/586 (9.4%) | NS |
Discarded embryos | 348/648 (53.7%) | 390/586 (66.6%) | p < 0.001 |
Blastulation rate | 195/439 (44.4%) | 66/329 (20.1%) | p < 0.001 |
Fresh cIVF (n = 32) | Fresh ICSI (n = 32) | Frozen–Thawed Cycles cIVF (n = 92) | Frozen–Thawed Cycles ICSI (n = 75) | |||||
---|---|---|---|---|---|---|---|---|
1PN Fresh (n = 32) | 2PN FET (n = 17) | 1PN Fresh (n = 32) | 2PN FET (n = 13) | 1PN FET (n = 92) | 2PN FET (n = 118) | 1PN FET (n = 75) | 2PN FET (n = 100) | |
Pregnancy rate | 7/32 (21.9%) | 4/17 (24%) | 4/32 (12.5%) | 7/13 (54%) * | 24/92 (26.1%) | 27/118 (23%) | 12/75 (16%) | 27/100 (27%) |
Miscarriage rate | 2/7 (28.6%) | 1/4 (25%) | 3/4 (75%) | 5/7 (71%) | 7/24 (29.2%) | 13/27 (48%) | 4/12 (33.3%) | 11/27 (41%) |
Live birth rate | 5/32 (15.6%) | 3/17 (18%) | 1/32 (3.1%) | 2/13 (15%) | 17/92 (18.5%) | 14/118 (12%) | 8/75 (10.7%) | 15/100 (15%) |
Fresh cIVF | Fresh ICSI | Frozen–Thawed cIVF Cycles | Frozen–Thawed ICSI Cycles | |||||
---|---|---|---|---|---|---|---|---|
1PN Fresh (n = 5) | 2PN FET (n = 3) | 1PN Fresh (n = 1) | 2PN FET (n = 3) ● | 1PN FET (n = 18) | 2PN FET (n = 14) | 1PN FET (n = 8) | 2PN FET (n = 16) ●● | |
Weeks of gestation | 38 ± 1.5 | 38.5 ± 0.5 | 39.5 | 36.2 ± 2.9 | 37.8 ± 2.7 | 38.5 ± 1.4 | 38.3 ± 1.5 | 38.7 ± 1.6 |
Gender | 2♀/3♂ | 1♀/2♂ | 1♂ | 1♀/2♂ | 10♀/8♂ | 8♀/6♂ | 3♀/5♂ | 10♀/6♂ |
Birth weight (g) | 2743 ± 478 | 4205 ± 398 | 2950 | 2611 ± 1072 | 3336 ± 564 | 3336 ± 524 | 3581 ± 341 | 3259 ± 508 |
Average length (cm) | 46.9 ± 2.8 | 52.7 ± 1.5 | 49 | 47.5 ± 3.8 | 50 ± 3 | 50 ± 2.2 | 48 ± 6 | 45.6 ± 13 |
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Labied, S.; Wenders, F.; Gaspard, O.; Ravet, S.; Desmecht, A.; Nisolle, M.; Henry, L. Birth of Thirty-Two Healthy Babies Following Transfer of Fresh and Frozen–Thawed Embryos Derived from Monopronuclear Zygotes: A Retrospective Study. Medicina 2024, 60, 1361. https://doi.org/10.3390/medicina60081361
Labied S, Wenders F, Gaspard O, Ravet S, Desmecht A, Nisolle M, Henry L. Birth of Thirty-Two Healthy Babies Following Transfer of Fresh and Frozen–Thawed Embryos Derived from Monopronuclear Zygotes: A Retrospective Study. Medicina. 2024; 60(8):1361. https://doi.org/10.3390/medicina60081361
Chicago/Turabian StyleLabied, Soraya, Frédéric Wenders, Olivier Gaspard, Stéphanie Ravet, Alice Desmecht, Michelle Nisolle, and Laurie Henry. 2024. "Birth of Thirty-Two Healthy Babies Following Transfer of Fresh and Frozen–Thawed Embryos Derived from Monopronuclear Zygotes: A Retrospective Study" Medicina 60, no. 8: 1361. https://doi.org/10.3390/medicina60081361