Bioactive Glial-Derived Neurotrophic Factor from a Safe Injectable Collagen–Alginate Composite Gel Rescues Retinal Photoreceptors from Retinal Degeneration in Rabbits
Abstract
:1. Introduction
2. Results and Discussion
2.1. In Vivo Evaluation of the Effects of CAC ECT Gels on Retinal Structure and Function and Glial Cell Reactivity
2.2. In Vivo Performance of the CAC ECT Gel
2.2.1. Gel Morphology, Encapsulation Power, Mechanical Stability, Material Degradation, and Internal Structure
2.2.2. Encapsulated Cell Viability and GDNF Secretion
2.3. In Vivo Termination of the CAC ECT Gel
2.4. Therapeutic Efficacy of the CAC ECT Gel in Rabbits with Retinal Degeneration
3. Materials and Methods
3.1. Cell Culture of HEK/293/GDNF/Tet-on pro-Casp8
3.2. Preparation of the CAC ECT Gel
3.3. Animal Care and Use
3.4. Intravitreal Gel Injection
3.5. IOP, Binocular Ophthalmoscope (BIO) Examination, and Body Weight Measurement
3.6. ERG Recording
3.7. Retinal Morphological Examination and Analysis
3.8. Immunohistostaining
3.9. In Vivo Gel Safety Study
3.10. In Vivo Gel Performance Study
3.11. In Vivo Gel Termination Study
3.12. In Vivo Therapeutic Potential of the CAC ECT Gel
3.13. Assessment of Cell Viability and Proliferation
3.14. GDNF Quantification
3.15. Scanning Electron Microscope (SEM)
3.16. Terminal Deoxynucleotidyl Transferase (TdT)-Mediated dUTP Nick End Labeling (TUNEL) Assay
3.17. Statistical Analysis
4. Conclusions
5. Limitations of This Study and Prospective Directions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Conflicts of Interest
References
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Unoperated Control | Operated Control | Three-Gel | Six-Gel | |
---|---|---|---|---|
Body weight (kg) | 3.23 ± 0.17 | 3.35 ± 0.12 | 3.27 ± 0.22 | 3.33 ± 0.19 |
IOP (mmHg) | 18.75 ± 0.77 | 17.13 ± 0.74 | 14.27 ± 0.74 | 13.5 ± 0.70 |
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Hu, T.; Zhou, T.; Goit, R.K.; Tam, K.C.; Chan, Y.K.; Lam, W.-C.; Lo, A.C.Y. Bioactive Glial-Derived Neurotrophic Factor from a Safe Injectable Collagen–Alginate Composite Gel Rescues Retinal Photoreceptors from Retinal Degeneration in Rabbits. Mar. Drugs 2024, 22, 394. https://doi.org/10.3390/md22090394
Hu T, Zhou T, Goit RK, Tam KC, Chan YK, Lam W-C, Lo ACY. Bioactive Glial-Derived Neurotrophic Factor from a Safe Injectable Collagen–Alginate Composite Gel Rescues Retinal Photoreceptors from Retinal Degeneration in Rabbits. Marine Drugs. 2024; 22(9):394. https://doi.org/10.3390/md22090394
Chicago/Turabian StyleHu, Tingyu, Ting Zhou, Rajesh Kumar Goit, Ka Cheung Tam, Yau Kei Chan, Wai-Ching Lam, and Amy Cheuk Yin Lo. 2024. "Bioactive Glial-Derived Neurotrophic Factor from a Safe Injectable Collagen–Alginate Composite Gel Rescues Retinal Photoreceptors from Retinal Degeneration in Rabbits" Marine Drugs 22, no. 9: 394. https://doi.org/10.3390/md22090394