Urbanization Impacts the Physicochemical Characteristics and Abundance of Fecal Markers and Bacterial Pathogens in Surface Water
Abstract
:1. Introduction
2. Materials and Methods
2.1. Study Locations
2.2. Field Sampling
2.3. Physicochemical Analyses
2.4. Microbiological Analyses
2.4.1. Culture-Dependent Methods
2.4.2. Culture-Independent Methods (qPCR)
2.5. Statistical Analyses
3. Results
3.1. Variation in Physicochemical Parameters
3.2. Variation in Microbiological Parameters
3.2.1. Culture-Dependent Microbiological Parameters
3.2.2. Detection and Quantification of Fecal Markers
3.2.3. Frequency of Detection and Abundance of Genes of Bacterial Pathogens
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Parameters | Winter 2015 and 2016 Range (Min-Max) | Summer 2015 and 2016 Range (Min-Max) | Control Location (Huangshan) | p Values | |||||
---|---|---|---|---|---|---|---|---|---|
High | Medium | Low | High | Medium | Low | Urbanization | Season | ||
Water temp. °C | 6–11 | 5.1–9.8 | 5.9–10 | 28–34.1 | 26–33.4 | 28.8–34.4 | 24.3–28.2 | 0.001 ** | <0.001 *** |
pH | 7.1–7.9 | 7.51–7.9 | 7.58–7.9 | 7.3–7.7 | 7.39–7.86 | 7.3–8.71 | 7.12–8.16 | 0.006 ** | 0.178 |
Conductivity (µS/cm) | 422–832 | 474–615 | 395–573 | 393–534 | 389–544 | 186–563 | 45.6–146 | 0.013 * | <0.001 *** |
TN (mg/L) | 2.85–16.5 | 2.14–4.48 | 1.57–4.21 | 2.11–4.67 | 1.92–10.58 | 0.96–4.63 | 0.29–1.17 | 0.001 ** | 0.038 * |
TP (mg/L) | 0.13–2.10 | 0.07–0.21 | 0.04–0.26 | 0.23–0.53 | 0.12–1.04 | 0.06–0.21 | 0.02–0.06 | <0.001 | 0.102 |
NO3-N (mg/L) | 1.01–3.42 | 1.13–2.56 | 0.83–2.83 | 0.17–1.01 | 0.05–1.25 | 0.24–1.96 | 0.21–0.92 | 0.816 | <0.001 *** |
NO2-N (mg/L) | 0.01–0.14 | 0.02–0.08 | 0.01–0.12 | 0.05–0.20 | 0.06–0.35 | 0.03–0.27 | 0.00–0.02 | 0.422 | <0.001 *** |
PO4-P (μg/L) | 48–497 | 14.37–121.51 | 11.71–46.45 | 92.78–315 | 30.44–117 | 16.11–88.9 | 3.44–28.93 | <0.001 *** | 0.002 ** |
NH4-N (mg/L) | 1.01–7.84 | 0.18–1.44 | 0.41–1.47 | 0.52–2.40 | 0.23–1.48 | 0.03–2.05 | 0.01–0.10 | <0.001 *** | 0.148 |
TOC (mg/L) | 1.99–42.3 | 3.17–13.23 | 3.55–13.8 | 3.72–20.8 | 3.75–23 | 3.67–15.6 | 1.31–3.35 | 0.936 | 0.745 |
Chlorophyll a (µg/L) | 2.33–21.4 | 1.37–15.41 | 1.47-–6.56 | 3.39–68.86 | 2.7–50.177 | 1.95–54.42 | 0.95–3.17 | 0.329 | <0.001 *** |
Total viable count (× 103 cfu/mL) | 7–57.4 | 0.4–43.9 | 0.6–33.4 | 32–48.7 | 13.4–53.1 | 2–73.7 | 9.5–30.7 | 0.040 * | 0.055 |
Total coliform count (× 103 cfu/mL) | 3.733–10 | 2.067–8.267 | 0.067–0.867 | 3.2–22.1 | 0.2–2.4 | 0–3.9 | 0.098–0.933 | 0.006 ** | 0.696 |
Thermotolerant coliform count (cfu/mL) | 90–120 | 55–85 | 0–23 | 218–480 | 18–253 | 0–233 | 0.5–17 | 0.036 * | 0.032 * |
Sample Type | No. of Samples Tested (n) | No. of Positive Samples a | |||||
---|---|---|---|---|---|---|---|
Enterococcus spp. | Arcobacter butzleri (hsp60) | Shigella (ipaH) | Campylobacter (mapA) | Salmonella spp. | STEC (stx2) | ||
Suzhou | |||||||
Winter 2015 | 9 | 9 (100%) | 8 (89%) | 2 (22%) | 1 (11%) | 0 | 1 (11%) |
Summer 2015 | 9 | 9 (100%) | 9 (100%) | 3 (33%) | 0 | 1 (11%) | 0 |
Winter 2016 | 9 | 9 (100%) | 0 | 2 (22%) | 3 (33%) | 2 (22%) | 7 (78%) |
Summer 2016 | 9 | 9 (100%) | 9 (100%) | 6 (67%) | 0 | 1 (11%) | 6 (67%) |
Huangshan | |||||||
Summer 2016 | 3 | 3 (100%) | 3 (100%) | 1 (33%) | 0 | 0 | 2 (67%) |
Total | 39 | 39 (100%) | 29 (74%) | 14 (36%) | 4 (10%) | 4 (10%) | 16 (41%) |
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Yuan, T.; Vadde, K.K.; Tonkin, J.D.; Wang, J.; Lu, J.; Zhang, Z.; Zhang, Y.; McCarthy, A.J.; Sekar, R. Urbanization Impacts the Physicochemical Characteristics and Abundance of Fecal Markers and Bacterial Pathogens in Surface Water. Int. J. Environ. Res. Public Health 2019, 16, 1739. https://doi.org/10.3390/ijerph16101739
Yuan T, Vadde KK, Tonkin JD, Wang J, Lu J, Zhang Z, Zhang Y, McCarthy AJ, Sekar R. Urbanization Impacts the Physicochemical Characteristics and Abundance of Fecal Markers and Bacterial Pathogens in Surface Water. International Journal of Environmental Research and Public Health. 2019; 16(10):1739. https://doi.org/10.3390/ijerph16101739
Chicago/Turabian StyleYuan, Tianma, Kiran Kumar Vadde, Jonathan D. Tonkin, Jianjun Wang, Jing Lu, Zimeng Zhang, Yixin Zhang, Alan J. McCarthy, and Raju Sekar. 2019. "Urbanization Impacts the Physicochemical Characteristics and Abundance of Fecal Markers and Bacterial Pathogens in Surface Water" International Journal of Environmental Research and Public Health 16, no. 10: 1739. https://doi.org/10.3390/ijerph16101739