Domestic Sewage Treatment Using a One-Stage ANAMMOX Process
Abstract
:1. Introduction
2. Materials and Methods
2.1. Test Device and Method
2.2. Determination Methods
2.3. Microbiological Analytical Methods
3. Results and Discussion
3.1. One-stage ANAMMOX Reactor Start-up
3.2. Nitrogen Load Acclimation Stage
3.3. One-stage ANAMMOX Reactor for Treating Real Domestic Sewage
3.4. Microbial Diversity Analysis
4. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
- National Bureau of Statistics of China. National Data. Annual Data. Daily Treatment Capacity of Urban Sewage. 2020. Available online: http:data.stats.gov.cn (accessed on 25 March 2020).
- Ma, X.; Liu, X.; Xiang, B.; Zhang, W. Effect of Hydraulic Retention Time on Carbon Sequestration during the Two-Stage Anammox Process. Processes 2019, 7, 717. [Google Scholar] [CrossRef] [Green Version]
- Gong, L.; Huo, M.; Yang, Q.; Li, J.; Ma, B.; Zhu, R.; Wang, S.; Peng, Y. Performance of heterotrophic partial denitrification under feast-famine condition of electron donor: A case study using acetate as external carbon source. Bioresour. Technol. 2013, 133, 263–269. [Google Scholar] [CrossRef]
- Wen, R.; Jin, Y.; Zhang, W. Application of the Anammox in China—A Review. Int. J. Environ. Res. Public Health. 2020, 17, 1090. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Van de Graaf, A.A.; Mulder, A.; de Bruijin, P.; Jetten, M.S.; Robertson, L.A.; Kuenen, J.G. Anaerobic oxidation of ammonium is a biologically mediated process. Appl. Environ. Microbiol. 1995, 61, 1246–1251. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Strous, M.; Kuenen, G.J. Key Physiology of Anaerobic Ammonium Oxidation. Appl. Environ. Microbiol. 1999, 7, 3248–3250. [Google Scholar] [CrossRef] [Green Version]
- Kartal, B.; Maalcke, W.J.; De Almeida, N.M.; Cirpus, I.; Gloerich, J.; Geerts, W.; Op Den Camp, H.J.M.; Harhangi, H.R.; Janssen-Megens, E.M.; Francoijs, K.J.; et al. Molecular mechanism of anaerobic ammonium oxidation. Nature 2011, 7371, 127–130. [Google Scholar] [CrossRef]
- Kuenen, J.G. Timeline: Anammox bacteria: From discovery to application. Nat. Rev. Microbiol. 2008, 6, 320–326. [Google Scholar] [CrossRef]
- Van Hulle, S.W.H.; Vandeweyer, H.J.P.; Meesschaert, B.D.; Vanrolleghem, P.A.; Dejans, P.; Dumoulin, A. Engineering aspects and practical application of autotrophic nitrogen removal from nitrogen rich streams. Chem. Eng. J. 2010, 162, 1–20. [Google Scholar] [CrossRef]
- Kartal, B.; Kuenen, J.G.; van Loosdrecht, M.C.M. Sewage Treatment with Anammox. Science 2010, 328, 702–703. [Google Scholar] [CrossRef]
- Lackner, S.; Gilbert, E.M.; Vlaeminck, S.E.; Joss, A.; Horn, H.; van Loosdrecht, M.C.M. Full-scale partial nitritation/anammox experiences—An application survey. Water Res. 2014, 55, 292–303. [Google Scholar] [CrossRef]
- Jetten, M.S.; Wagner, M.; Fuerst, J.; van Loosdrecht, M.; Kuenen, G.; Strous, M. Microbiology and application of the anaerobic ammonium oxidation (‘anammox’) process. Curr. Opin. Biotechnol. 2001, 12, 283–288. [Google Scholar] [CrossRef]
- Chen, Z.; Meng, Y.; Sheng, B.; Zhou, Z.; Jin, C.; Meng, F. Linking Exoproteome Function and Structure to Anammox Biofilm Development. Environ. Sci. Technol. 2019, 53, 1490–1500. [Google Scholar] [CrossRef] [PubMed]
- Liu, X.; Wang, H.; Li, H.; Jin, Y.; Zhang, W. Carbon sequestration pathway of inorganic carbon in partial nitrification sludge. Bioresour. Technol. 2019, 293, 122101. [Google Scholar] [CrossRef] [PubMed]
- Azari, M.; Walter, U.; Rekers, V.; Gu, J.D.; Denecke, M. More than a decade of experience of landfill leachate treatment with a full-scale anammox plant combining activated sludge and activated carbon biofilm. Chemosphere 2017, 174, 117–126. [Google Scholar] [CrossRef] [PubMed]
- Hu, Z.; Lotti, T.; van Loosdrecht, M.; Kartal, B. Nitrogen removal with the anaerobic ammonium oxidation process. Biotechnol. Lett. 2013, 35, 1145–1154. [Google Scholar] [CrossRef]
- Wang, X.; Yang, R.; Zhang, Z.; Wu, J.; Chen, S. Mass balance and bacterial characteristics in an in-situ fullscale swine wastewater treatment system occurring anammox process. Bioresour. Technol. 2019, 292, 122005. [Google Scholar] [CrossRef]
- Ali, M.A.M.; Okabe, S.O.S. Anammox-based technologies for nitrogen removal: Advances in process start-up and remaining issues. Chemosphere 2015, 141, 144–153. [Google Scholar] [CrossRef]
- Li, X.; Zhang, J.; Zhang, X.; Li, J.; Liu, F.; Chen, Y. Start-up and nitrogen removal performance of CANON and SNAD processes in a pilot-scale oxidation ditch reactor. Process Biochem. 2019, 84, 134–142. [Google Scholar] [CrossRef]
- Kazuichi, I.; Tatsuo, S.; Satoshi, T. High Nitrogen Removal Performance at Moderately Low Temperature Utilizing Anaerobic Ammonium Oxidation Reactions. J. Biosci. Bioeng. 2007, 103, 486–490. [Google Scholar]
- Strous, M.; Heijnen, J.J.; Kuenen, J.G.; Jetten, M.S.M. The sequencing batch reactor as a powerful tool for the study of slowly growing anaerobic ammonium-oxidizing microorganisms. Appl. Microbiol. Biotechnol. 1998, 50, 589–596. [Google Scholar] [CrossRef]
- Khramenkov, S.V.; Kozlov, M.N.; Kevbrina, M.V.; Dorofeev, A.G.; Kazakova, E.A.; Grachev, V.A.; Kuznetsov, B.B.; Polyakov, D.Y.; Nikolaev, Y.A. A novel bacterium carrying OTUs anaerobic ammonium oxidation in a reactor for biological treatment of the filtrate of wastewater fermented sludge. Microbiology 2013, 82, 628–636. [Google Scholar] [CrossRef]
- Jin, Y.; Wang, D.; Zhang, W. Treatment of high-strength ammonia containing wastewater using partial nitritation system with biological selector. Glob. NEST J. 2015, 17, 738–743. [Google Scholar]
- Jin, Y.; Wang, D.; Zhang, W. Use of bamboo charcoal reduced the cultivated anammox seed sludge dosage during the start-up period. Desalin. Water Treat. 2016, 57, 20248–20253. [Google Scholar] [CrossRef]
- Zhang, W.; Liu, X.; Wang, D.; Jin, Y. Effects of bamboo charcoal on fouling and microbial diversity in a flatsheet ceramic membrane bioreactor. Bioresour. Technol. 2017, 243, 1020–1026. [Google Scholar] [CrossRef]
- Zhang, W.; Wang, D.; Jin, Y. Use of a ceramic membrane bioreactor (CMBR) to treat wastewater at Guilin University of Technology. Water Pract. 2017, 12, 453–462. [Google Scholar] [CrossRef]
- Liu, X.; Jin, Y.; Zhang, W. Effect of nitrite concentration on the growth and microbial diversity of anaerobic ammonia oxidation (anammox) sludge. Desalin. Water Treat. 2020, 179, 54–62. [Google Scholar] [CrossRef]
- Yin, Z.Y.Z.; Dos Santos, C.D.D.S.; Vilaplana, J.V.J.G.; Sobotka, D.S.D.; Czerwionka, K.C.K.; Damianovic, M.R.Z.D.; Xie, L.X.L.; Morales, F.F.M.F.; Makinia, J.M.J. Importance of the combined effects of dissolved oxygen and pH on optimization of nitrogen removal in anammox-enriched granular sludge. Process Biochem. 2016, 51, 1274–1282. [Google Scholar] [CrossRef]
- American Public Health Association (APHA). Standard Method for the Examination of Water and Wastewater, 19th ed.; American Public Health Association: Washington, DC, USA, 1995. [Google Scholar]
- Jin, Y.; Xie, Q.; Zhang, W. High-strength ethylene glycol wastewater treatment in anaerobic polyvinyl alcohor gel beads based biofilm reactor. Glob. NEST J. 2016, 18, 47–55. [Google Scholar]
- Hui, C.; Hai, Y.; Qian, Q.; Man, L.; Ren, C. Successful start-up of the anammox process: Influence of the seeding strategy on performance and granule properties. Bioresour. Technol. 2016, 211, 594–602. [Google Scholar]
- Kato, S.; Haruta, S.; Cui, Z.J.; Ishii, M.; Igarashi, Y. Stable Coexistence of Five Bacterial Strains as a Cellulose-Degrading Community. Appl. Environ. Microbiol. 2005, 71, 7099–7106. [Google Scholar] [CrossRef] [Green Version]
- Goel, R.; Mino, T.; Satoh, H.; Matsuo, T. Effect of electron acceptor conditions on hydrolytic enzyme synthesis in bacterial cultures. Water Res. 1997, 31, 2597–2603. [Google Scholar] [CrossRef]
- Grady, C.P.L., Jr.; Daigger, G.T.; Love, N.G. Biological Wastewater Treatment; IWA Publishing: London, UK, 2011. [Google Scholar]
- Park, H.D.; Noguera, D.R. Evaluating the effect of dissolved oxygen on ammonia-oxidizing bacterial communities in activated sludge. Water Res. 2004, 38, 3275–3286. [Google Scholar] [CrossRef] [PubMed]
- Kwon, S.; Kim, T.S.; Yu, G.H.; Jung, J.H.; Park, H.D. Bacterial Community Composition and Diversity of a Full-Scale Integrated Fixed-Film Activated Sludge System as Investigated by Pyrosequencing. J. Microbiol. Biotechnol. 2010, 20, 1717–1723. [Google Scholar]
- Lang, X.; Li, Q.; Ji, M.; Yan, G.; Guo, S. Isolation and niche characteristics in simultaneous nitrification and denitrification application of an aerobic denitrifier, Acinetobacter sp. YS2. Bioresour. Technol. 2020, 302, 122799. [Google Scholar] [CrossRef] [PubMed]
- Lambert, S.E.; Randall, D.G. Manufacturing bio-bricks using microbial induced calcium carbonate precipitation and human urine. Water Res. 2019, 160, 158–166. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Strous, M.; Fuerst, J.A.; Kramer, E.H.; Logemann, S.; Muyzer, G.; van de Pas-Schoonen, K.T.; Webb, R.; Kuenen, J.G.; Jetten, M.S. Missing lithotroph identified as new planctomycete. Nature 1999, 400, 446–449. [Google Scholar] [CrossRef]
- Kartal, B.; van Niftrik, L.; Rattray, J.; van de Vossenberg Jack, L.C.M.; Schmid Markus, C.; Sinninghe Damsté, J.; Jetten Mike, S.M. Strous Marc. Candidatus ‘Brocadia fulgida’: An autofluorescent anaerobic ammonium oxidizing bacterium. FEMS Microbiol. Ecol. 2008, 63, 46–55. [Google Scholar] [CrossRef]
- Liu, S.; Yang, F.; Gong, Z.; Meng, F.; Chen, H.; Xue, Y.; Kenji, F. Application of anaerobic ammonium-oxidizing consortium to achieve completely autotrophic ammonium and sulfate removal. Bioresour. Technol. 2008, 99, 6817–6825. [Google Scholar] [CrossRef]
- Van der Star Wouter, R.L.; Abma Wiebe, R.; Blommers, D.; Mulder, J.-W.; Tokutomi, T.; Strous, M.; Picioreanu, C.; van Loosdrecht Mark, C.M. Startup of reactors for anoxic ammonium oxidation Experiences from the first fullscale anammox reactor in Rotterdam. Water Res. 2007, 41, 4149–4163. [Google Scholar] [CrossRef]
Parameter | Unit | Startup Stage | High Nitrogen Load Acclimation Stage | Domestic Sewage |
---|---|---|---|---|
Chemical oxygen demand (COD) | mg/L | -- | -- | 140–160 |
NH4+-N | mg/L | 250–200 | 200 ± 5 | 50–70 |
Total nitrogen (TN) | mg/L | 250–200 | 200 ± 5 | 70–80 |
Total phosphorus (TP) | mg/L | -- | -- | 3–4 |
KH2PO4 | mg/L | 25 | 25 | -- |
NaHCO3 | mg/L | 1000 | 1000 | -- |
CaCl2·2H2O | mg/L | 113 | 113 | -- |
MgSO4·7H2O | mg/L | 100 | 100 | -- |
pH | -- | 7.5–7.6 | 7.5–7.6 | 6.7–8.2 |
Temperature | °C | 30 ± 1 | 30 ± 1 | 23.2–26.7 |
Stage | Duration (d) | HRT (h) | Temperature (°C) | pH | DO (mg /L) | Cycle Ratio |
---|---|---|---|---|---|---|
Start-up | 1–37 | 24 | 30 ± 1 | 7.5–7.6 | < 0.6 | 4:1 |
High nitrogen load acclimation | 38–108 | 15-4 | 30 ± 1 | 7.5–7.6 | < 0.6 | 4:1 |
Treatment of domestic sewage | 109–129 | 4-6 | 30 ± 1 | 7.5–7.6 | < 0.6 | 4:1 |
Phase | Duration (d) | NLR (kg-N/m3/d) | HRT (h) | Temperature (°C) | pH | DO (mg /L) | Cycle Ratio |
---|---|---|---|---|---|---|---|
I | 1–15 | 0.32 | 15 | 30 ± 1 | 7.5–7.6 | < 0.6 | 4:1 |
II | 16–27 | 0.40 | 12 | 30 ± 1 | 7.5–7.6 | < 0.6 | 4:1 |
III | 28–34 | 0.60 | 8 | 30 ± 1 | 7.5–7.6 | < 0.6 | 4:1 |
IV | 35–63 | 0.80 | 6 | 30 ± 1 | 7.5–7.6 | < 0.6 | 4:1 |
V | 64–71 | 1.20 | 4 | 30 ± 1 | 7.5–7.6 | < 0.6 | 4:1 |
Sample | Quality Sequence | OTUs | Chaol | Shannon | Coverage |
---|---|---|---|---|---|
T1 | 34878 | 381 | 533.24 | 1.84 | 0.9960 |
T2 | 45936 | 440 | 595.18 | 2.15 | 0.9965 |
T3 | 44230 | 430 | 564.57 | 1.78 | 0.9965 |
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Wei, Y.; Jin, Y.; Zhang, W. Domestic Sewage Treatment Using a One-Stage ANAMMOX Process. Int. J. Environ. Res. Public Health 2020, 17, 3284. https://doi.org/10.3390/ijerph17093284
Wei Y, Jin Y, Zhang W. Domestic Sewage Treatment Using a One-Stage ANAMMOX Process. International Journal of Environmental Research and Public Health. 2020; 17(9):3284. https://doi.org/10.3390/ijerph17093284
Chicago/Turabian StyleWei, Yuan, Yue Jin, and Wenjie Zhang. 2020. "Domestic Sewage Treatment Using a One-Stage ANAMMOX Process" International Journal of Environmental Research and Public Health 17, no. 9: 3284. https://doi.org/10.3390/ijerph17093284