In Vitro and In Vivo Evaluation of Nanostructured Biphasic Calcium Phosphate in Granules and Putty Configurations
Abstract
:1. Introduction
2. Materials and Methods
2.1. Biomaterial Composition
2.2. Physico-Chemical Characterization of Biomaterials
2.3. In Vitro Assay
2.4. In Vivo Analysis
2.4.1. Ethical Considerations
2.4.2. Animal Characterization and Location
2.4.3. Anesthesia and Surgery Procedures
2.4.4. Image Acquisition by Micro-CT and 3D Segmentation
2.4.5. Segmentation Protocol
2.4.6. Histological Processing
2.4.7. Histological and Histomorphometric Evaluation
2.4.8. Statistical Analysis
3. Results
3.1. Biomaterial-Related Characterization
3.2. Cytocompatibility
3.3. Descriptive Histology
3.3.1. One Month
3.3.2. Three Months
3.3.3. Six Months
3.4. Histomorphometric Results
3.5. Three-Dimensional Evaluation by Micro-CT
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Nascimento, J.R.B.; Sartoretto, S.C.; Alves, A.T.N.N.; Mourão, C.F.A.B.; Martinez-Zelaya, V.R.; Uzeda, M.J.; Granjeiro, J.M.; Montemezzi, P.; Calasans-Maia, M.D.; Calasans-Maia, J.A. In Vitro and In Vivo Evaluation of Nanostructured Biphasic Calcium Phosphate in Granules and Putty Configurations. Int. J. Environ. Res. Public Health 2021, 18, 533. https://doi.org/10.3390/ijerph18020533
Nascimento JRB, Sartoretto SC, Alves ATNN, Mourão CFAB, Martinez-Zelaya VR, Uzeda MJ, Granjeiro JM, Montemezzi P, Calasans-Maia MD, Calasans-Maia JA. In Vitro and In Vivo Evaluation of Nanostructured Biphasic Calcium Phosphate in Granules and Putty Configurations. International Journal of Environmental Research and Public Health. 2021; 18(2):533. https://doi.org/10.3390/ijerph18020533
Chicago/Turabian StyleNascimento, Jhonathan R. B., Suelen C. Sartoretto, Adriana T. N. N. Alves, Carlos F. A. B. Mourão, Victor R. Martinez-Zelaya, Marcelo J. Uzeda, José M. Granjeiro, Pietro Montemezzi, Monica D. Calasans-Maia, and José A. Calasans-Maia. 2021. "In Vitro and In Vivo Evaluation of Nanostructured Biphasic Calcium Phosphate in Granules and Putty Configurations" International Journal of Environmental Research and Public Health 18, no. 2: 533. https://doi.org/10.3390/ijerph18020533