MALDI-TOF Mass Spectrometry Analysis and Human Post-Mortem Microbial Community: A Pilot Study
Abstract
:1. Introduction
2. Materials and Methods
2.1. Autopsy and Sampling
2.2. Microbiological Analysis
2.3. Bacterial Identification
3. Results
4. Discussion
5. Strengths and Limitations
6. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Case # | Age/Sex | Death Scene | PMI (h) | Season | Stage of Decay | Cause of Death | Manner of Death |
---|---|---|---|---|---|---|---|
1 | 53 y/M | Indoor (Home) | 24–36 | Winter | Fresh | Hanging | Suicide |
2 | 61 y/M | Indoor (Home) | 96–120 | Winter | Fresh with early signs of decomposition | Blunt injuries to the head | Homicide |
3 | 38 y/M | Outdoor | 36–48 | Winter | Fresh | Fatal fall from great height | Suicide |
4 | 57 y/M | Indoor (Hospital) | 72–96 | Spring | Fresh with early signs of decomposition | Respiratory failure due to pneumonia | Natural |
5 | 40 y/M | Indoor (Hospital) | 288–312 | Winter | Fresh/Bloated | Multi Organ Failure | Natural |
6 | 45 y/F | Indoor (Hospital) | 216–240 | Winter | Fresh/Bloated | Septic shock | Natural |
7 | 45 y/M | Indoor (Hospital) | 72–96 | Spring | Fresh | Gunshot injuries to the head | Homicide |
8 | 20 y/M | Indoor (Hospital) | 72–96 | Spring | Fresh | Blunt injuries to the head | Homicide |
9 | 80 y/M | Indoor (Hospital) | 36–48 | Spring | Fresh | Blunt Trauma | Road Accident |
10 | 6 m/M | Indoor (Home) | 24–36 | Spring | Fresh | Respiratory failure due to pneumonia | Natural |
11 | 51 y/M | Indoor (Prison) | 96–120 | Spring | Fresh with early signs of decomposition | Subarachnoid hemorrhage | Natural |
12 | 82 y/M | Indoor (Hospital) | 336–360 | Summer | Fresh/Bloated | Fatal fall from great height | Suicide |
13 | 4 m/M | Indoor (Home) | 24–36 | Summer | Fresh | Respiratory failure due to pneumonia | Natural |
14 | 41 y/M | Indoor (Hospital) | 168–192 | Summer | Fresh/Bloated | Liver failure in cirrhosis | Natural |
15 | 20 y/M | Indoor (Hospital) | 96–120 | Winter | Fresh with early signs of decomposition | Blunt Trauma | Road accident |
16 | 53 y/M | Outdoor | 240–264 | Spring | Bloated | Myocardial infarction due to coronary artery disease | Natural |
17 | 22 y/M | Indoor (Hospital) | 36–48 | Spring | Fresh | Blunt Trauma | Road accident |
18 | 70 y/M | Outdoor | 36–48 | Summer | Fresh | Arrhythmogenic cardiomyopathy | Natural |
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Campobasso, C.P.; Mastroianni, G.; Feola, A.; Mascolo, P.; Carfora, A.; Liguori, B.; Zangani, P.; Dell’Annunziata, F.; Folliero, V.; Petrillo, A.; et al. MALDI-TOF Mass Spectrometry Analysis and Human Post-Mortem Microbial Community: A Pilot Study. Int. J. Environ. Res. Public Health 2022, 19, 4354. https://doi.org/10.3390/ijerph19074354
Campobasso CP, Mastroianni G, Feola A, Mascolo P, Carfora A, Liguori B, Zangani P, Dell’Annunziata F, Folliero V, Petrillo A, et al. MALDI-TOF Mass Spectrometry Analysis and Human Post-Mortem Microbial Community: A Pilot Study. International Journal of Environmental Research and Public Health. 2022; 19(7):4354. https://doi.org/10.3390/ijerph19074354
Chicago/Turabian StyleCampobasso, Carlo Pietro, Gennaro Mastroianni, Alessandro Feola, Pasquale Mascolo, Anna Carfora, Bruno Liguori, Pierluca Zangani, Federica Dell’Annunziata, Veronica Folliero, Arianna Petrillo, and et al. 2022. "MALDI-TOF Mass Spectrometry Analysis and Human Post-Mortem Microbial Community: A Pilot Study" International Journal of Environmental Research and Public Health 19, no. 7: 4354. https://doi.org/10.3390/ijerph19074354