Chromium Speciation by HPLC-DAD/ICP-MS: Simultaneous Hyphenation of Analytical Techniques for Studies of Biomolecules
Abstract
:1. Introduction
2. Materials and Methods
2.1. Reagents and Instruments
2.2. Chemical Speciation of Chromium
2.3. Extraction of Chromium Chemical Species
2.4. Figures of Merit and Chromatographic Parameters of the Method
3. Results and Discussion
3.1. Development of “T Piece” and Evaluation of the Cr Chemical Speciation
3.2. Evaluation of the Hyphenation Injection of Real Samples in the HPLC-DAD/ICP-MS
3.3. Evaluation of Figures of Merit and Chromatographic Parameters
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Column | Hamilton PRP-X100 (5 µm, 150 mm × 4.6 mm) |
---|---|
Mobile phase | NH4NO3 225 mM at pH = 7 |
Mobile phase flow | 0.45–0.5 mL min−1 |
Elution type | Isocratic |
Race time | 8 min |
Rinsing | 3 wash cycles |
Injection volume | 30 µL |
Quantification | Peak area |
Radio frequency power | 1550 W |
Gas flow (Ar) | 15 L min−1 |
Nebulizing gas flow | 0.9 L min−1 |
Interface | Nickel cones |
Sampling cone | 0.9 mm |
Extractor cone (skimmer) | 0.45 mm |
Nebulizer | Micro MistTM |
Monitored Isotope | 53Cr |
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Procópio, V.A.; Pereira, R.M.; Lange, C.N.; Freire, B.M.; Batista, B.L. Chromium Speciation by HPLC-DAD/ICP-MS: Simultaneous Hyphenation of Analytical Techniques for Studies of Biomolecules. Int. J. Environ. Res. Public Health 2023, 20, 4912. https://doi.org/10.3390/ijerph20064912
Procópio VA, Pereira RM, Lange CN, Freire BM, Batista BL. Chromium Speciation by HPLC-DAD/ICP-MS: Simultaneous Hyphenation of Analytical Techniques for Studies of Biomolecules. International Journal of Environmental Research and Public Health. 2023; 20(6):4912. https://doi.org/10.3390/ijerph20064912
Chicago/Turabian StyleProcópio, Vitória Aparecida, Rodrigo Mendes Pereira, Camila Neves Lange, Bruna Moreira Freire, and Bruno Lemos Batista. 2023. "Chromium Speciation by HPLC-DAD/ICP-MS: Simultaneous Hyphenation of Analytical Techniques for Studies of Biomolecules" International Journal of Environmental Research and Public Health 20, no. 6: 4912. https://doi.org/10.3390/ijerph20064912