Whey Derivatives and Galactooligosaccharides Stimulate the Wound Healing and the Function of Human Keratinocytes through the NF-kB and FOXO-1 Signaling Pathways
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cells and Reagents
2.2. Preparation of Whey Derivatives
2.3. Cell Treatments
2.4. Cell Proliferation Assay
2.5. Cell Viability Assays
2.6. Wound Healing Assay
2.7. Real-Time Polymerase Chain Reaction (qRT-PCR)
2.8. Western Blot Analysis
2.9. Statistical Analysis
3. Results
3.1. GOS Induce the Production of IL-8 through NF-kB Signaling
3.2. Whey Derivatives and GOS Increase the Motility of HaCaT Cells
3.3. GOS Enhance Mitochondrial Activity in Association with Mitochondrial Export of FOXO-1
3.4. Whey Derivatives and GOS Increase the Differentiation of HaCaT Cells
4. Discussion
5. Conclusions
6. Patents
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Bergandi, L.; Flutto, T.; Valentini, S.; Thedy, L.; Pramotton, R.; Zenato, S.; Silvagno, F. Whey Derivatives and Galactooligosaccharides Stimulate the Wound Healing and the Function of Human Keratinocytes through the NF-kB and FOXO-1 Signaling Pathways. Nutrients 2022, 14, 2888. https://doi.org/10.3390/nu14142888
Bergandi L, Flutto T, Valentini S, Thedy L, Pramotton R, Zenato S, Silvagno F. Whey Derivatives and Galactooligosaccharides Stimulate the Wound Healing and the Function of Human Keratinocytes through the NF-kB and FOXO-1 Signaling Pathways. Nutrients. 2022; 14(14):2888. https://doi.org/10.3390/nu14142888
Chicago/Turabian StyleBergandi, Loredana, Tania Flutto, Sabina Valentini, Laura Thedy, Rita Pramotton, Simona Zenato, and Francesca Silvagno. 2022. "Whey Derivatives and Galactooligosaccharides Stimulate the Wound Healing and the Function of Human Keratinocytes through the NF-kB and FOXO-1 Signaling Pathways" Nutrients 14, no. 14: 2888. https://doi.org/10.3390/nu14142888