Effects of Additional Xylanase on Saccharification and Ethanol Fermentation of Ammonia-Pretreated Corn Stover and Rice Straw
Abstract
:1. Introduction
2. Materials and Methods
2.1. Feedstock
2.2. Enzymes
2.3. Pretreatment
2.4. Enzymatic Digestibility
2.5. Simultaneous Saccharification and Fermentation (SSF) and the Simultaneous Saccharification and Co-Fermentation (SSCF)
2.6. Compositional Analysis
3. Results and Discussion
3.1. Enzymatic Saccharification of LMAA-Treated Corn Stover and Rice Straw
3.2. Effects of LMAA Pretreatment on Chemical Compositions of Corn Stover and Rice Straw
3.3. Effect of Additional Xylanase on the Enzymatic Hydrolysis of LMAA-Treated Biomass
3.4. SSF and SSCF of LMAA-Treated Biomass
3.5. Effect of Different Xylanase Dosages on Ethanol Productions in SSF and SSCF Processes Using LMAA-Treated Corn Stover
3.6. High-Solid Fermentation Using Fed-Batch SSF and SSCF
3.7. Selection of Inoculum Size for Ethanol Production in SSCF
4. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Component | Corn Stover | Rice Straw | |||
---|---|---|---|---|---|
Untreated | LMAA-Treated | Untreated | LMAA-Treated | ||
Glucan | [%] | 31.6 ± 0.3 | 31.5 ± 0.4 | 34.7 ± 0.5 | 34.8 ± 0.3 |
XMG a | [%] | 22.6 ± 0.9 | 22.5 ± 0.8 | 20.2 ± 0.6 | 20.1 ± 0.3 |
AIL b | [%] | 16.2 ± 0.8 | 15.5 ± 0.6 | 15.7 ± 0.8 | 15.1 ± 0.2 |
ASL c | [%] | 1.8 ± 0.2 | 2.5 ± 0.3 | 1.6 ± 0.3 | 2.2 ± 0.1 |
Ash | [%] | 0.7 ± 0.2 | 0.8 ± 0.2 | 8.4 ± 0.3 | 9.2 ± 0.2 |
Source of Variation | SS | DF | MS | F | p-Value | Fcrit |
---|---|---|---|---|---|---|
Between Groups | 12.35 | 5 | 2.47 | 5.34 | 0.03 | 4.39 |
Within Groups | 2.78 | 6 | 0.46 | |||
Total | 15.12 | 11 |
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Park, S.H.; Pham, T.T.H.; Kim, T.H. Effects of Additional Xylanase on Saccharification and Ethanol Fermentation of Ammonia-Pretreated Corn Stover and Rice Straw. Energies 2020, 13, 4574. https://doi.org/10.3390/en13174574
Park SH, Pham TTH, Kim TH. Effects of Additional Xylanase on Saccharification and Ethanol Fermentation of Ammonia-Pretreated Corn Stover and Rice Straw. Energies. 2020; 13(17):4574. https://doi.org/10.3390/en13174574
Chicago/Turabian StylePark, Seung Hyeon, Thi Thu Huong Pham, and Tae Hyun Kim. 2020. "Effects of Additional Xylanase on Saccharification and Ethanol Fermentation of Ammonia-Pretreated Corn Stover and Rice Straw" Energies 13, no. 17: 4574. https://doi.org/10.3390/en13174574