Modeling of the Human Bone Environment: Mechanical Stimuli Guide Mesenchymal Stem Cell–Extracellular Matrix Interactions
Abstract
:1. Introduction
2. Materials and Methods
2.1. Preparation of Decellularized Bone Scaffolds
2.2. Elastic Modulus Measurements
2.3. Pore Size Measurements
2.4. Dynamic Bioreactor
2.5. Computational Fluid Dynamic
2.6. Mesenchymal Stem/Stromal Cells Isolation and Loading Protocol
2.7. Viability Assays
2.8. Immunofluorescence Analysis
2.9. Scanning Electron Microscopy and Energy Dispersive X-ray Spectroscopy
2.10. Gene Transcription Analysis
2.11. Statistical Analysis
3. Results
3.1. Scaffold Structure Characterization
3.2. Computational Modeling
3.3. In Vitro Studies
3.3.1. hMSC—Scaffold Integration in the Static and Dynamic Culture
3.3.2. hMSC Early Response to Mechanical Stimuli
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Pereira, A.R.; Lipphaus, A.; Ergin, M.; Salehi, S.; Gehweiler, D.; Rudert, M.; Hansmann, J.; Herrmann, M. Modeling of the Human Bone Environment: Mechanical Stimuli Guide Mesenchymal Stem Cell–Extracellular Matrix Interactions. Materials 2021, 14, 4431. https://doi.org/10.3390/ma14164431
Pereira AR, Lipphaus A, Ergin M, Salehi S, Gehweiler D, Rudert M, Hansmann J, Herrmann M. Modeling of the Human Bone Environment: Mechanical Stimuli Guide Mesenchymal Stem Cell–Extracellular Matrix Interactions. Materials. 2021; 14(16):4431. https://doi.org/10.3390/ma14164431
Chicago/Turabian StylePereira, Ana Rita, Andreas Lipphaus, Mert Ergin, Sahar Salehi, Dominic Gehweiler, Maximilian Rudert, Jan Hansmann, and Marietta Herrmann. 2021. "Modeling of the Human Bone Environment: Mechanical Stimuli Guide Mesenchymal Stem Cell–Extracellular Matrix Interactions" Materials 14, no. 16: 4431. https://doi.org/10.3390/ma14164431