Application of Liposome Encapsulating Lactobacillus curvatus Extract in Cosmetic Emulsion Lotion
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Extraction of L. curvatus
2.3. Fabrication of L. curvatus Extract Encapsulated Liposome and Characterization
2.3.1. Liposome Preparation
2.3.2. Zeta-Potential and Particle Size
2.3.3. Encapsulation Efficiency of Liposomes
2.3.4. Permeability of Liposomes
2.4. Preparation of Cosmetic O/W Emulsion
2.5. Characterization of Microscopic Properties and Shear Stress of Cosmetic O/W Lotion Emulsion
2.5.1. Characterization of Microscopic Properties
Zeta–Potential Measurement
Droplet Size Measurement
Optical Microscopy
2.5.2. Characterization of Shear Stress of Cosmetic O/W Emulsion
2.6. Functional Test of Cosmetic O/W Emulsion
2.6.1. Radical Scavenging Activity
DPPH
ABTS
2.6.2. In Vitro Tyrosinase Inhibition Test
2.6.3. Challenge Test
2.7. Statistical Analysis
3. Results and Discussion
3.1. Characterization of L. curvatus Extract Encapsulated Liposome
3.2. Characterization of Liposome-Containing O/W Emulsion
3.2.1. Characterization of Microscopic Properties
3.2.2. Characterization of Shear Stress
3.3. Functional Tests of Liposome-Containing O/W Emulsion
3.3.1. Radical Scavenging Activity
3.3.2. In Vitro Tyrosinase Inhibition
3.3.3. Challenge Test
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Abbreviations
O/W | Oil-in-water |
E | Extract of Lactobacillus curvatus |
BL | Blank liposome |
EL | Liposome-encapsulated extract of L. curvatus |
B-L | Blank lotion |
BL-L | Lotion with blank liposome |
E-L | Lotion with L. curvatus extract |
EL-L | Lotion with L. curvatus extract encapsulated with liposome |
D-E | Extract of L. curvatus diluted into 62% |
D-EL | Liposome-encapsulated extract of L. curvatus diluted into 62% |
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Treatments | Composition (%) | ||||||||
---|---|---|---|---|---|---|---|---|---|
Water Phase | Oil Phase | ||||||||
Water | Liposome | Extract | Liposome Coating Extract | Glycerin | Tween 80 | Carrageenan | MCT Oil | Lecithin | |
B-L | 62.0 | - | - | - | 7.0 | 0.7 | 0.3 | 28.5 | 1.5 |
BL-L | - | 62.0 | - | - | 7.0 | 0.7 | 0.3 | 28.5 | 1.5 |
E-L | - | - | 62.0 | - | 7.0 | 0.7 | 0.3 | 28.5 | 1.5 |
EL-L | - | - | - | 62.0 | 7.0 | 0.7 | 0.3 | 28.5 | 1.5 |
Treatments | Particle Size (nm) | PdI | [-] ζ-Potential (mV) | Encapsulation Efficiency (%) | Permeability (%) |
---|---|---|---|---|---|
E | 240.92 ± 1.95 c | 0.23 ± 0.01 a | 10.40 ± 0.16 c | - | - |
BL | 125.60 ± 0.65 b | 0.26 ± 0.00 c | 35.43 ± 0.26 b | 98.09 ± 1.13 | 60.29 ± 0.92 |
EL | 174.80 ± 3.37 a | 0.34 ± 0.02 b | 52.53 ± 0.52 a | 52.46 ± 0.63 | 96.71 ± 1.07 |
Treatments | Droplet Size d [4,3] (µm) | [-] ζ-Potential (mV) |
---|---|---|
B-L | 3.54 ± 0.02 c | 58.57 ± 0.33 ab |
BL-L | 4.37 ± 0.18 a | 59.33 ± 0.59 a |
E-L | 3.69 ± 0.03 b | 58.47 ± 0.24 ab |
EL-L | 4.23 ± 0.19 a | 58.23 ± 0.21 b |
Treatments | DPPH (%) | ABTS (%) |
---|---|---|
BHA | 81.76 ± 1.44 a | 97.16 ± 0.45 |
D-E | 5.57 ± 0.74 c | 46.77 ± 1.61 |
E | 6.29 ± 0.68 c | 52.97 ± 1.79 |
D-EL | 59.40 ± 3.58 b | 48.00 ± 3.25 |
EL | 58.36 ± 6.42 a | 73.42 ± 1.28 |
B-L | 65.20 ± 8.41 b | 9.30 ± 6.02 |
E-L | 54.32 ± 10.73 b | 35.14 ± 15.76 |
EL-L | 60.63 ± 7.50 b | 68.88 ± 8.84 |
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Kim, H.-T.; Lee, J.; Jo, Y.-J.; Choi, M.-J. Application of Liposome Encapsulating Lactobacillus curvatus Extract in Cosmetic Emulsion Lotion. Materials 2021, 14, 7571. https://doi.org/10.3390/ma14247571
Kim H-T, Lee J, Jo Y-J, Choi M-J. Application of Liposome Encapsulating Lactobacillus curvatus Extract in Cosmetic Emulsion Lotion. Materials. 2021; 14(24):7571. https://doi.org/10.3390/ma14247571
Chicago/Turabian StyleKim, Hyo-Tae, Jiseon Lee, Yeon-Ji Jo, and Mi-Jung Choi. 2021. "Application of Liposome Encapsulating Lactobacillus curvatus Extract in Cosmetic Emulsion Lotion" Materials 14, no. 24: 7571. https://doi.org/10.3390/ma14247571