Curdlan-Based Hydrogels for Potential Application as Dressings for Promotion of Skin Wound Healing—Preliminary In Vitro Studies
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Preparation of Curdlan-Based Biomaterials
2.3. Assessment of Biomaterial Morphology
2.4. Evaluation of Specific Surface Area, Pore Distribution, and Pore Diameter of Biomaterials
2.5. Wound Fluid Uptake Test
2.6. Mechanical Properties
2.7. Water Vapor Transmission Test
2.8. Calcium Ion-Releasing Ability
2.9. Cell Culture Experiments
2.9.1. Cell Viability
2.9.2. Cell Proliferation
2.10. Statistical Analysis
3. Results and Discussion
3.1. Morphology of Biomaterials
3.2. Specific Surface Area, Pore Distribution, and Pore Diameter of Biomaterials
3.3. Ability of Biomaterials to Swell in Wound Fluid
3.4. Mechanical Properties of Biomaterials
3.5. Water Vapor Permeability of Biomaterials
3.6. Ability of Biomaterials to Release Calcium Ions
3.7. Fibroblast Viability
3.8. Fibroblast Proliferation
4. Conclusions
5. Patents
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Parameter | Cur_5% | Cur_8% | Cur_11% |
---|---|---|---|
Specific surface area * [m2/g] × 10−2 | 100 | 79.04 | 50.13 |
Cumulative pore area # [m2/g] × 10−2 | 69.74 | 45.60 | 17.10 |
Average pore size $ [nm] | 14.25 | 27.30 | 47.95 |
Biomaterial | Young’s Modulus ± SD [MPa] | Tensile Strength ± SD [MPa] |
---|---|---|
Cur_5% | 0.179 ± 0.036 * | 0.029 ± 0.002 * |
Cur_8% | 0.189 ± 0.004 * | 0.033 ± 0.011 * |
Cur_11% | 0.296 ± 0.036 | 0.051 ± 0.008 |
Sample | Fold Increase in Cell Proliferation |
---|---|
Control extract | 0.79 |
Cur_11% extract | 1.16 |
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Nurzynska, A.; Klimek, K.; Palka, K.; Szajnecki, Ł.; Ginalska, G. Curdlan-Based Hydrogels for Potential Application as Dressings for Promotion of Skin Wound Healing—Preliminary In Vitro Studies. Materials 2021, 14, 2344. https://doi.org/10.3390/ma14092344
Nurzynska A, Klimek K, Palka K, Szajnecki Ł, Ginalska G. Curdlan-Based Hydrogels for Potential Application as Dressings for Promotion of Skin Wound Healing—Preliminary In Vitro Studies. Materials. 2021; 14(9):2344. https://doi.org/10.3390/ma14092344
Chicago/Turabian StyleNurzynska, Aleksandra, Katarzyna Klimek, Krzysztof Palka, Łukasz Szajnecki, and Grazyna Ginalska. 2021. "Curdlan-Based Hydrogels for Potential Application as Dressings for Promotion of Skin Wound Healing—Preliminary In Vitro Studies" Materials 14, no. 9: 2344. https://doi.org/10.3390/ma14092344