Chitosan/Silver Nanoparticle/Graphene Oxide Nanocomposites with Multi-Drug Release, Antimicrobial, and Photothermal Conversion Functions
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Fabrication CS/RB-AgNPs/GO Nanocomposite
2.3. Materials Characterization
2.4. Drug Releasing Tests
2.5. Antibacterial Tests
2.6. Statistical Analysis
2.7. Photothermal Conversion Tests
3. Results
3.1. Materials Characterization
3.2. Microstructural and Mechanical Analysis
3.3. In-Vitro Drug Release Profiles
3.4. In-Vitro Drug Release Kinetic Model
3.5. Antibacterial Ability
3.6. Photothermal Conversion
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
- Tao, F.; Cheng, Y.; Shi, X.; Zheng, H.; Du, Y.; Xiang, W.; Deng, H. Applications of chitin and chitosan nanofibers in bone regenerative engineering. Carbohydr. Polym. 2020, 230, 115658. [Google Scholar] [CrossRef]
- Moeini, A.; Pedram, P.; Makvandi, P.; Malinconico, M.; d’Ayala, G. Wound healing and antimicrobial effect of active secondary metabolites in chitosan-based wound dressings: A review. Carbohydr. Polym. 2020, 233, 115839. [Google Scholar] [PubMed]
- Xu, K.L.; Ganapathy, K.; Andl, T.; Wang, Z.; Copland, J.A.; Chakrabarti, R.; Florczyk, S.J. 3D porous chitosan-alginate scaffold stiffness promotes differential responses in prostate cancer cell lines. Biomaterials 2019, 217, 12. [Google Scholar] [CrossRef]
- Su, Z.; Wang, H.; Tian, K.; Xu, F.; Huang, W.; Tian, X. Simultaneous reduction and surface functionalization of graphene oxide with wrinkled structure by diethylenetriamine (DETA) and their reinforcing effects in the flexible poly(2-ethylhexyl acrylate) (P2EHA) films. Compos. Part A Appl. Sci. Manuf. 2016, 84, 64–75. [Google Scholar] [CrossRef]
- Park, S.; Ruoff, R.S. Chemical methods for the production of graphenes. Nat. Nanotechnol. 2009, 4, 217–224. [Google Scholar] [CrossRef]
- Shin, S.R.; Li, Y.C.; Jang, H.L.; Khoshakhlagh, P.; Akbari, M.; Nasajpour, A.; Zhang, Y.S.; Tamayol, A.; Khademhosseini, A. Graphene-based materials for tissue engineering. Adv. Drug Deliv. Rev. 2016, 105, 255–274. [Google Scholar] [CrossRef] [Green Version]
- Jiang, Y.; Yang, Y.; Zheng, X.; Yi, Y.; Chen, X.; Li, Y.; Sun, D.; Zhang, L. Multifunctional load-bearing hybrid hydrogel with combined drug release and photothermal conversion functions. NPG Asia Mater. 2020, 12, 18. [Google Scholar] [CrossRef]
- Bai, Y.; Zhang, J.; Wen, D.; Gong, P.; Chen, X. A poly (vinyl butyral)/graphene oxide composite with NIR light-induced shape memory effect and solid-state plasticity. Compos. Sci. Technol. 2019, 170, 101–108. [Google Scholar] [CrossRef]
- Kalbacova, M.; Broz, A.; Kong, J.; Kalbac, M. Graphene substrates promote adherence of human osteoblasts and mesenchymal stromal cells. Carbon 2010, 48, 4323–4329. [Google Scholar] [CrossRef]
- Nayak, T.R.; Andersen, H.; Makam, V.S.; Khaw, C.; Bae, S.; Xu, X.; Ee, P.L.R.; Ahn, J.H.; Hong, B.H.; Pastorin, G.; et al. Graphene for controlled and accelerated osteogenic differentiation of human mesenchymal stem cells. ACS Nano 2011, 5, 4670–4678. [Google Scholar] [CrossRef] [Green Version]
- Barra, A.; Ferreira, N.M.; Martins, M.A.; Lazar, O.; Pantazi, A.; Jderu, A.A.; Neumayer, S.M.; Rodriguez, B.J.; Enăchescu, M.; Ferreira, P.; et al. Eco-friendly preparation of electrically conductive chitosan—Reduced graphene oxide flexible bionanocomposites for food packaging and biological applications. Compos. Sci. Technol. 2019, 173, 53–60. [Google Scholar] [CrossRef]
- Hermenean, A.; Codreanu, A.; Herman, H.; Balta, C.; Rosu, M.; Mihali, C.V.; Ivan, A.; Dinescu, S.; Ionita, M.; Costache, M. Chitosan-graphene oxide 3D scaffolds as promising tools for bone regeneration in critical-size mouse calvarial defects. Sci. Rep. 2017, 7, 16641. [Google Scholar]
- Rana, V.K.; Choi, M.-C.; Kong, J.-Y.; Kim, G.Y.; Kim, M.J.; Kim, S.-H.; Mishra, S.; Singh, R.P.; Ha, C.S. Synthesis and drug-delivery behavior of chitosan-functionalized graphene oxide hybrid nanosheets. Macromol. Mater. Eng. 2011, 296, 131–140. [Google Scholar] [CrossRef]
- Bao, H.; Pan, Y.; Ping, Y.; Sahoo, N.G.; Wu, T.; Li, L.; Li, J.; Gan, L.H. Chitosan-functionalized graphene oxide as a nanocarrier for drug and gene delivery. Small 2011, 7, 1569–1578. [Google Scholar] [CrossRef]
- Shamekhi, M.A.; Mirzadeh, H.; Mahdavi, H.; Rabiee, A.; Mohebbi-Kalhori, D.; Eslaminejad, M.B. Graphene oxide containing chitosan scaffolds for cartilage tissue engineering. Int. J. Biol. Macromol. 2019, 127, 396–405. [Google Scholar] [CrossRef] [PubMed]
- Liu, Y.; Fang, N.; Liu, B.; Song, L.; Wen, B.; Yang, D. Aligned porous chitosan/graphene oxide scaffold for bone tissue engineering. Mater. Lett. 2018, 233, 78–81. [Google Scholar] [CrossRef]
- Jiang, L.; Chen, D.; Wang, Z.; Zhang, Z.; Xia, Y.; Xue, H.; Lui, Y. Preparation of an electrically conductive graphene oxide/chitosan scaffold for cardiac tissue engineering. Appl. Biochem. Biotechnol. 2019, 188, 952–964. [Google Scholar] [CrossRef]
- Saravanan, S.; Sareen, N.; Abu-El-Rub, E.; Ashour, H.; Sequiera, G.L.; Ammar, H.I.; Gopinath, V.; Shamaa, A.A.; Sayed, S.S.E.; Moudgil, M.; et al. Graphene oxide-gold nanosheets containing chitosan scaffold improves ventricular contractility and function after implantation into infarcted heart. Sci. Rep. 2018, 8, 15069. [Google Scholar] [CrossRef]
- Khawaja, H.; Zahir, E.; Asghar, M.A.; Asghar, M.A. Graphene oxide, chitosan and silver nanocomposite as a highly effective antibacterial agent against pathogenic strains. Colloids Surf. A Physicochem. Eng. Asp. 2018, 555, 246–255. [Google Scholar] [CrossRef]
- Keshvardoostchokami, M.; Piri, F.; Jafarian, V.; Zamani, A. Fabrication and antibacterial properties of silver/graphite oxide/chitosan and silver/reduced graphene oxide/chitosan nanocomposites. JOM 2020, 72, 4477–4485. [Google Scholar] [CrossRef]
- Jena, G.; Anandkumar, B.; Vanithakumari, S.C.; George, R.P.; Philip, J.; Amarendra, G. Graphene oxide-chitosan-silver composite coating on Cu-Ni alloy with enhanced anticorrosive and antibacterial properties suitable for marine applications. Prog. Org. Coat. 2020, 139, 105444. [Google Scholar] [CrossRef]
- Sun, D.; McLaughlan, J.; Zhang, L.; Falzon, B.G.; Mariotti, D.; Maguire, P.; Sun, D. Atmospheric pressure plasma-synthesized gold nanoparticle/carbon nanotube hybrids for photothermal conversion. Langmuir 2019, 35, 4577–4588. [Google Scholar] [CrossRef] [PubMed]
- Nolan, H.; Sun, D.; Falzon, B.G.; Maguire, P.; Mariotti, D.; Zhang, L.; Sun, D. Thermoresponsive nanocomposites incorporating microplasma synthesized magnetic nanoparticles—Synthesis and potential applications. Plasma Process. Polym. 2019, 16, 1800128. [Google Scholar] [CrossRef] [Green Version]
- Ding, F.; Wang, Z.; He, S.; Shalaev, V.M.; Kildishev, A.V. Broadband high-efficiency half-wave plate: A supercell-based plasmonic metasurface approach. Acs Nano 2015, 9, 4111–4119. [Google Scholar] [CrossRef] [PubMed]
- Nolan, H.; Sun, D.; Falzon, B.G.; Chakrabarti, S.; Padmanaba, D.B.; Maguire, P.; Mariotti, D.; Yu, T.; Jones, D.; Andrews, G.; et al. Metal nanoparticle-hydrogel nanocomposites for biomedical applications—An atmospheric pressure plasma synthesis approach. Plasma Process. Polym. 2018, 15, 1800112. [Google Scholar] [CrossRef] [Green Version]
- Sun, D.; Turner, J.; Jiang, N.; Zhu, S.; Zhang, L.; Falzon, B.G.; McCoy, C.P.; Maguire, P.; Mariotti, D.; Sun, D. Atmospheric pressure microplasma for antibacterial silver nanoparticle/chitosan nanocomposites with tailored properties. Compos. Sci. Technol. 2020, 186, 107911. [Google Scholar] [CrossRef]
- Standard Test Method for Compressive Properties of Rigid Cellular Plastics, ASTM D1621—10; ASTM International: West Conshohocken, PA, USA, 2010.
- Karageorgiou, V.; Kaplan, D. Porosity of 3D biomaterial scaffolds and osteogenesis. Biomaterials 2005, 26, 5474–5491. [Google Scholar] [CrossRef] [PubMed]
- Guo, Z.J.; Jiang, N.; Moore, J.; McCoy, C.P.; Ziminska, M.; Rafferty, C.; Sarri, G.; Hamilton, A.R.; Li, Y.; Zhang, L.; et al. Nanoscale hybrid coating enables multifunctional tissue scaffold for potential multimodal therapeutic applications. Acs Appl. Mater. Interfaces 2019, 11, 27269–27278. [Google Scholar] [CrossRef]
- Hu, X.; Ren, N.; Chao, Y.; Lan, H.; Yan, X.; Sha, Y.; Sha, X.; Bai, Y. Highly aligned graphene oxide/poly(vinyl alcohol) nanocomposite fibers with high-strength, antiultraviolet and antibacterial properties. Compos. Part A Appl. Sci. Manuf. 2017, 102, 297–304. [Google Scholar] [CrossRef]
- He, M.; Zhu, C.; Xu, H.; Sun, D.; Chen, C.; Feng, G.; Liu, L.; Li, Y.; Zhang, L. Conducting polyetheretherketone nanocomposites with an electrophoretically deposited bioactive coating for bone tissue regeneration and multimodal therapeutic applications. ACS Appl. Mater. Interfaces 2020, 12, 56924–56934. [Google Scholar] [CrossRef]
- Chen, Y.; Chen, L.; Bai, H.; Li, L. Graphene oxide–chitosan composite hydrogels as broad-spectrum adsorbents for water purification. J. Mater. Chem. A 2013, 1, 1992–2001. [Google Scholar] [CrossRef]
- Yang, X.; Tu, Y.; Li, L.; Shang, S.; Tao, X.-M. Well-dispersed chitosan/graphene oxide nanocomposites. ACS Appl. Mater. Interfaces 2010, 2, 1707–1713. [Google Scholar] [CrossRef]
- Li, J.; Ren, N.; Qiu, J.; Mou, X.; Liu, H. Graphene oxide-reinforced biodegradable genipin-cross-linked chitosan fluorescent biocomposite film and its cytocompatibility. Int. J. Nanomed. 2013, 8, 3415. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Li, Y.; Jiang, L. Preparation of graphene oxide–chitosan nanocapsules and their applications as carriers for drug delivery. RSC Adv. 2016, 6, 104522–104528. [Google Scholar] [CrossRef]
- Marcano, D.C.; Kosynkin, D.V.; Berlin, J.M.; Sinitskii, A.; Sun, Z.; Slesarev, A.; Alemany, L.B.; Lu, W.; Tour, J.M. Improved synthesis of graphene oxide. ACS Nano 2010, 4, 4806–4814. [Google Scholar] [CrossRef] [PubMed]
- Su, Z.; Wang, H.; Tian, K.; Huang, W.; Guo, Y.; He, J.; Tian, X. Multifunctional anisotropic flexible cycloaliphatic epoxy resin nanocomposites reinforced by aligned graphite flake with non-covalent biomimetic functionalization. Compos. Part A Appl. Sci. Manuf. 2018, 109, 472–480. [Google Scholar] [CrossRef]
- Suyatma, N.E.; Tighzert, L.; Copinet, A.; Coma, V. Effects of hydrophilic plasticizers on mechanical, thermal, and surface properties of chitosan films. J. Agric. Food Chem. 2005, 53, 3950–3957. [Google Scholar] [CrossRef]
- Leszczynska, A.; Pielichowski, K. Application of thermal analysis methods for characterization of polymer/montmorillonite nanocomposites. J. Therm. Anal. Calorim. 2008, 93, 677–687. [Google Scholar] [CrossRef]
- Martini, B.; Dimida, S.; Benedetto, E.D.; Madaghiele, M.; Demitri, C. Study on the degradation of chitosan slurries. Results Phys. 2016, 6, 728–729. [Google Scholar] [CrossRef] [Green Version]
- Zou, J.; Kim, F. Self-assembly of two-dimensional nanosheets induced by interfacial polyionic complexation. ACS Nano 2012, 6, 10606–10613. [Google Scholar] [CrossRef]
- Yao, Z.; Braidy, N.; Botton, G.A.; Adronov, A. Polymerization from the surface of single-walled carbon nanotubes—Preparation and characterization of nanocomposites. J. Am. Chem. Soc. 2003, 125, 16015–16024. [Google Scholar] [CrossRef]
- González-Campos, J.B.; Mota-Morales, J.D.; Kumar, S.; Zárate-Triviño, D.; Hernández-Iturriaga, M.; Prokhorov, Y.; Lepe, M.V.; García-Carvajal, Z.Y.; Sanchez, I.C.; Luna-Bárcenas, G. New insights into the bactericidal activity of chitosan-Ag bionanocomposite: The role of the electrical conductivity. Colloids Surf. B Biointerfaces 2013, 111, 741–746. [Google Scholar] [CrossRef]
- Xu, K.; Liu, C.; Kang, K.; Zheng, Z.; Wang, S.; Tang, Z.; Yang, W. Isolation of nanocrystalline cellulose from rice straw and preparation of its biocomposites with chitosan: Physicochemical characterization and evaluation of interfacial compatibility. Compos. Sci. Technol. 2018, 154, 8–17. [Google Scholar] [CrossRef]
- Jin, L.; Bai, R. Mechanisms of lead adsorption on chitosan/PVA hydrogel beads. Langmuir 2002, 18, 9765–9770. [Google Scholar] [CrossRef]
- Hasan, A.; Waibhaw, G.; Saxena, V.; Pandey, L.M. Nano-biocomposite scaffolds of chitosan, carboxymethyl cellulose and silver nanoparticle modified cellulose nanowhiskers for bone tissue engineering applications. Int. J. Biol. Macromol. 2018, 111, 923–934. [Google Scholar] [CrossRef]
- Jiang, N.; Tan, P.; He, M.; Zhang, L.; Zhu, S. Graphene reinforced polyether ether ketone nanocomposites for bone repair applications. Polym. Test. 2020. (under revisions). [Google Scholar] [CrossRef]
- Kim, Y.-H.; Tabata, Y. Dual-controlled release system of drugs for bone regeneration. Adv. Drug Deliv. Rev. 2015, 94, 28–40. [Google Scholar]
- He, X.; Li, J.; An, S.; Jiang, C. pH-sensitive drug-delivery systems for tumor targeting. Ther. Deliv. 2013, 4, 1499–1510. [Google Scholar] [CrossRef] [PubMed]
- Tiwari, H.; Karki, N.; Pal, M.; Basak, S.; Verma, R.K.; Bal, R.; Kandpal, N.D.; Bisht, G.; Sahoo, N.G. Functionalized graphene oxide as a nanocarrier for dual drug delivery applications: The synergistic effect of quercetin and gefitinib against ovarian cancer cells. Colloids Surf. B Biointerfaces 2019, 178, 452–459. [Google Scholar] [CrossRef] [PubMed]
- Tran, T.H.; Nguyen, H.T.; Pham, T.T.; Choi, J.Y.; Choi, H.-G.; Yong, C.S.; Kim, J.O. Development of a graphene oxide nanocarrier for dual-drug chemo-phototherapy to overcome drug resistance in cancer. ACS Appl. Mater. Interfaces 2015, 7, 28647–28655. [Google Scholar] [CrossRef]
- Hashemzadeh, H.; Raissi, H. Understanding loading, diffusion and releasing of doxorubicin and paclitaxel dual delivery in graphene and graphene oxide carriers as highly efficient drug delivery systems. Appl. Surf. Sci. 2020, 500, 144220. [Google Scholar] [CrossRef]
- Pei, X.; Zhu, Z.; Gan, Z.; Chen, J.; Zhang, X.; Cheng, X.; Wan, Q.; Wang, J. PEGylated nano-graphene oxide as a nanocarrier for delivering mixed anticancer drugs to improve anticancer activity. Sci. Rep. 2020, 10, 1–15. [Google Scholar] [CrossRef] [PubMed]
- Rizwan, M.; Yahya, R.; Hassan, A.; Yar, M.; Azzahari, A.D.; Selvanathan, V.; Sonsudin, F.; Abouloula, C.N. Erratum: pH sensitive hydrogels in drug delivery: Brief history, properties, swelling, and release mechanism, material selection and applications. Polymers 2017, 9, 137. [Google Scholar] [CrossRef] [PubMed]
- 5—Mathematical Models of Drug Release. In Strategies to Modify the Drug Release from Pharmaceutical Systems; Bruschi, M.L. (Ed.) Woodhead Publishing: Cambridge, UK, 2015; pp. 63–86. [Google Scholar]
- Korsmeyer, R.W.; Gurny, R.; Doelker, E.; Buri, P.; Peppas, N.A. Mechanisms of solute release from porous hydrophilic polymers. Int. J. Pharm. 1983, 15, 25–35. [Google Scholar] [CrossRef]
- Peppas, N.A.; Korsmeyer, R. Dynamically swelling hydrogels in controlled release applications. Hydrogels Med. Pharm. 1987, 3, 109–136. [Google Scholar]
- Saurí, J.; Millán, D.; Suñé-Negre, J.M.; Colom, H.; Ticó, J.R.; Miñarro, M.; Pérez-Lozano, P.; García-Montoya, E. Quality by design approach to understand the physicochemical phenomena involved in controlled release of captopril SR matrix tablets. Int. J. Pharm. 2014, 477, 43–441. [Google Scholar] [CrossRef]
- Kevadiya, B.D.; Chettiar, S.S.; Rajkumar, S.; Bajaj, H.C.; Brahmbhatt, H.; Chaudhari, J.C.; Thumbar, R.P.; Jhala, D.; Rao, M.V. Evaluation of Montmorillonite/Poly (L-Lactide) microcomposite spheres as ambidextrous reservoirs for controlled release of Capecitabine (Xeloda) and assessment of cell cytotoxic and oxidative stress markers. Compos. Sci. Technol. 2014, 90, 193–201. [Google Scholar] [CrossRef]
- Higuchi, T. Rate of release of medicaments from ointment bases containing drugs in suspension. J. Pharm. Sci. 1961, 50, 874–875. [Google Scholar] [CrossRef]
- Farmoudeh, A.; Akbari, J.; Saeedi, M.; Ghasemi, M.; Asemi, N.; Nokhodchi, A. Methylene blue-loaded niosome: Preparation, physicochemical characterization, and in vivo wound healing assessment. Drug Deliv. Transl. Res. 2020, 10, 1428–1441. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Casalini, T.; Salvalaglio, M.; Perale, G.; Masi, M.; Cavallotti, C. Diffusion and aggregation of sodium fluorescein in aqueous solutions. J. Phys. Chem. B 2011, 115, 12896–12904. [Google Scholar] [CrossRef] [Green Version]
- Xing, K.; Fan, R.; Wang, F.; Nie, H.; Du, X.; Gai, S.; Wang, P.; Yang, Y. Dual-stimulus-triggered programmable drug release and luminescent ratiometric pH sensing from chemically stable biocompatible zinc metal–Organic framework. ACS Appl. Mater. Interfaces 2018, 10, 22746–22756. [Google Scholar] [CrossRef] [PubMed]
- Pape, H.L.; Solano-Serena, F.; Contini, P.; Devillers, C.; Maftah, A.; Leprat, P. Involvement of reactive oxygen species in the bactericidal activity of activated carbon fibre supporting silver: Bactericidal activity of ACF (Ag) mediated by ROS. J. Inorg. Biochem. 2004, 98, 1054–1060. [Google Scholar] [CrossRef]
- Qiu, J.; Geng, H.; Wang, D.; Qian, S.; Zhu, H.; Qiao, Y.; Qian, W.; Liu, X. Layer-number dependent antibacterial and osteogenic behaviors of graphene oxide electrophoretic deposited on titanium. ACS Appl. Mater. Interfaces 2017, 9, 12253–12263. [Google Scholar] [CrossRef] [PubMed]
- Ma, J.; Zhang, J.; Xiong, Z.; Yong, Y.; Zhao, X.S. Preparation, characterization and antibacterial properties of silver-modified graphene oxide. J. Mater. Chem. 2011, 21, 3350–3352. [Google Scholar] [CrossRef]
Sample Name | GO wt% (vs. CS) | AgNO3/CS Precursor Concentration | RT-APM |
---|---|---|---|
Pure CS | None | None | No |
CS/AgNPs | None | 4.0 mM AgNO3 | Yes |
CS/RB-AgNPs/GO-0.5 | 0.5% (w/w) | 4.0 mM AgNO3 | Yes |
CS/RB-AgNPs/GO-1.5 | 1.5% (w/w) | 4.0 mM AgNO3 | Yes |
Sample Code | Korsmeyer–Peppas Model Fit All Range | ||
---|---|---|---|
DK (×10−5 cm2s−1) | N | R2 | |
(CS/RB-AgNPs)-MB/GO-1.5 pH = 7.4 | 1.573 | 0.098 | 0.983 |
(CS/RB-AgNPs)-MB/GO-1.5 pH = 4.0 | 1.949 | 0.121 | 0.915 |
CS/RB-AgNPs/(GO-1.5)-FL pH = 7.4 | 0.0231 | 0.155 | 0.981 |
CS/RB-AgNPs/(GO-1.5)-FL pH = 4.0 | 0.0168 | 0.347 | 0.986 |
(CS/RB-AgNPs)-MB/(GO-1.5)-FL pH = 7.4 MB | 0.572 | 0.126 | 0.969 |
(CS/RB-AgNPs)-MB/(GO-1.5)-FL pH = 4.0 MB | 0.811 | 0.142 | 0.936 |
(CS/RB-AgNPs)-MB/(GO-1.5)-FL pH = 7.4 FL | 0.0437 | 0.161 | 0.995 |
(CS/RB-AgNPs)-MB/(GO-1.5)-FL pH = 4.0 FL | 0.0596 | 0.254 | 0.968 |
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Su, Z.; Sun, D.; Zhang, L.; He, M.; Jiang, Y.; Millar, B.; Douglas, P.; Mariotti, D.; Maguire, P.; Sun, D. Chitosan/Silver Nanoparticle/Graphene Oxide Nanocomposites with Multi-Drug Release, Antimicrobial, and Photothermal Conversion Functions. Materials 2021, 14, 2351. https://doi.org/10.3390/ma14092351
Su Z, Sun D, Zhang L, He M, Jiang Y, Millar B, Douglas P, Mariotti D, Maguire P, Sun D. Chitosan/Silver Nanoparticle/Graphene Oxide Nanocomposites with Multi-Drug Release, Antimicrobial, and Photothermal Conversion Functions. Materials. 2021; 14(9):2351. https://doi.org/10.3390/ma14092351
Chicago/Turabian StyleSu, Zheng, Daye Sun, Li Zhang, Miaomiao He, Yulin Jiang, Bronagh Millar, Paula Douglas, Davide Mariotti, Paul Maguire, and Dan Sun. 2021. "Chitosan/Silver Nanoparticle/Graphene Oxide Nanocomposites with Multi-Drug Release, Antimicrobial, and Photothermal Conversion Functions" Materials 14, no. 9: 2351. https://doi.org/10.3390/ma14092351