Assessment of the Impacts of Green Synthesized Silver Nanoparticles on Maerua oblongifolia Shoots under In Vitro Salt Stress
Abstract
:1. Introduction
2. Materials and Methods
2.1. Preparation of AgNPs Using Ochradenus Arabicus
2.2. Characterization of Green Synthesized Silver Nanoparticles
2.3. Plant Growth Conditions
2.4. Growth Parameters
2.5. Photosynthetic Pigments
2.6. Estimation of Total Soluble Sugar and Proline Contents
2.7. Lipid Peroxidation and Hydrogen Peroxide
2.8. Measurement of Antioxidant Enzyme Activities
2.9. Estimation of Nonenzymatic Antioxidants
2.10. Statistical Analyses
3. Results
3.1. Characterization of AgNPs Nanoparticles
3.2. Plant Growth Traits and Biomass Yield
3.3. Photosynthetic Pigments
3.4. Proline and Soluble Sugar Contents
3.5. Lipid Peroxidation and Hydrogen Peroxide Contents
3.6. Antioxidant Enzyme Activities
3.7. Nonenzymatic Antioxidants
4. Discussion
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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NaCl × AgNPs | Fresh Weight (g) | Dry Weight (g) | Shoot Number (pot) | Shoot Length (cm) | |
---|---|---|---|---|---|
0 NaCl | 0 mg/L NPs | 7.06 ± 0.15 j | 1.40 ± 0.17 e | 11.66 ± 0.57 g | 6.46 ± 0.05 h |
10 mg/L NPs | 16.9 ± 0.10 c | 3.2 ± 0.20 c | 20.5 ± 0.20 b | 13.3 ± 0.15 b | |
20 mg/L NPs | 18.2 ± 0.15 a | 4.3 ± 0.15 a | 21.2 ± 0.11 a | 14.2 ± 0.15 a | |
30 mg/L NPs | 17.2 ± 0.15 b | 4.1 ± 0.20 a | 21.4 ± 0.30 a | 13.8 ± 0.10 b | |
50 NaCl | 0 mg/L NPs | 6.30 ± 0.20 k | 1.06 ± 0.05 f | 10.66 ± 0.57 h | 5.93 ± 0.10 i |
10 mg/L NPs | 15.3 ± 0.10 de | 3.73 ± 0.05 b | 20.66 ± 0.57 b | 12.73 ± 0.05 c | |
20 mg/L NPs | 15.53 ± 0.30 d | 3.63 ± 0.10 b | 20.66 ± 0.57 b | 12.87 ± 0.05 c | |
30 mg/L NPs | 14.86 ± 0.15 e | 3.36 ± 0.05 c | 18.33 ± 0.57 d | 12.43 ± 0.05 cd | |
100 NaCl | 0 mg/L NPs | 5.66 ± 0.15 l | 0.90 ± 0.11 fg | 10.33 ± 0.57 h | 4.1 ± 0.05 h |
10 mg/L NPs | 14.76 ± 0.15 e | 3.33 ± 0.10 c | 19.33 ± 0.57 c | 11.23 ± 0.05 e | |
20 mg/L NPs | 15.3 ± 0.05 de | 3.70 ± 0.10 b | 20.66 ± 0.57 b | 12.63 ± 0.05 c | |
30 mg/L NPs | 14.23 ± 0.05 f | 3.10 ± 0.23 c | 18.33 ± 0.57 d | 12.46 ± 0.05 cd | |
200 NaCl | 0 mg/L NPs | 5.03 ± 0.15 m | 0.73 ± 0.05 g | 9.33 ± 0.57 i | 3.86 ± 0.05 j |
10 mg/L NPs | 7.96 ± 0.15 i | 1.43 ± 0.15 e | 11.33 ± 0.57 f | 6.96 ± 0.05 h | |
20 mg/L NPs | 8.53 ± 0.15 h | 1.8 ± 0.10 d | 11.66 ± 0.57 f | 8.433 ± 0.05 g | |
30 mg/L NPs | 11.06 ± 0.25 g | 1.96 ± 0.05 d | 15.33 ± 0.75 e | 9.267 ± 0.11 f |
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Shaikhaldein, H.O.; Al-Qurainy, F.; Nadeem, M.; Khan, S.; Tarroum, M.; Salih, A.M.; Alansi, S.; Al-Hashimi, A.; Alfagham, A.; Alkahtani, J. Assessment of the Impacts of Green Synthesized Silver Nanoparticles on Maerua oblongifolia Shoots under In Vitro Salt Stress. Materials 2022, 15, 4784. https://doi.org/10.3390/ma15144784
Shaikhaldein HO, Al-Qurainy F, Nadeem M, Khan S, Tarroum M, Salih AM, Alansi S, Al-Hashimi A, Alfagham A, Alkahtani J. Assessment of the Impacts of Green Synthesized Silver Nanoparticles on Maerua oblongifolia Shoots under In Vitro Salt Stress. Materials. 2022; 15(14):4784. https://doi.org/10.3390/ma15144784
Chicago/Turabian StyleShaikhaldein, Hassan O., Fahad Al-Qurainy, Mohammad Nadeem, Salim Khan, Mohamed Tarroum, Abdalrhaman M. Salih, Saleh Alansi, Abdulrahman Al-Hashimi, Alanoud Alfagham, and Jawaher Alkahtani. 2022. "Assessment of the Impacts of Green Synthesized Silver Nanoparticles on Maerua oblongifolia Shoots under In Vitro Salt Stress" Materials 15, no. 14: 4784. https://doi.org/10.3390/ma15144784
APA StyleShaikhaldein, H. O., Al-Qurainy, F., Nadeem, M., Khan, S., Tarroum, M., Salih, A. M., Alansi, S., Al-Hashimi, A., Alfagham, A., & Alkahtani, J. (2022). Assessment of the Impacts of Green Synthesized Silver Nanoparticles on Maerua oblongifolia Shoots under In Vitro Salt Stress. Materials, 15(14), 4784. https://doi.org/10.3390/ma15144784