Nanophotosensitizers Composed of Phenyl Boronic Acid Pinacol Ester-Conjugated Chitosan Oligosaccharide via Thioketal Linker for Reactive Oxygen Species-Sensitive Delivery of Chlorin e6 against Oral Cancer Cells
Abstract
:1. Introduction
2. Materials and Methods
2.1. Chemicals
2.2. Synthesis of COSthPBAP Conjugates
2.3. Characterization of COSthPBAP Conjugates
2.4. Fabrication of Ce6-Incorporated Nanophotosensitizers
2.5. Characterization of Nanophotosensitizers
2.6. Drug Release Study
2.7. Devices for Light Irradiation
2.8. Singlet Oxygen (SO) Generation of Nanophotosensitizers
2.9. Cell Culture and Culture Media
2.10. PDT of Oral Cancer Cells In Vitro
2.11. Intracellular Ce6 Uptake and ROS Generation of Oral Cancer Cells In Vitro
2.12. Animal Tumor Imaging and In Vivo PDT Efficacy against AT84 Tumor Xenograft Model
2.13. Statistical Analysis
3. Results
3.1. Synthesis and Characterization of COSthPBAP Copolymer
3.2. Characterization of Ce6-Incorporated COSthPBAP Nanophotosensitizers
3.3. Cell Culture Study In Vitro
3.4. Animal Tumor Imaging of Tumor Xenograft Model
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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COSthPBAP/Ce6 Weight (mg/mg) | Drug Contents (%, w/w) 1 | Loading Efficiency (%, w/w) 1 | Particle Size (nm) 2 | Polydispersity | Zeta Potential (mV) 3 |
---|---|---|---|---|---|
40/0 | - | - | 84.4 ± 4.5 | 0.093 | 8.2 ± 0.35 |
40/2 | 4.6 | 96.0 | 132.9 ± 5.2 | 0.111 | 4.6 ± 0.19 |
40/4 | 8.5 | 93.0 | 146.2 ± 4.1 | 0.107 | 3.1 ± 0.23 |
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Hong, S.-O.; Kook, M.-S.; Jeong, Y.-I.; Park, M.-J.; Yang, S.-W.; Kim, B.-H. Nanophotosensitizers Composed of Phenyl Boronic Acid Pinacol Ester-Conjugated Chitosan Oligosaccharide via Thioketal Linker for Reactive Oxygen Species-Sensitive Delivery of Chlorin e6 against Oral Cancer Cells. Materials 2022, 15, 7057. https://doi.org/10.3390/ma15207057
Hong S-O, Kook M-S, Jeong Y-I, Park M-J, Yang S-W, Kim B-H. Nanophotosensitizers Composed of Phenyl Boronic Acid Pinacol Ester-Conjugated Chitosan Oligosaccharide via Thioketal Linker for Reactive Oxygen Species-Sensitive Delivery of Chlorin e6 against Oral Cancer Cells. Materials. 2022; 15(20):7057. https://doi.org/10.3390/ma15207057
Chicago/Turabian StyleHong, Sung-Ok, Min-Suk Kook, Young-IL Jeong, Min-Ju Park, Seong-Won Yang, and Byung-Hoon Kim. 2022. "Nanophotosensitizers Composed of Phenyl Boronic Acid Pinacol Ester-Conjugated Chitosan Oligosaccharide via Thioketal Linker for Reactive Oxygen Species-Sensitive Delivery of Chlorin e6 against Oral Cancer Cells" Materials 15, no. 20: 7057. https://doi.org/10.3390/ma15207057