Preparation of Collagen/Hydroxyapatite Composites Using the Alternate Immersion Method and Evaluation of the Cranial Bone-Forming Capability of Composites Complexed with Acidic Gelatin and b-FGF

Hoshi, Miki; Taira, Masayuki; Sawada, Tomofumi; Hachinohe, Yuki; Hatakeyama, Wataru; Takafuji, Kyoko; Tekemoto, Shinji; Kondo, Hisatomo

doi:10.3390/ma15248802

Open AccessArticle

Preparation of Collagen/Hydroxyapatite Composites Using the Alternate Immersion Method and Evaluation of the Cranial Bone-Forming Capability of Composites Complexed with Acidic Gelatin and b-FGF

by

Miki Hoshi

¹,

Masayuki Taira

^2,*,

Tomofumi Sawada

²

,

Yuki Hachinohe

¹,

Wataru Hatakeyama

¹,

Kyoko Takafuji

¹,

Shinji Tekemoto

²

and

Hisatomo Kondo

¹

Department of Prosthodontics and Oral Implantology, School of Dentistry, Iwate Medical University, 19-1 Uchimaru, Morioka 020-8505, Japan

²

Department of Biomedical Engineering, Iwate Medical University, 1-1-1 Idaidori, Yahaba-cho 028-3694, Japan

^*

Author to whom correspondence should be addressed.

Materials 2022, 15(24), 8802; https://doi.org/10.3390/ma15248802

Submission received: 15 November 2022 / Revised: 4 December 2022 / Accepted: 6 December 2022 / Published: 9 December 2022

(This article belongs to the Special Issue Naturally Derived Biomaterials for Regenerative Medicine Applications)

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Abstract

Bone-substitute materials are essential in dental implantology. We prepared collagen (Col)/hydroxyapatite (Hap)/acidic gelatin (AG)/basic fibroblast growth factor (b-FGF) constructs with enhanced bone-forming capability. The Col/Hap apatite composites were prepared by immersing Col sponges alternately in calcium and phosphate ion solutions five times, for 20 and 60 min, respectively. Then, the sponges were heated to 56 °C for 48 h. Scanning electron microscopy/energy-dispersive X-ray spectroscopy, Fourier-transform infrared spectroscopy, and X-ray diffraction analyses showed that the Col/Hap composites contained poorly crystalline Hap precipitates on the Col matrix. Col/Hap composite granules were infiltrated by AG, freeze-dried, and immersed in b-FGF solution. The wet quaternary constructs were implanted in rat cranial bone defects for 8 weeks, followed by soft X-ray measurements and histological analysis. Animal studies have shown that the constructs moderately increase bone formation in cranial bone defects. We found that an alternate immersion time of 20 min led to the greatest bone formation (p < 0.05). Constructs placed inside defects slightly extend the preexisting bone from the defect edges and lead to the formation of small island-like bones inside the defect, followed by disappearance of the constructs. The combined use of Col, Hap, AG, and b-FGF might bring about novel bone-forming biomaterials.

Keywords: collagen/hydroxyapatite composite; acidic-gelatin; alternate immersion method; hydroxyapatite precipitation; basic-fibroblast growth factor; bone formation; bone histology; bone substitute materials; dental implantology

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MDPI and ACS Style

Hoshi, M.; Taira, M.; Sawada, T.; Hachinohe, Y.; Hatakeyama, W.; Takafuji, K.; Tekemoto, S.; Kondo, H. Preparation of Collagen/Hydroxyapatite Composites Using the Alternate Immersion Method and Evaluation of the Cranial Bone-Forming Capability of Composites Complexed with Acidic Gelatin and b-FGF. Materials 2022, 15, 8802. https://doi.org/10.3390/ma15248802

AMA Style

Hoshi M, Taira M, Sawada T, Hachinohe Y, Hatakeyama W, Takafuji K, Tekemoto S, Kondo H. Preparation of Collagen/Hydroxyapatite Composites Using the Alternate Immersion Method and Evaluation of the Cranial Bone-Forming Capability of Composites Complexed with Acidic Gelatin and b-FGF. Materials. 2022; 15(24):8802. https://doi.org/10.3390/ma15248802

Chicago/Turabian Style

Hoshi, Miki, Masayuki Taira, Tomofumi Sawada, Yuki Hachinohe, Wataru Hatakeyama, Kyoko Takafuji, Shinji Tekemoto, and Hisatomo Kondo. 2022. "Preparation of Collagen/Hydroxyapatite Composites Using the Alternate Immersion Method and Evaluation of the Cranial Bone-Forming Capability of Composites Complexed with Acidic Gelatin and b-FGF" Materials 15, no. 24: 8802. https://doi.org/10.3390/ma15248802

APA Style

Hoshi, M., Taira, M., Sawada, T., Hachinohe, Y., Hatakeyama, W., Takafuji, K., Tekemoto, S., & Kondo, H. (2022). Preparation of Collagen/Hydroxyapatite Composites Using the Alternate Immersion Method and Evaluation of the Cranial Bone-Forming Capability of Composites Complexed with Acidic Gelatin and b-FGF. Materials, 15(24), 8802. https://doi.org/10.3390/ma15248802

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Preparation of Collagen/Hydroxyapatite Composites Using the Alternate Immersion Method and Evaluation of the Cranial Bone-Forming Capability of Composites Complexed with Acidic Gelatin and b-FGF

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