Impact of APRF+ in Combination with Autogenous Fibroblasts on Release Growth Factors, Collagen, and Proliferation and Migration of Gingival Fibroblasts: An In Vitro Study
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Culture and A-PRF+-Based Matrices
2.2. Assessment of Growth Factor Release from Fibroblasts Alone, A-PRF+ Alone, and A-PRF+ with Fibroblasts
2.3. The Quantification of Growth Factors with Enzyme-Linked Immunosorbent Assay (ELISA)
2.4. Quantification of Soluble Collagen Using the Sircol™ Colorimetric Test
2.5. Preparation of the Conditioned Media
2.6. Cell Migration Assay
2.7. Cell Viability Assay
2.8. Statistical Analysis
3. Results
3.1. Growth Factor Release from A-PRF+ Alone, A-PRF+ with Fibroblasts, and Fibroblasts Alone
3.2. Influence of Proteins Released from A-PRF+ Combined with Fibroblasts on Cell Viability
3.3. Enhanced Wound Healing Potential of Primary Human Gingival Fibroblasts Induced with Proteins Released from A-PRF+
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Sterczała, B.; Chwiłkowska, A.; Szwedowicz, U.; Kobielarz, M.; Chwiłkowski, B.; Dominiak, M. Impact of APRF+ in Combination with Autogenous Fibroblasts on Release Growth Factors, Collagen, and Proliferation and Migration of Gingival Fibroblasts: An In Vitro Study. Materials 2022, 15, 796. https://doi.org/10.3390/ma15030796
Sterczała B, Chwiłkowska A, Szwedowicz U, Kobielarz M, Chwiłkowski B, Dominiak M. Impact of APRF+ in Combination with Autogenous Fibroblasts on Release Growth Factors, Collagen, and Proliferation and Migration of Gingival Fibroblasts: An In Vitro Study. Materials. 2022; 15(3):796. https://doi.org/10.3390/ma15030796
Chicago/Turabian StyleSterczała, Barbara, Agnieszka Chwiłkowska, Urszula Szwedowicz, Magdalena Kobielarz, Bartłomiej Chwiłkowski, and Marzena Dominiak. 2022. "Impact of APRF+ in Combination with Autogenous Fibroblasts on Release Growth Factors, Collagen, and Proliferation and Migration of Gingival Fibroblasts: An In Vitro Study" Materials 15, no. 3: 796. https://doi.org/10.3390/ma15030796