Nanocomposites of Graphene Oxide—Silver Nanoparticles for Enhanced Antibacterial Activity: Mechanism of Action and Medical Textiles Coating
Abstract
:1. Introduction
2. Materials and Methods
2.1. Bacterial Culture
2.2. Characterization of Nanoparticles
2.3. Agar Well Diffusion Method
2.4. Ultrastructural Analysis by Transmission Electron Microscopy (TEM)
2.5. Viability XTT Assay
2.6. ROS Production
2.7. Lipid Peroxidation (MDA) Assay
2.8. Textile Fabrics–Determination of Antibacterial Activity–Agar Diffusion Plate Test (ISO 20645:2004)
2.9. Chorioallantoic Membrane (CAM) Assay
2.10. Statistics
3. Results
3.1. Characterization of Nanoparticles
3.2. Agar Well Diffusion Method
3.3. Ultrastructural Analysis by TEM
3.4. Viability XTT Assay
3.5. ROS Production
3.6. Lipid Peroxidation (MDA) Assay
3.7. Textile Fabrics—Determination of Antibacterial Activity—Agar Diffusion Plate Test (ISO 20645:2004)
3.8. Chorioallantoic Membrane (CAM) Assay
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Lange, A.; Sawosz, E.; Wierzbicki, M.; Kutwin, M.; Daniluk, K.; Strojny, B.; Ostrowska, A.; Wójcik, B.; Łojkowski, M.; Gołębiewski, M.; et al. Nanocomposites of Graphene Oxide—Silver Nanoparticles for Enhanced Antibacterial Activity: Mechanism of Action and Medical Textiles Coating. Materials 2022, 15, 3122. https://doi.org/10.3390/ma15093122
Lange A, Sawosz E, Wierzbicki M, Kutwin M, Daniluk K, Strojny B, Ostrowska A, Wójcik B, Łojkowski M, Gołębiewski M, et al. Nanocomposites of Graphene Oxide—Silver Nanoparticles for Enhanced Antibacterial Activity: Mechanism of Action and Medical Textiles Coating. Materials. 2022; 15(9):3122. https://doi.org/10.3390/ma15093122
Chicago/Turabian StyleLange, Agata, Ewa Sawosz, Mateusz Wierzbicki, Marta Kutwin, Karolina Daniluk, Barbara Strojny, Agnieszka Ostrowska, Barbara Wójcik, Maciej Łojkowski, Marcin Gołębiewski, and et al. 2022. "Nanocomposites of Graphene Oxide—Silver Nanoparticles for Enhanced Antibacterial Activity: Mechanism of Action and Medical Textiles Coating" Materials 15, no. 9: 3122. https://doi.org/10.3390/ma15093122