Evaluation of Cytotoxicity of Hyaluronic Acid/Chitosan/Bacterial Cellulose-Based Membrane
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Preparation of BC Membranes
2.3. Preparation of Chitosan-Modified Bacterial Cellulose (BC/CS) Membranes by Periodate Oxidation
2.4. Preparation of Chitosan–Alginate and Chitosan–Hyaluronic Acid Laminated BC Membranes Using the LBL Method
2.4.1. Preparation of Chitosan-Alginate Laminated Membranes
2.4.2. Preparation of Chitosan-Hyaluronic Acid-Laminated Membranes
2.5. Fourier-Transform Infrared (FT-IR) Spectroscopy
2.6. Thermogravimetric Analysis (TGA)
2.7. Scanning Electron Microscopy (SEM)
2.8. Thickness
2.9. Tensile Properties
2.10. Cytotoxicity Evaluation
2.10.1. Cell Propagation for Cytotoxicity Test
2.10.2. Cytotoxicity Test
3. Results and Discussion
3.1. FT-IR Analysis
3.2. Thermal Analysis
3.3. Physical Appearance and Morphology of the Membrane
3.4. Thickness in the Swollen State, Moisture Content and Tensile Properties
3.5. Cytotoxicity Analysis
4. Conclusions
Author Contributions
Funding
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Sample Name | Coating Cycles |
---|---|
BC | - |
BC(CS/Alg)5 | 5 |
BC(CS/Alg)10 | 10 |
BC(CS/HA)5 | 5 |
BC(CS/HA)10 | 10 |
Sample Name | Thickness (mm) in the Swollen State | Thickness (mm) of Dried Membrane | Moisture Content (%) of Dried Membrane |
---|---|---|---|
BC | 0.49 ± 0.08 | 0.007 ± 0.004 | 3.20 ± 0.07 |
BC(CS/Alg)5 | 0.49 ± 0.06 | 0.042 ± 0.013 | 4.36 ± 0.13 |
BC(CS/Alg)10 | 0.34 ± 0.04 | 0.062 ± 0.022 | 4.73 ± 0.23 |
BC(CS/HA)5 | 0.30 ± 0.06 | 0.015 ± 0.005 | 3.99 ± 0.06 |
BC(CS/HA)10 | 0.32 ± 0.06 | 0.027 ± 0.015 | 4.25 ± 0.10 |
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Dechojarassri, D.; Okada, T.; Tamura, H.; Furuike, T. Evaluation of Cytotoxicity of Hyaluronic Acid/Chitosan/Bacterial Cellulose-Based Membrane. Materials 2023, 16, 5189. https://doi.org/10.3390/ma16145189
Dechojarassri D, Okada T, Tamura H, Furuike T. Evaluation of Cytotoxicity of Hyaluronic Acid/Chitosan/Bacterial Cellulose-Based Membrane. Materials. 2023; 16(14):5189. https://doi.org/10.3390/ma16145189
Chicago/Turabian StyleDechojarassri, Duangkamol, Tomoki Okada, Hiroshi Tamura, and Tetsuya Furuike. 2023. "Evaluation of Cytotoxicity of Hyaluronic Acid/Chitosan/Bacterial Cellulose-Based Membrane" Materials 16, no. 14: 5189. https://doi.org/10.3390/ma16145189