Correlating the Effect of Composition and Textural Properties on Bioactivity for Pristine and Copper-Doped Binary Mesoporous Bioactive Glass Nanoparticles
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Synthesis of MBGNs and Cu-MBGNs
2.3. Morphological and Structural Characterizations
2.4. In Vitro Bioactivity Study
2.5. Copper Release in SBF
3. Results and Discussion
3.1. MBGNs Morphological and Structural Characterizations
3.1.1. Particle Size and Morphology
3.1.2. Structural Studies
3.1.3. MBGNs and Cu-MBGNs Composition
3.1.4. Particles Textural Properties
3.1.5. Network Connectivity
3.2. In Vitro Bioactivity in SBF
3.2.1. Reactivity of Binary Bioactive Glass Nanoparticles (MBGNs)
3.2.2. Reactivity of Copper-Doped Bioactive Glass Nanoparticles (Cu-MBGNs)
3.2.3. Copper Release in SBF
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
- Jacobs, A.; Renaudin, G.; Charbonnel, N.; Nedelec, J.-M.; Forestier, C.; Descamps, S. Copper-Doped Biphasic Calcium Phosphate Powders: Dopant Release, Cytotoxicity and Antibacterial Properties. Materials 2021, 14, 2393. [Google Scholar] [CrossRef]
- Wu, C.; Zhou, Y.; Xu, M.; Han, P.; Chen, L.; Chang, J.; Xiao, Y. Copper-containing mesoporous bioactive glass scaffolds with multifunctional properties of angiogenesis capacity, osteostimulation and antibacterial activity. Biomaterials 2013, 34, 422–433. [Google Scholar] [CrossRef] [PubMed]
- Zheng, K.; Dai, X.; Lu, M.; Hüser, N.; Taccardi, N.; Boccaccini, A.R. Synthesis of copper-containing bioactive glass nanoparticles using a modified Stöber method for biomedical applications. Colloids Surf. B Biointerfaces 2017, 150, 159–167. [Google Scholar] [CrossRef] [PubMed]
- Westhauser, F.; Wilkesmann, S.; Nawaz, Q.; Hohenbild, F.; Rehder, F.; Saur, M.; Fellenberg, J.; Moghaddam, A.; Ali, M.S.; Peukert, W.; et al. Effect of manganese, zinc, and copper on the biological and osteogenic properties of mesoporous bioactive glass nanoparticles. J. Biomed. Mater. Res. Part A 2020, 109, 1457–1467. [Google Scholar] [CrossRef] [PubMed]
- Bari, A.; Bloise, N.; Fiorilli, S.; Novajra, G.; Vallet-Regí, M.; Bruni, G.; Torres-Pardo, A.; González-Calbet, J.M.; Visai, L.; Vitale-Brovarone, C. Copper-containing mesoporous bioactive glass nanoparticles as multifunctional agent for bone regeneration. Acta Biomater. 2017, 55, 493–504. [Google Scholar] [CrossRef]
- Hosseini, M.; Besheli, N.H.; Deng, D.; Lievens, C.; Zuo, Y.; Leeuwenburgh, S.C.; Yang, F. Facile post modification synthesis of copper-doped mesoporous bioactive glass with high antibacterial performance to fight bone infection. Mater. Sci. Eng. C 2023, 144, 213198. [Google Scholar] [CrossRef] [PubMed]
- Kokubo, T.; Takadama, H. How useful is SBF in predicting in vivo bone bioactivity? Biomaterials 2006, 27, 2907–2915. [Google Scholar] [CrossRef]
- Kesse, X.; Vichery, C.; Nedelec, J.-M. Deeper Insights into a Bioactive Glass Nanoparticle Synthesis Protocol to Control Its Morphology, Dispersibility, and Composition. ACS Omega 2019, 4, 5768–5775. [Google Scholar] [CrossRef]
- Zheng, K.; Sui, B.; Ilyas, K.; Boccaccini, A.R. Porous bioactive glass micro- and nanospheres with controlled morphology: Developments, properties and emerging biomedical applications. Mater. Horiz. 2020, 8, 300–335. [Google Scholar] [CrossRef]
- De Oliveira, A.A.R.; De Souza, D.A.; Dias, L.L.S.; De Carvalho, S.M.; Mansur, H.S.; Pereira, M.D.M. Synthesis, characterization and cytocompatibility of spherical bioactive glass nanoparticles for potential hard tissue engineering applications. Biomed. Mater. 2013, 8, 025011. [Google Scholar] [CrossRef]
- Li, R.; Clark, A.E.; Hench, L.L. An investigation of bioactive glass powders by sol-gel processing. J. Appl. Biomater. 1991, 2, 231–239. [Google Scholar] [CrossRef]
- Martínez, A.; Izquierdo-Barba, I.; Vallet-Regí, M. Bioactivity of a CaO−SiO2 Binary Glasses System. Chem. Mater. 2000, 12, 3080–3088. [Google Scholar] [CrossRef]
- Saravanapavan, P.; Hench, L.L. Low-temperature synthesis, structure, and bioactivity of gel-derived glasses in the binary CaO-SiO2 system. J Biomed Mater Res. 2001, 54, 608–618. [Google Scholar] [CrossRef]
- Kesse, X.; Vichery, C.; Jacobs, A.; Descamps, S.; Nedelec, J.-M. Unravelling the Impact of Calcium Content on the Bioactivity of Sol–Gel-Derived Bioactive Glass Nanoparticles. ACS Appl. Bio Mater. 2020, 3, 1312–1320. [Google Scholar] [CrossRef] [PubMed]
- Rahaman, M.N.; Day, D.E.; Bal, B.S.; Fu, Q.; Jung, S.B.; Bonewald, L.F.; Tomsia, A.P. Bioactive glass in tissue engineering. Acta Biomater. 2011, 7, 2355–2373. [Google Scholar] [CrossRef]
- Cacciotti, I. Bivalent cationic ions doped bioactive glasses: The influence of magnesium, zinc, strontium and copper on the physical and biological properties. J. Mater. Sci. 2017, 52, 8812–8831. [Google Scholar] [CrossRef]
- Kargozar, S.; Mozafari, M.; Ghodrat, S.; Fiume, E.; Baino, F. Copper-containing bioactive glasses and glass-ceramics: From tissue regeneration to cancer therapeutic strategies. Mater. Sci. Eng. C 2020, 121, 111741. [Google Scholar] [CrossRef] [PubMed]
- Bejarano, J.; Caviedes, P.; Palza, H. Sol–gel synthesis and in vitro bioactivity of copper and zinc-doped silicate bioactive glasses and glass-ceramics. Biomed. Mater. 2015, 10, 025001. [Google Scholar] [CrossRef] [PubMed]
- Baino, F. Copper-Doped Ordered Mesoporous Bioactive Glass: A Promising Multifunctional Platform for Bone Tissue Engineering. Bioengineering 2020, 7, 45. [Google Scholar] [CrossRef]
- Kesse, X.; Adam, A.; Begin-Colin, S.; Mertz, D.; Larquet, E.; Gacoin, T.; Maurin, I.; Vichery, C.; Nedelec, J.-M. Elaboration of Superparamagnetic and Bioactive Multicore–Shell Nanoparticles (γ-Fe2O3@SiO2-CaO): A Promising Material for Bone Cancer Treatment. ACS Appl. Mater. Interfaces 2020, 12, 47820–47830. [Google Scholar] [CrossRef]
- Yun, H.-S.; Kim, S.-H.; Lee, S.; Song, I.-H. Synthesis of high surface area mesoporous bioactive glass nanospheres. Mater. Lett. 2010, 64, 1850–1853. [Google Scholar] [CrossRef]
- Aguiar, H.; Serra, J.; González, P.; León, B. Structural study of sol–gel silicate glasses by IR and Raman spectroscopies. J. Non-Cryst. Solids 2009, 355, 475–480. [Google Scholar] [CrossRef]
- Greasley, S.L.; Page, S.J.; Sirovica, S.; Chen, S.; Martin, R.A.; Riveiro, A.; Hanna, J.V.; Porter, A.E.; Jones, J.R. Controlling particle size in the Stöber process and incorporation of calcium. J. Colloid Interface Sci. 2016, 469, 213–223. [Google Scholar] [CrossRef]
- Taghvaei, A.H.; Danaeifar, F.; Gammer, C.; Eckert, J.; Khosravimelal, S.; Gholipourmalekabadi, M. Synthesis and characterization of novel mesoporous strontium-modified bioactive glass nanospheres for bone tissue engineering applications. Microporous Mesoporous Mater. 2019, 294, 109889. [Google Scholar] [CrossRef]
- Zhao, S.; Li, Y.; Li, D. Synthesis and in vitro bioactivity of CaO–SiO2–P2O5 mesoporous microspheres. Microporous Mesoporous Mater. 2010, 135, 67–73. [Google Scholar] [CrossRef]
- Kurtuldu, F.; Kaňková, H.; Beltrán, A.M.; Liverani, L.; Galusek, D.; Boccaccini, A.R. Anti-inflammatory and antibacterial activities of cerium-containing mesoporous bioactive glass nanoparticles for drug-free biomedical applications. Mater. Today Bio 2021, 12, 100150. [Google Scholar] [CrossRef]
- Li, X.; Zhang, L.; Dong, X.; Liang, J.; Shi, J. Preparation of mesoporous calcium doped silica spheres with narrow size dispersion and their drug loading and degradation behavior. Microporous Mesoporous Mater. 2007, 102, 151–158. [Google Scholar] [CrossRef]
- Li, Y.; Chen, X.; Ning, C.; Yuan, B.; Hu, Q. Facile synthesis of mesoporous bioactive glasses with controlled shapes. Mater. Lett. 2015, 161, 605–608. [Google Scholar] [CrossRef]
- Min, Z.; Huixue, W.; Yujie, Z.; Lixin, J.; Hai, H.; Yufang, Z. Synthesis of monodispersed mesoporous bioactive glass nanospheres for bone repair. Mater. Lett. 2016, 171, 259–262. [Google Scholar] [CrossRef]
- Leonova, E.; Izquierdo-Barba, I.; Arcos, D.; López-Noriega, A.; Hedin, N.; Vallet-Regí, M.; Edén, M. Multinuclear Solid-State NMR Studies of Ordered Mesoporous Bioactive Glasses. J. Phys. Chem. C 2008, 112, 5552–5562. [Google Scholar] [CrossRef]
- Lin, S.; Ionescu, C.; Pike, K.J.; Smith, M.E.; Jones, J.R. Nanostructure evolution and calcium distribution in sol–gel derived bioactive glass. J. Mater. Chem. 2009, 19, 1276–1282. [Google Scholar] [CrossRef]
- Mokhtari, S.; Skelly, K.D.; Krull, E.A.; Coughlan, A.; Mellott, N.P.; Gong, Y.; Borges, R.; Wren, A.W. Copper-containing glass polyalkenoate cements based on SiO2–ZnO–CaO–SrO–P2O5 glasses: Glass characterization, physical and antibacterial properties. J. Mater. Sci. 2017, 52, 8886–8903. [Google Scholar] [CrossRef]
- Kashif, I.; Soliman, A.; Farouk, H.; El-Shorpagy, M.; Sanad, A. Effect of copper addition on density and magnetic susceptibility of lithium borate glasses. Phys. B: Condens. Matter 2008, 403, 3903–3906. [Google Scholar] [CrossRef]
- Hoppe, A.; Meszaros, R.; Stähli, C.; Romeis, S.; Schmidt, J.; Peukert, W.; Marelli, B.; Nazhat, S.N.; Wondraczek, L.; Lao, J.; et al. In vitro reactivity of Cu doped 45S5 Bioglass® derived scaffolds for bone tissue engineering. J. Mater. Chem. B 2013, 1, 5659–5674. [Google Scholar] [CrossRef] [PubMed]
- Gupta, N.; Santhiya, D.; Murugavel, S.; Kumar, A.; Aditya, A.; Ganguli, M.; Gupta, S. Effects of transition metal ion dopants (Ag, Cu and Fe) on the structural, mechanical and antibacterial properties of bioactive glass. Colloids Surf. A Physicochem. Eng. Asp. 2018, 538, 393–403. [Google Scholar] [CrossRef]
- Hench, L.L.; Wheeler, D.L.; Greenspan, D.C. Molecular Control of Bioactivity in Sol-Gel Glasses. J. Sol-Gel Sci. Technol. 1998, 13, 245–250. [Google Scholar] [CrossRef]
- Stutman, J.M.; Termine, J.D.; Posner, A.S. Vibrational Spectra and Structure of the Phosphate Ion in some Calcium Phosphates. Trans. N.Y. Acad. Sci. 1965, 27, 669–675. [Google Scholar] [CrossRef] [PubMed]
- Rey, C.; Marsan, O.; Combes, C.; Drouet, C.; Grossin, D.; Sarda, S. Characterization of Calcium Phosphates Using Vibrational Spectroscopies. In Advances in Calcium Phosphate Biomaterials; Ben-Nissan, B., Ed.; Springer: Berlin/Heidelberg, Germany, 2014; pp. 229–266. [Google Scholar] [CrossRef]
- Jiménez-Holguín, J.; Sánchez-Salcedo, S.; Vallet-Regí, M.; Salinas, A. Development and evaluation of copper-containing mesoporous bioactive glasses for bone defects therapy. Microporous Mesoporous Mater. 2020, 308, 110454. [Google Scholar] [CrossRef] [PubMed]
- Aina, V.; Cerrato, G.; Martra, G.; Malavasi, G.; Lusvardi, G.; Menabue, L. Towards the controlled release of metal nanoparticles from biomaterials: Physico-chemical, morphological and bioactivity features of Cu-containing sol–gel glasses. Appl. Surf. Sci. 2013, 283, 240–248. [Google Scholar] [CrossRef]
- Zheng, K.; Taccardi, N.; Beltrán, A.M.; Sui, B.; Zhou, T.; Marthala, V.R.R.; Hartmann, M.; Boccaccini, A.R. Timing of calcium nitrate addition affects morphology, dispersity and composition of bioactive glass nanoparticles. RSC Adv. 2016, 6, 95101–95111. [Google Scholar] [CrossRef]
- Gomes, S.; Vichery, C.; Descamps, S.; Martinez, H.; Kaur, A.; Jacobs, A.; Nedelec, J.-M.; Renaudin, G. Cu-doping of calcium phosphate bioceramics: From mechanism to the control of cytotoxicity. Acta Biomater. 2018, 65, 462–474. [Google Scholar] [CrossRef] [PubMed]
- Bazin, T.; Magnaudeix, A.; Mayet, R.; Carles, P.; Julien, I.; Demourgues, A.; Gaudon, M.; Champion, E. Sintering and biocompatibility of copper-doped hydroxyapatite bioceramics. Ceram. Int. 2021, 47, 13644–13654. [Google Scholar] [CrossRef]
Sample | Effective Molar Ratio Ca/Si (EDS) | Effective Composition (wt%) | BET Specific Surface Area (m2/g) | Pore Volume (cm3/g) |
---|---|---|---|---|
MBGN-0.25 | 0.14 ± 0.01 | 88SiO2–12CaO * | 909 ± 14 | 0.65 |
MBGN-0.5 | 0.22 ± 0.01 | 83SiO2–17CaO * | 637 ± 9 | 0.50 |
MBGN-1 | 0.30 ± 0.01 | 78SiO2–22CaO * | 431 ± 4 | 0.34 |
MBGN-2 | 0.37 ± 0.02 | 74SiO2–26CaO * | 208 ± 1 | 0.22 |
Cu-MBGN | 0.25 ± 0.01 | 75.6SiO2–15.5CaO–8.8CuO ** | 418 ± 4 | 0.24 |
Q4 | Q3 | Q2 | |||||
---|---|---|---|---|---|---|---|
Sample | δ (ppm) | pop. (%) | δ (ppm) | pop. (%) | δ (ppm) | pop. (%) | CN |
MBGN-0.5 | –108.74 | 34 | –98.00 | 50 | –84.29 | 16 | 3.16 |
Cu-MBGN | –109.29 | 68 | –98.39 | 32 | –87.72 | 6 | 3.79 |
MBGN-1 | –109.76 | 31 | –98.59 | 50 | –88.01 | 19 | 3.10 |
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Vergnaud, F.; Mekonnen, B.; El Abbassi, A.; Vichery, C.; Nedelec, J.-M. Correlating the Effect of Composition and Textural Properties on Bioactivity for Pristine and Copper-Doped Binary Mesoporous Bioactive Glass Nanoparticles. Materials 2023, 16, 6690. https://doi.org/10.3390/ma16206690
Vergnaud F, Mekonnen B, El Abbassi A, Vichery C, Nedelec J-M. Correlating the Effect of Composition and Textural Properties on Bioactivity for Pristine and Copper-Doped Binary Mesoporous Bioactive Glass Nanoparticles. Materials. 2023; 16(20):6690. https://doi.org/10.3390/ma16206690
Chicago/Turabian StyleVergnaud, Florestan, Benhur Mekonnen, Abdelouahad El Abbassi, Charlotte Vichery, and Jean-Marie Nedelec. 2023. "Correlating the Effect of Composition and Textural Properties on Bioactivity for Pristine and Copper-Doped Binary Mesoporous Bioactive Glass Nanoparticles" Materials 16, no. 20: 6690. https://doi.org/10.3390/ma16206690