In Vitro Assessment of the Antibacterial Potential of Silver Nano-Coatings on Cotton Gauzes for Prevention of Wound Infections
Abstract
:1. Introduction
2. Experimental Section
2.1. Deposition of Silver Nanoparticles on Cotton Gauzes
2.2. SEM Analysis on Silver Coated Gauzes
2.3. Thermo-Gravimetric Analysis TGA
2.4. Qualitative and Quantitative Evaluation of the Antibacterial Capability
2.5. Quantification of Bacterial Biofilm on Cotton Gauzes
2.6. Fluorescence Microscopy on Adherent Bacteria
2.7. SEM Analysis of the Bacterial Adhesion on Cotton Gauzes
2.8. Cytotoxicity Test
2.9. ICP-MS Analysis
3. Results
3.1. Deposition of Silver Nanoparticles on Cotton Gauzes
3.2. SEM Analysis on Silver Coated Gauzes
3.3. Thermo-Gravimetric Analysis TGA
3.4. Qualitative and Quantitative Evaluation of the Antibacterial Capability
3.5. Quantification of Bacterial Biofilm on Cotton Gauzes
3.6. Fluorescence Microscopy on Adherent Bacteria
3.7. SEM Analysis of the Bacterial Adhesion on Cotton Gauzes
3.8. Cytotoxicity Test
3.9. ICP-MS Analysis
4. Discussion
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
Abbreviations
SEM | Scanning Electron Microscopy |
TGA | Thermo-Gravimetric Analysis |
MTT | 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide colorimetric assay |
ICP-MS | Inductively Coupled Plasma Mass Spectrometry |
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Sample | CFU/mL | Log Reduction |
---|---|---|
Untreated | 1.62 × 106 | – |
0.5% Ag | 5.27 × 102 | 3.49 |
4% Ag | 5.00 × 10 | 4.51 |
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Paladini, F.; Di Franco, C.; Panico, A.; Scamarcio, G.; Sannino, A.; Pollini, M. In Vitro Assessment of the Antibacterial Potential of Silver Nano-Coatings on Cotton Gauzes for Prevention of Wound Infections. Materials 2016, 9, 411. https://doi.org/10.3390/ma9060411
Paladini F, Di Franco C, Panico A, Scamarcio G, Sannino A, Pollini M. In Vitro Assessment of the Antibacterial Potential of Silver Nano-Coatings on Cotton Gauzes for Prevention of Wound Infections. Materials. 2016; 9(6):411. https://doi.org/10.3390/ma9060411
Chicago/Turabian StylePaladini, Federica, Cinzia Di Franco, Angelica Panico, Gaetano Scamarcio, Alessandro Sannino, and Mauro Pollini. 2016. "In Vitro Assessment of the Antibacterial Potential of Silver Nano-Coatings on Cotton Gauzes for Prevention of Wound Infections" Materials 9, no. 6: 411. https://doi.org/10.3390/ma9060411