Impact of Soil-Applied Microbial Inoculant and Fertilizer on Fungal and Bacterial Communities in the Rhizosphere of Robinia sp. and Populus sp. Plantations
Round 1
Reviewer 1 Report
Thank you for addressing all my points. In Point 3: What happens to them afterwards, do they persist or are they replaced by others?
I meant what happens to the inoculated mycorrhizal fungi. Whether the inoculated mycorrhizal fungi remained or were replaced by fungal communities that were present in the soil after planting. But thanks for explaining what has or will happen to the plants.
Response 3: In the case of industrial plantations of traditional varieties, the cutting age is 20-25 years. However, the varieties studied in the current experiment are intensively growing varieties, so the time of cutting as well as the age of the plants cannot be predicted. The plants are expected to be grown for shorter time period than traditional varieties.
Author Response
Response to Reviewer 1
Please see the attachment.
Author Response File: 
Author Response.doc
Reviewer 2 Report
General Comments
The article carries out a research on the aspect of Impact of soil-applied microbial inoculant and fertilizer on fungal and bacterial communities in the rhizosphere of Robinia sp. and Populus sp. Plantations.
This topic is very useful and relevant in the scientific field.
Specific Comments for Authors
- Please moderate English changes required
- Please add a map with the study area.
- Please add information regarding the source from which the climatic data were derived.
- In an effort to give readers a better overview of the experiment, please add figures of the experimental stages.
- To help improve the information gaps presented in the article, I leave some key references to improve the quality of the article:
Jeffries, P., Gianinazzi, S., Perotto, S., Turnau, K., & Barea, J. M. (2003). The contribution of arbuscular mycorrhizal fungi in sustainable maintenance of plant health and soil fertility. Biology and fertility of soils, 37(1), 1-16.
Toljander, J. (2006). Interactions between soil bacteria and arbuscular mycorrhizal fungi (Vol. 2006, No. 2006: 39).
Barman, J., Samanta, A., Saha, B., & Datta, S. (2016). Mycorrhiza. Resonance, 21(12), 1093-1104.
Giannoulis, K. D., Skoufogianni, E., Bartzialis, D., Solomou, A. D., & Danalatos, N. G. (2021). Growth and productivity of Salvia officinalis L. under Mediterranean climatic conditions depends on biofertilizer, nitrogen fertilization, and sowing density. Industrial Crops and Products, 160, 113136.
-The conclusions could establish the main limitations of the article. In addition, it could also establish future lines of research that are open or pending after the research is carried out.
Author Response
Response to Reviewer 2
Please see the attachment.
Author Response File: Author Response.doc
Round 2
Reviewer 2 Report
I consider that the review has been carried out following the proposed recommendations.
This manuscript is a resubmission of an earlier submission. The following is a list of the peer review reports and author responses from that submission.
Round 1
Reviewer 1 Report
Dear Authors,
I have reviewed the paper and found it an interesting paper.
General comments
This research deals with important aspects relative to 1) different cultivating mediums during the micro propagation stage to determine which is most suitable to achieve the most favourable growth vigor for maximizing biomass production, 2) the effect of bacterial and fungal inoculation of the symbiosis of black locust (Robinia pseudoacacia L.) and two fertilizer doses (2.5 and 5 kg m-3) on growth in a pot experiment, 3) the soil microbial community changes induced by the microbial inoculation and the different nutrient supplies in intensively cultivated black locust and poplar plantations.
The findings show the below:
Mycorrhizal and bacterial inoculation does interfere with native microbial community to such an extent that it does not significantly affect the rhizosphere bacterial community, which is might influenced by a number of complex environmental factors.
Specific comments
- Please moderate English changes required.
- Line 74-86: Please add the below references
Giannoulis, K. D., Skoufogianni, E., Bartzialis, D., Solomou, A. D., & Danalatos, N. G. (2021). Growth and productivity of Salvia officinalis L. under Mediterranean climatic conditions depends on biofertilizer, nitrogen fertilization, and sowing density. Industrial Crops and Products, 160, 113136.
Dagher, D. J., de la Providencia, I. E., Pitre, F. E., St-Arnaud, M., & Hijri, M. (2020). Arbuscular mycorrhizal fungal assemblages significantly shifted upon bacterial inoculation in non-contaminated and petroleum-contaminated environments. Microorganisms, 8(4), 602.
Diagne, N., Ngom, M., Djighaly, P. I., Fall, D., Hocher, V., & Svistoonoff, S. (2020). Roles of arbuscular mycorrhizal fungi on plant growth and performance: Importance in biotic and abiotic stressed regulation. Diversity, 12(10), 370.
- Cross reference all of the citations in the text with the references in the reference section.
- Make sure that all references have a corresponding citation within the text and vice versa.
- Double check the spelling of the author names and dates and make sure they are correct and consistent with the citations.
- Spell out all journal titles in the reference section.
- Make sure that all figures and tables are cited within the text and they are cited in consecutive order.
- Make sure that all Figure captions are placed below the Figures, while Table captions must be placed above the Tables.
Author Response
Dear Reviewer 1,
Thank you for your comments and advice, we took it into focus when we have improved the manuscript. We worked on revising the manuscript, following the advice of the Assistant Editor and the two reviewers.
We respond to your review in the attachment.
Author Response File: Author Response.doc
Reviewer 2 Report
Dear authors
How old were acacias and poplars when they were inoculated with microorganisms? Was the inoculation of the plants carried out with species characteristic of a certain age of the trees? What happens to them afterwards, do they persist or are they replaced by others? Is it justified to introduce mycorrhizal plants + bacterial inoculation in forest soils where natural myco-organisms colonize the roots?
Despite sterilization of the medium three times, microorganisms other than those inoculated were also found, why? Are the bacteria found always so-called helpers or, on the contrary, are there also those that inhibit the development of mycorrhiza? Such reactions were found in oaks and beeches treated with effective microorganisms, where there were more roots without mycorrhiza than in the control [ https://doi.org/10.3390/f9100597 ].
Did acacias and poplars grow on agricultural soils or on forest soils? Why such a large number of taxa characteristic of agricultural soils? Are they successively replaced by microorganisms characteristic of forest soils? [https://doi.org/10.3390/f10010030].
Throughout the manuscript, check and correct the units of measurement by inserting a space between the number and the unit of measurement, e.g. L153, 154, 174, 176, etc.
When the Latin name is repeated, use the abbreviation of the genus name, e.g., L169 R. pseudoacacia. In L193, the "2" should be superscript. Remove "patents" in L605.
Author Response
Dear Reviewer 2,
Thank you for your comments and advice, we took it into focus when we have improved the manuscript. We worked on revising the manuscript, following the advice of the Assistant Editor and the two reviewers.
We respond to your review in the attachment.
Author Response File: Author Response.doc
