Pathogenicity of Phytophthora× alni Isolates Obtained from Symptomatic Trees, Soil and Water against Alder
Abstract
:1. Introduction
2. Materials and Methods
2.1. Isolates Used in the Study
2.2. Morphologic Characterization of Mycelium, Vegetative and Generative Organs and Effect of Temperature on Mycelium Growth
2.3. Laboratory Tests of Pathogenicity
2.4. Greenhouse Tests of Pathogenicity
2.5. Re-Isolation of the Pathogen
2.6. Statistical Analysis
3. Results
3.1. Morphology of Mycelium, and Vegetative and Generative Organs
3.2. Growth of Mycelium Depending on Medium and Temperature
3.3. Laboratory Tests
3.4. Greenhouse Tests
3.5. Re-Isolation of the Pathogen
3.6. PCA Analysis
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Isolates of P. × alni (Number of Isolates Measured) | Dimensions of Zoosporangia (µm) ± SD | |
---|---|---|
Length | Width | |
P. × alni obtained from alder trunks (18) | 50.4 1 c ± 7.64 | 34.2 c ± 5.1 |
P. × alni obtained from soil (4) | 62.0 a ± 6.47 | 45.0 a ± 4.1 |
P. × alni obtained from water (7) | 54.5 b ± 6.23 | 36.6 b ± 4.1 |
Isolates of P. × alni (Number of Isolates Evaluated) | Daily Growth of Hyphae at 25 °C (mm/24 h) | ||
---|---|---|---|
PDA | V8 | CA | |
P. × alni obtained from alder trunks (18) | 3.82 1 c ± 0.19 | 6.81 b ± 0.23 | 9.48 a ± 0.14 |
P. × alni obtained from soil (4) | 3.86 c ± 0.20 | 9.11 a ± 0.26 | 7.11 b ± 0.26 |
P. × alni obtained from water (7) | 3.78 c ± 0.21 | 9.14 a ± 0.32 | 7.11 b ± 0.33 |
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Trzewik, A.; Maciorowski, R.; Orlikowska, T. Pathogenicity of Phytophthora× alni Isolates Obtained from Symptomatic Trees, Soil and Water against Alder. Forests 2022, 13, 20. https://doi.org/10.3390/f13010020
Trzewik A, Maciorowski R, Orlikowska T. Pathogenicity of Phytophthora× alni Isolates Obtained from Symptomatic Trees, Soil and Water against Alder. Forests. 2022; 13(1):20. https://doi.org/10.3390/f13010020
Chicago/Turabian StyleTrzewik, Aleksandra, Robert Maciorowski, and Teresa Orlikowska. 2022. "Pathogenicity of Phytophthora× alni Isolates Obtained from Symptomatic Trees, Soil and Water against Alder" Forests 13, no. 1: 20. https://doi.org/10.3390/f13010020
APA StyleTrzewik, A., Maciorowski, R., & Orlikowska, T. (2022). Pathogenicity of Phytophthora× alni Isolates Obtained from Symptomatic Trees, Soil and Water against Alder. Forests, 13(1), 20. https://doi.org/10.3390/f13010020