Comprehensive Bioinformatics and Expression Analysis of TCP Transcription Factors in Liriodendron chinense Reveals Putative Abiotic Stress Regulatory Roles
, Yuanlin Guan 1, Rongxue Li 1, Ali Movahedi 1, Jinhui Chen 2,* and Liming Yang 1,*
Round 1
Reviewer 1 Report
The paper “Comprehensive Bioinformatics and Expression Analysis of TCP Transcription Factors in Liriodendron chinense reveals putative abiotic stress regulatory roles” written by Hwarari et al., has in silico identified The TEOSINTE BRANCHED 1/CYCLOI- 13 DEA/PROLIFERATING CELL FACTORS (TCP) proteins from Liriodendron chinense. As per earlier reports this transcription factor is known for their growth and developmental roles in various plant species. The current study has indicated the potential use of this transcription factor in controlling various abiotic stress conditions in this economically, and ecologically important plant. I have following points for the change in manuscript…
1. Many scientific names are not italic. Please revise the manuscript for typo errors.
2. Liu, H., Ma, J. & Li, H. Transcriptomic and microstructural analyses in Liriodendron tulipifera Linn. reveal candidate genes involved in nectary development and nectar secretion. BMC Plant Biol 19, 531 (2019). https://doi.org/10.1186/s12870-019-2140-0
This study has already identified TCP gene and their gene expression in Liriodendron tulipifera. Why this study is not cited or used for bioinformatic analysis?
3. Line 59: Please revise and remove “Arabidopsis” as two crops are mentioned in example.
4. Line 75: FT-FD full form is not given
5. Line 84-87: “An assortment of AtTCPs class I (AtTCP6/20/23) were shown upregulate the early onset of the plant Mediator, AtTCP7/9/16/21 were also demonstrated to upregulate the late response the plant mediator, and AtTCP2/21/13/17/3/5 were shown to upregulate the early-down and late response of the plant mediator.” Is not clear and need revision.
6. Line 107-111: Remove the redundancy of mentioning the plant species.
7. Was the two different experiment was set up for preparing the plant material for transcriptome sequencing and qRT-PCR expression analysis? If yes, mention it clearly with the logic behind doing this. If no, then revise the material section for clarity.
8. How the drought treatment was given is not mentioned.
9. As the authors have transcriptome data of stressed plant tissue, they can analyze the expression pattern of downstream genes of TCP.
10. Line 680-681: Remove “This section is not mandatory but can be added to the manuscript if the discussion is 680 unusually long or complex.”
Author Response
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Author Response File: 
Author Response.pdf
Reviewer 2 Report
In the submitted article " Comprehensive Bioinformatics and Expression Analysis of TCP Transcription Factors in Liriodendron chinense reveals putative abiotic stress regulatory roles", the authors were able to identify 15 LcTCPs in the genome of L. chinense comprising at least LcTCP1 as a potential regulator of abiotic stress in the L. chinense genome. Interestingly, they addressed much more of LcTCPs characteristics using a punch of bioinformatics tools including gene structure, location, phylogenetic relationships, evolution divergences, promoter elements, and expression patterns to three abiotic stresses. I think this study has brought a successful endeavor and was accomplished in more brilliant practical form, contributing to the functional analyses of LcTCP genes in the L. chinense genome.
I recommend comprehensive English polishing for whole the manuscript. Also, some minor typos need to be corrected as follows:
Line 15: pls take care for the italic format of scientific name of “L. chinense” everywhere in the manuscript.
Line 80: jasmonic not Jasmonic
Line 84-88: pls clarify this sentence.
Line 84-88: pls clarify this sentence.
Line 108: 3 Eudicots, shouldn’t start the sentence with a number, it should be written in letters.
Line 112: (E-value1.0E-10) is not written in the correct form.
Line 138: please correct the sentence “t 0_h, 6_h, 1_d, and 3_d “, it’s not written following the correct form…it should like what is written in line 151.
Line 168: 15LcTCPs ?
Line 185: 1e-5 ?
Line 204: Using Mega 11, …….
Line 250: species TCP, members we ???
Line 257: accordingly???
Figure 11: Please indicate the significance among different expression patterns using letters a, b, c “letters of significance”.
Author Response
Please see attachment
Author Response File: Author Response.pdf
