Proteomics Analysis and Identification of Proteins Related to Isoprenoid Biosynthesis in Cinnamomum camphora (L.) Presl

Round 1

Reviewer 1 Report

In the article of “Proteomics analysis and identification of proteins related to isoprenoid biosynthesis in Cinnamomum camphora”, authors found that the content of linalool was higher in 65-day-old leaves than that in 15-day-old leaves of linalool-type C. camphora, and many candidate proteins which participated in isoprenoid biosynthesis through proteomics analysis , Additionally, the potential transcription factor families which may play key roles in the leaf development of linalool-type C. camphora. The interesting research will provide valuable information for further investigation of isoprenoid metabolic in C. camphora. However, it needs to be further improved before it could be published.

1. In the Introduction, it would be better to add a figure for isoprenoids metabolic pathway,like lines 48-73.

2. In the Materials and methods, methods references were all missing.

3. In the Results

Lines 187, why choose the 15th and 65th as the sample time, and it is not enough for analysing the linalool content of C. camphora leaves at different stages just through two time points.

Lines 216, please give the appropriate explains for this figure in the text content, i think it should be put in the supplement files.

Lines 242, it is some problems in the Figure 3, please correct.

Lines 262, what’s the means of “Both up-regulated and down-regulated in the 65d”. 

Lines 285, please give the reason of the relationship between transcription factors and the isoprenoid biosynthesis.

4. In the Discussion,

lines 303, through proteomic analysis of C. camphora leaf, it is far from enough for e elucidating the molecular mechanisms underlying the regulation of isoprenoid synthesis at different development stages in C. camphora. Fristly, more time points for isoprenoid synthesis in C. camphora should be added. Secondly, proteomic analysis results should be verified for ensuring data reliability. Thirdly, another analyse should be performed, for example, transcript levels analysis, metabolism analyse, etc.

5. In the Conclusions

   Lines 342, the role of ‘transcription factor families ’ in the leaf development of linalool-type  C. camphora needed given more related analysis and experiments.

Author Response

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Author Response File: Author Response.docx

Reviewer 2 Report

This is an interesting project and the manuscript was reasonably well written. However, there are some key features missing in the manuscript: (1) The authors need to indicate whether sample replications have been done in their experimental design. (2) Discussion and conclusion need much improvement where key findings need to be elaborated. 

Comments for author File: Comments.pdf

Author Response

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Author Response File: Author Response.docx