Morphological, Histological, and Transcriptome Analysis of Doubled Haploid Plants in Poplars (Populus simonii × Populus nigra)
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Materials
2.2. Morphological and Histological Analysis
2.3. Sample Preparation, cDNA Library Construction and Illumina Sequencing
2.4. Differential Expression Profile
2.5. Hormone Treatment
2.6. qRT-PCR Analysis
3. Results
3.1. Phenotypic Characteristics of the DH Plants
3.2. Identification of Differentially Expressed Genes between the DH Plants and the Diploid Plants
3.3. GO Enrichment and KEGG Pathway Analysis of the DEGs
3.4. Expression Analysis of Hormone-Responsive Genes in WT
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Wang, Y.; Yu, J.; Zhang, X.; He, Y.; Chen, S.; Fan, E.; Qu, G.; Chen, S.; Liu, C. Morphological, Histological, and Transcriptome Analysis of Doubled Haploid Plants in Poplars (Populus simonii × Populus nigra). Forests 2023, 14, 1535. https://doi.org/10.3390/f14081535
Wang Y, Yu J, Zhang X, He Y, Chen S, Fan E, Qu G, Chen S, Liu C. Morphological, Histological, and Transcriptome Analysis of Doubled Haploid Plants in Poplars (Populus simonii × Populus nigra). Forests. 2023; 14(8):1535. https://doi.org/10.3390/f14081535
Chicago/Turabian StyleWang, Yiran, Jiajie Yu, Xiang Zhang, Yaxin He, Song Chen, Erqin Fan, Guanzheng Qu, Su Chen, and Caixia Liu. 2023. "Morphological, Histological, and Transcriptome Analysis of Doubled Haploid Plants in Poplars (Populus simonii × Populus nigra)" Forests 14, no. 8: 1535. https://doi.org/10.3390/f14081535