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Open AccessArticle
Function of the NAC1 Gene from Fraxinus mandshurica in Cold Resistance and Growth Promotion in Tobacco
by
Yang Cao
Yang Cao 1,2,3,†,
Liming He
Liming He 1,2,3,†,
Shengdian Lu
Shengdian Lu 1,2,3,
Yuling Wang
Yuling Wang 1,2,3,
Chenxi Zhang
Chenxi Zhang 1,2,3 and
Yaguang Zhan
Yaguang Zhan 1,2,3,*
1
National Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, China
2
College of Life Sciences, Northeast Forestry University, Harbin 150040, China
3
Heilongjiang Touyan Innovation Team Program (Tree Genetics and Breeding Innovation), Northeast Forestry University, Harbin 150040, China
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Forests 2024, 15(8), 1405; https://doi.org/10.3390/f15081405 (registering DOI)
Submission received: 20 May 2024
/
Revised: 7 August 2024
/
Accepted: 9 August 2024
/
Published: 10 August 2024
Abstract
To elucidate the function of the cold-resistance regulatory gene FmNAC1 from Fraxinus mandshurica Rupr., this study identified the role that overexpression of the FmNAC1 gene plays in tobacco growth and cold-stress regulation. The cloned FmNAC1 gene from F. mandshurica is 891 bp in length and encodes 296 amino acids. Our subcellular localization analysis confirmed that FmNAC1 is primarily located in the nucleus and functions as a transcription factor. FmNAC1 is responsive to cold and NaCl stress, as well as to the induction of IAA, GA, and ABA hormone signals. To further elucidate its function in cold resistance, four transgenic tobacco lines expressing FmNAC1 (FmNAC1-OE) were generated through tissue culture after the Agrobacterium-mediated transformation of wild-type (WT) Nicotiana tabacum L.. These FmNAC1-OE plants exhibited accelerated growth after transplantation. When exposed to low-temperature conditions at −5 °C for 24 h, the rates of wilting and yellowing of the FmNAC1-OE plants were significantly lower than those of the WT tobacco plants. Additionally, the membrane integrity, osmotic regulation, and reactive oxygen species (ROS)-scavenging abilities of the FmNAC1-OE tobacco lines were better than those of the WT plants, indicating the potential of the FmNAC1 gene to improve plant cold resistance. The gene expression results further revealed that the FmNAC1 transcription factor exhibits regulatory interactions with growth-related genes such as IAA and AUX1; cold-resistance-related genes such as ICE, DREB, and CBF1; and genes involved in the clearance of reactive oxygen species (ROS), such as CAT and SOD. All of this evidence shows that the FmNAC1 transcription factor from F. mandshurica plays a key role in contributing to the enhancement of growth, cold resistance, and ROS clearance in transgenic tobacco plants.
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MDPI and ACS Style
Cao, Y.; He, L.; Lu, S.; Wang, Y.; Zhang, C.; Zhan, Y.
Function of the NAC1 Gene from Fraxinus mandshurica in Cold Resistance and Growth Promotion in Tobacco. Forests 2024, 15, 1405.
https://doi.org/10.3390/f15081405
AMA Style
Cao Y, He L, Lu S, Wang Y, Zhang C, Zhan Y.
Function of the NAC1 Gene from Fraxinus mandshurica in Cold Resistance and Growth Promotion in Tobacco. Forests. 2024; 15(8):1405.
https://doi.org/10.3390/f15081405
Chicago/Turabian Style
Cao, Yang, Liming He, Shengdian Lu, Yuling Wang, Chenxi Zhang, and Yaguang Zhan.
2024. "Function of the NAC1 Gene from Fraxinus mandshurica in Cold Resistance and Growth Promotion in Tobacco" Forests 15, no. 8: 1405.
https://doi.org/10.3390/f15081405
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