Increased Soil Moisture in the Wet Season Alleviates the Negative Effects of Nitrogen Deposition on Soil Microbial Communities in Subtropical Evergreen Broad-Leaved Forest
Abstract
:1. Introduction
2. Materials and Methods
2.1. Study Area
2.2. Experimental Design
2.3. Sample Collection and Processing
2.4. Measurement of Indicators
2.5. Data Analysis
3. Results
3.1. Soil Chemical Properties under N Deposition
3.2. Soil Enzyme Activities under N Deposition
3.3. Soil Microbial Community Structure under N Deposition
3.3.1. Soil Microbial α-Diversity
3.3.2. Soil Microbial β-Diversity
3.4. Soil Microbial Community Composition
3.5. Correlation between Microbial Community Structure and Soil Environmental Factors
4. Discussion
4.1. Effects of N Deposition on Soil Chemical Properties and Enzyme Activities
4.2. Effects of N Deposition on the Structure and Composition of Soil Microbial Communities
4.3. Correlation of Soil Microbial Communities with Environmental Factors
5. Conclusions
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Stand | Altitude/m | Age/a | H/m | DBH/cm | Canopy Density | Slope/(o) | Aspect | Soil Category |
---|---|---|---|---|---|---|---|---|
1 | 2130 | 17 | 9.6 | 14.5 | 0.87 | 23 | NE | Argi-udic Ferrosols |
2 | 2132 | 15 | 12.1 | 20.7 | 0.90 | 28 | NE | Argi-udic Ferrosols |
3 | 2133 | 17 | 10.8 | 18.3 | 0.85 | 30 | NE | Argi-udic Ferrosols |
Abbreviation | Full Name | Unit | Abbreviation | Full Name |
---|---|---|---|---|
CK | Control | 0 g·N·m−2·a−1 | OTUs | Operational taxonomic units |
LN | Low nitrogen | 10 g·N·m−2·a−1 | Chao | Chao 1 index |
MN | Medium nitrogen | 20 g·N·m−2·a−1 | Shannon | Shannon index |
HN | High nitrogen | 25 g·N·m−2·a−1 | BOTUs | Number of bacterial OUT items |
pH | Acidity and alkalinity | FOTUs | Number of fungal OUT items | |
SOC | Soil organic carbon | g·kg−1 | Bchao | Bacterial chao1 index |
NO3−-N | Nitrate nitrogen | mg·kg−1 | Fchao | Fungal chao1 index |
NH4+-N | Ammonium nitrogen | mg·kg−1 | Bshannon | Bacterial Shannon index |
TN | Total nitrogen | g·kg−1 | Fshannon | Fungal Shannon index |
TK | Total potassium | g·kg−1 | bpc1 | Bacterial community |
AP | Available phosphorus | mg·kg−1 | bpc2 | Bacterial community |
TP | Total phosphorus | g·kg−1 | fpc1 | Fungi community |
CAT | Catalase | U/g | fpc2 | Fungi community |
SC | Sucrase | U/g | EBLFs | Evergreen broad-leaved forests |
UE | Urease | U/g | SMCs | Soil microbial communities |
ACP | Acid phosphatase | U/g |
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Chen, W.; Hou, Z.; Zhang, D.; Chen, L.; Wang, K.; Song, Y. Increased Soil Moisture in the Wet Season Alleviates the Negative Effects of Nitrogen Deposition on Soil Microbial Communities in Subtropical Evergreen Broad-Leaved Forest. Forests 2024, 15, 1473. https://doi.org/10.3390/f15081473
Chen W, Hou Z, Zhang D, Chen L, Wang K, Song Y. Increased Soil Moisture in the Wet Season Alleviates the Negative Effects of Nitrogen Deposition on Soil Microbial Communities in Subtropical Evergreen Broad-Leaved Forest. Forests. 2024; 15(8):1473. https://doi.org/10.3390/f15081473
Chicago/Turabian StyleChen, Wen, Zheng Hou, Donghui Zhang, Leixi Chen, Keqin Wang, and Yali Song. 2024. "Increased Soil Moisture in the Wet Season Alleviates the Negative Effects of Nitrogen Deposition on Soil Microbial Communities in Subtropical Evergreen Broad-Leaved Forest" Forests 15, no. 8: 1473. https://doi.org/10.3390/f15081473