Transcriptomic Profiling Reveals That the Differentially Expressed PtNAC9 Transcription Factor Stimulates the Salicylic Acid Pathway to Enhance the Defense Response against Bursaphelenchus xylophilus in Pines
Abstract
:1. Introduction
2. Results
2.1. Identification of Differentially Expressed Transcription Factors (TFs)
2.2. Functional Enrichment and Annotation of TFs in Response to B. xylophilus
2.3. Validation of TFs by Real Time-Quantitative PCR
2.4. Subcellular Localization and Functional Verification of PtNAC9
2.5. PtNAC9 Positively Regulates the Resistance of Pines to B. xylophilus
2.6. PtNAC9 Influences the Accumulation of Defense-Related Plant Hormones and Genes
3. Discussion
4. Materials and Methods
4.1. Identification of Differentially Expressed Transcription Factors (DETFs)
4.2. Functional Annotation and Pathway Analysis
4.3. Real-Time Quantitative PCR
4.4. Agrobacterium Tumefaciens Infiltration Assays
4.5. Subcellular Localization
4.6. Eukaryotic Expression of the Recombinant PtNAC9 Protein
4.7. Inoculation Assay
4.8. Determination of Salicylic Acid and Enzyme Activity in Pines
4.9. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Wen, T.-Y.; Wang, X.-Y.; Wu, X.-Q.; Ye, J.-R. Transcriptomic Profiling Reveals That the Differentially Expressed PtNAC9 Transcription Factor Stimulates the Salicylic Acid Pathway to Enhance the Defense Response against Bursaphelenchus xylophilus in Pines. Forests 2024, 15, 1538. https://doi.org/10.3390/f15091538
Wen T-Y, Wang X-Y, Wu X-Q, Ye J-R. Transcriptomic Profiling Reveals That the Differentially Expressed PtNAC9 Transcription Factor Stimulates the Salicylic Acid Pathway to Enhance the Defense Response against Bursaphelenchus xylophilus in Pines. Forests. 2024; 15(9):1538. https://doi.org/10.3390/f15091538
Chicago/Turabian StyleWen, Tong-Yue, Xin-Yu Wang, Xiao-Qin Wu, and Jian-Ren Ye. 2024. "Transcriptomic Profiling Reveals That the Differentially Expressed PtNAC9 Transcription Factor Stimulates the Salicylic Acid Pathway to Enhance the Defense Response against Bursaphelenchus xylophilus in Pines" Forests 15, no. 9: 1538. https://doi.org/10.3390/f15091538