Tolerance of Triploid Hybrids of White Poplar ‘Beilinxiongzhu 1’ to Genetic Transformation Screening Agents In Vitro
Abstract
:1. Introduction
2. Materials and Methods
2.1. Test Materials
2.2. Test Method
2.2.1. Determination of Hyg, Kan, and PPT Sensitivities
2.2.2. Transcriptome Sequencing
2.2.3. WGCNA Analysis
2.2.4. qRT-PCR Validation
3. Results
3.1. Determination of the Screening Concentrations of the Three Antibiotics
3.2. Physiological Response of Poplar Leaves to Antibiotics
3.3. Transcriptome Analysis of the Antibiotic-Treated Leaves
3.3.1. Quality Control of the Transcriptome Data
3.3.2. Intra- and Inter-Group Differentially Expressed Genes
3.3.3. GO Functional Enrichment Analysis of the DEGs in Group Comparisons
3.3.4. KEGG Functional Enrichment Analysis of the DEGs in Group Comparisons
3.3.5. Analysis of the DEGS across the Treatment Groups
Inter-Group Specific DEGS Analysis
3.4. Gene Co-Expression Network Analysis
Analysis of Central Genes Related to Physiological Indexes
3.5. qRT-PCR Validation Results
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
Abbreviations
Hyg | hygromycin |
Kan | kanamycin |
PPT | glyphosate |
DEGs | differentially expressed genes |
MS | Murashige and Skoog |
6BA | 6-benzylaminopurine |
IBA | Indolebutyric acid |
NAA | naphthalene acetic acid |
IAA | Indole-3-Acetic Acid |
ZT | Zeatin |
SS | Soluble sugars |
SP | soluble proteins |
GO | gene ontology |
KEGG | Kyoto Encyclopedia of Genes and Genomes |
BP | biological process |
CC | cellular component |
MF | molecular function |
qRT-PCR | real-time fluorescent quantitative PCR |
WGCNA | weighted gene co-expression network analysis |
GSH | glutathione |
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Liu, L.; Zhang, J.; Song, Y.; Xu, Y.; Wang, S.; Yang, G.; Yang, M. Tolerance of Triploid Hybrids of White Poplar ‘Beilinxiongzhu 1’ to Genetic Transformation Screening Agents In Vitro. Forests 2024, 15, 1580. https://doi.org/10.3390/f15091580
Liu L, Zhang J, Song Y, Xu Y, Wang S, Yang G, Yang M. Tolerance of Triploid Hybrids of White Poplar ‘Beilinxiongzhu 1’ to Genetic Transformation Screening Agents In Vitro. Forests. 2024; 15(9):1580. https://doi.org/10.3390/f15091580
Chicago/Turabian StyleLiu, Lingyun, Jun Zhang, Yuying Song, Ying Xu, Shijie Wang, Gaixia Yang, and Minsheng Yang. 2024. "Tolerance of Triploid Hybrids of White Poplar ‘Beilinxiongzhu 1’ to Genetic Transformation Screening Agents In Vitro" Forests 15, no. 9: 1580. https://doi.org/10.3390/f15091580