Integrated Transcriptome and Metabolome Analyses Provided Insight into the Variation in Quality of Green Bamboo (Bambusa oldhamii) Shoots at Different Growth Stages
Abstract
:1. Introduction
2. Materials and Methods
2.1. Study Site and Plant Materials
2.2. Determination of Lignin, Cellulose, Hemicellulose and Total Flavonoid Content
2.3. Metabolite Extraction and Analysis
2.4. RNA Extraction, Library Construction, and Transcriptome Analysis
2.5. Quantitative Real-Time-PCR Validation
2.6. Statistical Analysis
3. Results
3.1. Changes of Lignin, Cellulose, Hemicellulose and Flavonoid Contents of Green Bamboo Shoots at Three Growth Stages
3.2. Metabolomic Changes at Three Growth Stages
3.3. Transcriptome Analysis and Identification of DEGs of Three Growth Stages
3.4. DEGs and DAMs Related to Lignin and Flavonoid Synthesis
3.5. Validation of Gene Expression in Green Bamboo Shoots via qRT-PCR
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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He, Y.; Cao, X.; Pan, K.; Shi, W.; Ying, Y. Integrated Transcriptome and Metabolome Analyses Provided Insight into the Variation in Quality of Green Bamboo (Bambusa oldhamii) Shoots at Different Growth Stages. Forests 2024, 15, 1582. https://doi.org/10.3390/f15091582
He Y, Cao X, Pan K, Shi W, Ying Y. Integrated Transcriptome and Metabolome Analyses Provided Insight into the Variation in Quality of Green Bamboo (Bambusa oldhamii) Shoots at Different Growth Stages. Forests. 2024; 15(9):1582. https://doi.org/10.3390/f15091582
Chicago/Turabian StyleHe, Yuelin, Xiangmei Cao, Kaiting Pan, Wenhui Shi, and Yeqing Ying. 2024. "Integrated Transcriptome and Metabolome Analyses Provided Insight into the Variation in Quality of Green Bamboo (Bambusa oldhamii) Shoots at Different Growth Stages" Forests 15, no. 9: 1582. https://doi.org/10.3390/f15091582