Development of 5S rDNA-Based Molecular Markers for the Identification of Populus deltoides Bartr. ex Marshall, Populus nigra L., and Their Hybrids
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Material and DNA Isolation
2.2. Analysis of Sequences and Primer Design
2.3. PCR and Electrophoresis
3. Results
4. Discussion
Supplementary Materials
Author Contributions
Acknowledgments
Conflicts of Interest
References
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Species | Parents of Hybrid | Sample Name | Co-Ordinates |
---|---|---|---|
P. nigra L. | tree#3 | 55°83′51.59′′ 37°55′45.48′′ | |
tree#10a | 55°81′94.18′′ 37°56′81.44′′ | ||
tree#15 | 55°76′77.41′′ 37°56′47.46′′ | ||
tree#16 | 55°76′78.05′′ 37°56′47.74′′ | ||
tree#10b | 50°83′42.96′′ 39°39′21.76′′ | ||
P. deltoides Bartr. ex Marshall | tree#4 | 55°83′51.72′′ 37°55′55.98′′ | |
tree#1 | 55°81′89.74′′ 37°56′83.83′′ | ||
tree#13 | 55°83′3.25′′ 37°57′52.70′′ | ||
P. × canadensis Moench. | P. nigra L. × P. deltoides Bartr. ex Marshall | tree#11 | 55°82′83.67′′ 37°57′47.36′′ |
tree#14 | 55°83′5.53′′ 37°57′47.93′′ | ||
tree#2 | 55°81′63.37′′ 37°56′94.05′′ | ||
tree#17 | 55°77′0.49′′ 37°56′56.19′′ | ||
P. trichocarpa Torr. et A. Gray | tree#20 | 55°83′48.51′′ 37°55′55.97′′ | |
P. maximowiczii Henry | tree#21 | 55°83′52.52′′ 37°55′53.57′′ | |
P. simonii Can. | tree#22 | 55°83′52.74′′ 37°55′54.93′′ | |
P. candicans Ait. | tree#23 | 55°83′53.31′′ 37°55′52.29′′ | |
P. alba L. | tree#24 | 55°81′46.58′′ 37°55′98.41′′ | |
P. tremula L. | tree#25 | 55°83′50.41′′ 37°55′53.78′′ | |
P. × moskoviensis R. I. Schrod. | P. suaveolens Fish. × P. laurifolia Ldb. | tree#26 | 55°83′53.36′′ 37°55′54.88′′ |
P. × sowietica pyramidalis Jabl. | P. alba L. × P. bolleana Lauche | tree#27 | 55°83′67.63′′ 37°55′52.73′′ |
P. × jablokovii Jabl. | P. tremula L. × P. bolleana Lauche | tree#28 | 55°97′77.66′′ |
37°89′60.74′′ | |||
P. × berolinensis K. Koch. | P. laurifolia Ldb. × P. nigra L. | tree#29 | 55°83′51.80′′ 37°55′55.15′′ |
Primer Name | Sequence | Annealing, t °C 1 | PCR Product Length, bp |
---|---|---|---|
5S1mod1 | 5′-GGATGGGTGACCTCCCG-3′ | 62.8 | 485 |
NIG6-2r | 5′-TTCGAAGGAGCCGCGC-3′ | ||
5S1mod2 | 5′-GGATGGGTGACCTCCGG-3′ | 66.0 | 433 |
DEL121-4r | 5′-GGCGACCCTGGGAAGTGT-3′ |
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Alexandrov, O.S.; Karlov, G.I. Development of 5S rDNA-Based Molecular Markers for the Identification of Populus deltoides Bartr. ex Marshall, Populus nigra L., and Their Hybrids. Forests 2018, 9, 604. https://doi.org/10.3390/f9100604
Alexandrov OS, Karlov GI. Development of 5S rDNA-Based Molecular Markers for the Identification of Populus deltoides Bartr. ex Marshall, Populus nigra L., and Their Hybrids. Forests. 2018; 9(10):604. https://doi.org/10.3390/f9100604
Chicago/Turabian StyleAlexandrov, Oleg S., and Gennady I. Karlov. 2018. "Development of 5S rDNA-Based Molecular Markers for the Identification of Populus deltoides Bartr. ex Marshall, Populus nigra L., and Their Hybrids" Forests 9, no. 10: 604. https://doi.org/10.3390/f9100604