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10.3390/v13010025
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Article

Molecular Characteristics of Jujube Yellow Mottle-Associated Virus Infecting Jujube (Ziziphus jujuba Mill.) Grown at Aksu in Xinjiang of China

by
Jiashu Guo
1,2,†,
Yanxiang Wang
1,2,†,
Guoping Wang
1,2,
Jian Hong
3,
Zuokun Yang
1,2,
Jianyu Bai
4,* and
Ni Hong
1,2,*
1
Key Lab of Plant Pathology of Hubei Province, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
2
Key Laboratory of Horticultural Crop (Fruit Trees) Biology and Germplasm Creation of the Ministry of Agriculture, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, China
3
Analysis Center of Agrobiology and Environmental Sciences, Zhejiang University, Hangzhou 317502, China
4
Laboratory of Fruit Trees Disease, Institute of Economic Forestry, Xinjiang Academy of Forestry Sciences, Urumqi 830063, China
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Viruses 2021, 13(1), 25; https://doi.org/10.3390/v13010025
Submission received: 29 November 2020 / Revised: 22 December 2020 / Accepted: 23 December 2020 / Published: 25 December 2020
(This article belongs to the Special Issue Genomics in Plant Viral Research)
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Abstract

Chinese jujube (Ziziphus jujuba Mill.) is a native fruit crop in China. Leaf mottle and dapple fruit disease is prevalent in cultivated jujube plants grown at Aksu in Xinjiang Uygur Autonomous Region of China. Jujube yellow mottle-associated virus (JYMaV), a tentative member in the genus Emaravirus, was recently identified from mottle-diseased jujube plants grown in Liaoning Province in China, but its incidence and genetic diversity in China is unknown. In this study, the genome sequences of three JYMaV isolates from two jujube cultivars and one jujube variant were determined by high-throughput sequencing (HTS) for small RNA and rRNA-depleted RNA coupled with RT-PCR assays. Comparison of these sequences together with sequences of the viral RNA segments derived by primer set 3C/5H-based RT-PCR revealed that genetic diversity was present in the virus populations and high sequence variation occurred at the non-translational regions of each of the viral genomic segments. Field investigation confirmed the close association of the virus with leaf mottle symptoms of jujube plants. Furthermore, this study revealed that P5 encoded in the viral RNA5 displayed a nuclear localization feature differing from the plasmodesma (PD) subcellular localization of the virus movement protein (P4), and the two proteins could interact with each other in the BiFC assays. Our study provides a snapshot of JYMaV genetic diversity in its natural hosts.
Keywords: jujube; high-throughput sequencing; Emaravirus; genetic diversity jujube; high-throughput sequencing; Emaravirus; genetic diversity

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MDPI and ACS Style

Guo, J.; Wang, Y.; Wang, G.; Hong, J.; Yang, Z.; Bai, J.; Hong, N. Molecular Characteristics of Jujube Yellow Mottle-Associated Virus Infecting Jujube (Ziziphus jujuba Mill.) Grown at Aksu in Xinjiang of China. Viruses 2021, 13, 25. https://doi.org/10.3390/v13010025

AMA Style

Guo J, Wang Y, Wang G, Hong J, Yang Z, Bai J, Hong N. Molecular Characteristics of Jujube Yellow Mottle-Associated Virus Infecting Jujube (Ziziphus jujuba Mill.) Grown at Aksu in Xinjiang of China. Viruses. 2021; 13(1):25. https://doi.org/10.3390/v13010025

Chicago/Turabian Style

Guo, Jiashu, Yanxiang Wang, Guoping Wang, Jian Hong, Zuokun Yang, Jianyu Bai, and Ni Hong. 2021. "Molecular Characteristics of Jujube Yellow Mottle-Associated Virus Infecting Jujube (Ziziphus jujuba Mill.) Grown at Aksu in Xinjiang of China" Viruses 13, no. 1: 25. https://doi.org/10.3390/v13010025

APA Style

Guo, J., Wang, Y., Wang, G., Hong, J., Yang, Z., Bai, J., & Hong, N. (2021). Molecular Characteristics of Jujube Yellow Mottle-Associated Virus Infecting Jujube (Ziziphus jujuba Mill.) Grown at Aksu in Xinjiang of China. Viruses, 13(1), 25. https://doi.org/10.3390/v13010025

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