Human Papillomavirus Detected in Oropharyngeal Cancers from Chilean Subjects
Abstract
:1. Introduction
2. Materials and Methods
2.1. Sample Collection
2.2. DNA Extraction, HPV Detection and Genotyping
2.3. Tissue Microarray (TMA) Construction and p16 Immunohistochemistry
2.4. RNA Purification and Transcriptase-Reverse PCR
2.5. Statistical Analysis
3. Results
3.1. Clinical Pathological Characteristics
3.2. HPV Detection and Genotyping
3.3. p16 Immunohistochemistry
3.4. E6/E7 Transcripts in OPSCCs
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Anatomical Site | |||||
---|---|---|---|---|---|
Features | Palatine Tonsils N (%) | Tongue Base N (%) | Soft Palate N (%) | Total | p-Value |
Gender | p = 0.85 | ||||
Female | 9 (50) | 6 (33.33) | 3 (16.66) | 18 | |
Male | 17 (54.38) | 8 (25.8) | 6 (19.35) | 31 | |
Age | p = 0.3035 | ||||
≤65 years | 18 (62.07) | 7 (24.24) | 4 (13.79) | 29 | |
>65 years | 8 (40) | 7 (35) | 5 (25) | 20 | |
Keratinization | p = 0.2115 | ||||
Keratinized | 15 (45.45) | 10 (30.3) | 8 (24.24) | 33 | |
Non-Keratinized | 11 (68.75) | 4 (25) | 1 (6.25) | 16 | |
Differentation | p = 0.0323 * | ||||
Poor | 17 (69.56) | 5 (21.73) | 2 (8.26) | 23 | |
Moderate | 8 (47%) | 4 (23.52) | 5 (29.41) | 17 | |
Well | 1 (12.5) | 5 (62.5) | 2 (25) | 8 | |
p16 IHC | p < 0.0001 *** | ||||
Positive | 22 (78.57) | 5 (17.85) | 1 (3.57) | 28 | |
Negative | 3 (15) | 9 (45) | 8 (40) | 20 |
Variable | Number of Cases (%) | |
---|---|---|
HPV Presence | HPV-Negative | 19 (38.8) |
HPV-Positive | 30 (61.2) | |
Oncogenic Risk | Low | 3 (10) |
High | 27 (90) | |
Genotype | HPV6 | 3 (10) |
HPV16 | 24 (80) | |
HVP18 | 1 (3.3) | |
HVP33 | 2 (6.7) |
Feature | HPV Presence | |||
---|---|---|---|---|
HPV-Negative | HPV-Positive | Total | p-Value | |
Gender | p = 0.24 | |||
Female | 9 (50) | 9 (50) | 18 | |
Male | 10 (32.25) | 21 (67.74) | 31 | |
Age | p = 0.3759 | |||
≤65 years | 9 (32.14) | 19 (67.85) | 28 | |
>65 years | 9 (45) | 11 (55) | 20 | |
Smoking Status | p = 0.95 | |||
Never | 4 (33,33) | 8 (66,77) | 12 | |
Former | 2 (40) | 3 (60) | 5 | |
Currently | 5 (33,33) | 10 (66,77) | 15 | |
Keratinization | p = 0.11 | |||
Keratinized | 16 (47.06) | 18 (52.94) | 34 | |
Non-keratinized | 3 (20) | 12 (80) | 15 | |
Differentiation | p = 0.029 * | |||
Poor | 7 (29.17) | 17 (70.83) | 24 | |
Moderate | 5 (31.25) | 11 (68.75) | 16 | |
Well | 7 (77.77) | 2 (22.22) | 9 | |
Anatomical Site | p = 0.19 | |||
Palatine tonsils | 7 (26.92) | 19 (73.07) | 26 | |
Tongue base | 7 (50) | 7 (50) | 14 | |
Soft palate | 5 (55.55) | 4 (44.44) | 9 | |
p16 IHC | p = 0.0004 *** | |||
Positive | 5 (17.85) | 23 (82.14) | 28 | |
Negative | 14 (70) | 6 (30) | 20 |
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Oliva, C.; Carrillo-Beltrán, D.; Boettiger, P.; Gallegos, I.; Aguayo, F. Human Papillomavirus Detected in Oropharyngeal Cancers from Chilean Subjects. Viruses 2022, 14, 1212. https://doi.org/10.3390/v14061212
Oliva C, Carrillo-Beltrán D, Boettiger P, Gallegos I, Aguayo F. Human Papillomavirus Detected in Oropharyngeal Cancers from Chilean Subjects. Viruses. 2022; 14(6):1212. https://doi.org/10.3390/v14061212
Chicago/Turabian StyleOliva, Carolina, Diego Carrillo-Beltrán, Paul Boettiger, Iván Gallegos, and Francisco Aguayo. 2022. "Human Papillomavirus Detected in Oropharyngeal Cancers from Chilean Subjects" Viruses 14, no. 6: 1212. https://doi.org/10.3390/v14061212
APA StyleOliva, C., Carrillo-Beltrán, D., Boettiger, P., Gallegos, I., & Aguayo, F. (2022). Human Papillomavirus Detected in Oropharyngeal Cancers from Chilean Subjects. Viruses, 14(6), 1212. https://doi.org/10.3390/v14061212