Galectin-3-ITGB1 Signaling Mediates Interleukin 10 Production of Hepatic Conventional Natural Killer Cells in Hepatitis B Virus Transgenic Mice and Correlates with Hepatocellular Carcinoma Progression in Patients
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals
2.2. Reagents
2.3. Mononuclear Cell (MNC) Preparation
2.4. Purification of cNK Cells
2.5. Flow Cytometry Analysis
2.6. Electron Microscopic Morphology of NK Cells
2.7. Serum Transaminase Activity Assays
2.8. Galectin-3 Binding Analysis
2.9. Galectin-3 Stimulation In Vitro
2.10. Correlation Analysis
2.11. Statistical Analysis
3. Results
3.1. Increased Hepatic cNK Cells Were Skewed to Produce IL-10 and Exhibited Immune Inhibitory Features with Ageing in HBs-Tg Mice
3.2. Hepatic cNK Cells Showed a Distinct Mitochondrial Signature in HBs-Tg Mice
3.3. HBsAg+ Hepatocytes Enhanced Galectin-3 Expression in HBs-Tg Mice
3.4. Galectin-3 Accounted for IL-10 Production in Hepatic cNK Cells and Prevented Liver Injury in HBs-Tg Mice
3.5. Galectin-3 Induced IL-10 Transcription via ITGB1 Signaling in Hepatic cNK Cells
3.6. LGALS3 and ITGB1 Expression Negatively Correlated with the Poor Progression of HCC
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Abbreviations
NK | natural killer cell |
cNK | conventional NK cell |
LrNK | liver-resident NK cell |
NKT | natural killer T |
HBs-Tg | HBV transgenic mice |
HBV | hepatitis B virus |
HBsAg | hepatitis B virus surface antigen |
HCC | hepatocellular carcinoma |
ALT | alanine aminotransferase |
AST | aspartate aminotransferase |
MNC | mononuclear cell |
PBS | phosphate-buffered saline |
mAb | monoclonal antibody |
FITC | fluorescein isothiocyanate |
IFN-γ | interferon-γ |
IL | interleukin |
NKG2D | natural killer cell group 2D |
ITGB1 | integrin β1 |
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Chen, Y.; Zhang, W.; Cheng, M.; Hao, X.; Wei, H.; Sun, R.; Tian, Z. Galectin-3-ITGB1 Signaling Mediates Interleukin 10 Production of Hepatic Conventional Natural Killer Cells in Hepatitis B Virus Transgenic Mice and Correlates with Hepatocellular Carcinoma Progression in Patients. Viruses 2024, 16, 737. https://doi.org/10.3390/v16050737
Chen Y, Zhang W, Cheng M, Hao X, Wei H, Sun R, Tian Z. Galectin-3-ITGB1 Signaling Mediates Interleukin 10 Production of Hepatic Conventional Natural Killer Cells in Hepatitis B Virus Transgenic Mice and Correlates with Hepatocellular Carcinoma Progression in Patients. Viruses. 2024; 16(5):737. https://doi.org/10.3390/v16050737
Chicago/Turabian StyleChen, Yongyan, Wendi Zhang, Min Cheng, Xiaolei Hao, Haiming Wei, Rui Sun, and Zhigang Tian. 2024. "Galectin-3-ITGB1 Signaling Mediates Interleukin 10 Production of Hepatic Conventional Natural Killer Cells in Hepatitis B Virus Transgenic Mice and Correlates with Hepatocellular Carcinoma Progression in Patients" Viruses 16, no. 5: 737. https://doi.org/10.3390/v16050737
APA StyleChen, Y., Zhang, W., Cheng, M., Hao, X., Wei, H., Sun, R., & Tian, Z. (2024). Galectin-3-ITGB1 Signaling Mediates Interleukin 10 Production of Hepatic Conventional Natural Killer Cells in Hepatitis B Virus Transgenic Mice and Correlates with Hepatocellular Carcinoma Progression in Patients. Viruses, 16(5), 737. https://doi.org/10.3390/v16050737