Sylvatic Mosquito Viromes in the Cerrado Biome of Minas Gerais, Brazil: Discovery of New Viruses and Implications for Arbovirus Transmission
Abstract
:1. Introduction
2. Materials and Methods
2.1. Sample Collection
2.2. RNA Extraction and High-Throughput Sequencing
2.3. Bioinformatic Quality Control, Assembly of Contigs, and Viral Detection
2.4. Phylogenetic Analyses
3. Results
3.1. Xinmoviridae
3.2. Phasmaviridae
3.3. Lispiviridae
3.4. Iflaviridae
3.5. Virgaviridae
3.6. Flaviviridae
3.7. Mesoniviridae
3.8. Negevirus
4. Discussion
4.1. Xinmoviridae
4.2. Phasmaviridae
4.3. Lispiviridae
4.4. Iflaviridae
4.5. Virgaviridae
4.6. Flaviviridae
4.7. Mesoniviridae
4.8. Negevirus
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Municipality | Total Mosquitoes per Pool | Species per Pool |
---|---|---|
Brasília de Minas | 21 | Psorophora (Janthinosoma) albipes (Theobald, 1907) |
Icaraí de Minas and Ubaí | 197 | Sabethes (Sabethes) albiprivus (Theobald, 1903) |
Icaraí de Minas, Arinos, Brasília de Minas | 56 | Mixed pool (Sa. albiprivus, Sa. (Sabethoides) chloropterus (von Humboldt, 1819), and Ps. ferox) |
Rio Pardo de Minas | 22 | Psorophora (Janthinosoma) ferox (von Humboldt, 1819) |
Arinos | 92 | Coquillettidia (Rhynchotaenia) venezuelensis (Theobald, 1912) |
Viruses Found in This Study (* New Viruses) | Genome Type | Viral Families | Viral Contigs | Contig Amount | Read Number (Millions) | Insect Pool |
---|---|---|---|---|---|---|
Albipes mosquito Gordis-like virus * | ssRNA− | Xinmoviridae | 1 | 3882 | 20.5 | Ps. albipes |
Sabethes albiprivus phasmavirus * | ssRNA− 1 | Phasmaviridae | 4 | 13,548 | 25.1 | Sa. albiprivus |
Pedras lispivirus variantisolate MG | ssRNA− | Lispividae | ||||
Sabethes albiprivus iflavivirus * | ssRNA+ | Iflaviridae | ||||
Sabethes albiprivus virgavirus 1 * | ssRNA+ | Virgaviridae | ||||
Buriti virga-like virus variantisolate MG | ssRNA+ | Virgaviridae | 1 | 37,238 | 26.2 | Mixed (Sa. albiprivus, Sa. chloropterus, and Ps. ferox) |
Ferox mosquito mononega-like virus * | ssRNA− | Xinmoviridae | 2 | 2859 | 30.4 | Ps. ferox |
Psorophora ferox flavivirus * | ssRNA+ | Flaviviridae | ||||
Alphamesonivirus cavallyense variantisolate MG | ssRNA+ | Mesoniviridae | 3 | 2014 | 25.6 | Cq. venezuelensis |
Biggie virus variantisolate MG | ssRNA+ | Negevirus | ||||
Coquillettidia velezuensis negevirus * |
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Maia, L.J.; Silva, A.B.; Oliveira, C.H.d.; Campos, F.S.; Silva, L.A.d.; Abreu, F.V.S.d.; Ribeiro, B.M. Sylvatic Mosquito Viromes in the Cerrado Biome of Minas Gerais, Brazil: Discovery of New Viruses and Implications for Arbovirus Transmission. Viruses 2024, 16, 1276. https://doi.org/10.3390/v16081276
Maia LJ, Silva AB, Oliveira CHd, Campos FS, Silva LAd, Abreu FVSd, Ribeiro BM. Sylvatic Mosquito Viromes in the Cerrado Biome of Minas Gerais, Brazil: Discovery of New Viruses and Implications for Arbovirus Transmission. Viruses. 2024; 16(8):1276. https://doi.org/10.3390/v16081276
Chicago/Turabian StyleMaia, Luis Janssen, Arthur Batista Silva, Cirilo Henrique de Oliveira, Fabricio Souza Campos, Leonardo Assis da Silva, Filipe Vieira Santos de Abreu, and Bergmann Morais Ribeiro. 2024. "Sylvatic Mosquito Viromes in the Cerrado Biome of Minas Gerais, Brazil: Discovery of New Viruses and Implications for Arbovirus Transmission" Viruses 16, no. 8: 1276. https://doi.org/10.3390/v16081276